Rapid Detection and Quantification of Viable Cells of Using Propidium Monoazide Combined with Real-Time PCR.

() has caused significant economic losses in major vegetable production areas in Northern China by causing bacterial soft rot in cash crops such as potatoes and cucumbers. This study aimed to establish a PMA-qPCR detection method for by screening specific and sensitive primers based on the gene and the conserved region of the 23S rRNA gene. Based on the optimized PMA pretreatment conditions, a standard curve was designed and constructed for PMA-qPCR detection (y = -3.

A mitochondria-targeted fluorescent probe for real-time imaging SO/HO.

Studies have shown that changes in the redox state of cells might be closely related to pathological and physiological processes. Sulfur dioxide and hydrogen peroxide, as a significant redox couple in living cells, are endogenously produced by cells. Here, we report a long-wavelength fluorescent probe to reversibly monitor sulfur dioxide and hydrogen peroxide.

[Effect of dahuang zhechong pill on content of amino acids in hippocampal tissue of ischemic cerebral hemorrhagic rats].

Objective: To observe the effect of Dahuang Zhechong Pill (DZP) on the content of amino acids in hippocampal tissue of model rats of ischemic cerebral hemorrhage (ICH).

Methods: Collagenase and heparin were injected into caudate nucleus to establish ICH rat model. The content of glutamic acid (Glu), aspartic acid (Asp) and gamma-aminobutyric acid (GABA) in hippocampal tissue was detected by anti-phase high-performance liquid chromatographic fluorimetry to observe the effect of DZP on the content of amino acids in brain tissue.

Effects of electroacupuncture on the contents of NO, ET and T-AOC in the brain tissues of the cerebral hemorrhage model rats.

In the cerebral hemorrhage model rats established by injection of collagenase and heparin into caudate nucleus, the effects of electroacupuncture (EA) on the contents of nitric oxide (NO) and endothelin (ET), and total anti-oxidation capability (T-AOC) in the brain tissues were investigated. It is found that the content of NO in the Shuigou EA group lowered, ET decreased and the T-AOC raised significantly in both the Fengfu EA group and the Shuigou EA group (all P<0.05) as compared with the model group, indicating that acupuncture can reduce the contents of ET and NO, and increase the T-AOC in the brain tissues of the rats with cerebral hemorrhage.

[Effect of Chinese and western medicine integration on spinning behavior of rats with Parkinson disease].

Objective: To observe the effect of Chinese and western medicine integration on the spinning behavior of rats with Parkinson disease.

Methods: Model of the lateral Parkinson disease was made with injection of 6-hydroxydopamine (6-OHDA) into the black substance of the right side of the brain, and the model rats were treated with madopar and Chinese herbs for nourishing the liver and kidney, clearing collaterals and detoxification. The rat's spinning behavior was observed, and was compared with the normal control group, madopar group and sham-operation group at the same time.

[Clinical randomized double-blinded study on treatment of vascular dementia by jiannao yizhi granule].

Objective: To observe the effect of Chinese herbal medicine in treating vascular dementia (VD) and to explore the method for therapeutic effect evaluation.

Methods: Multi-center, double-blinded randomize-controlled method was adopted to observe the effect of treatment of 242 patients with VD of mild or middle degree, they were 89 cases in the TCM group, 106 cases in the western medicine group and 47 in the placebo group. Minimental state examination (MMSE), Blessed dementia scoring on TCM symptom classified evaluation were used to evaluate the therapeutic effect after 60 days treatment.