The Deciphering of Growth-Dependent Strategies for Quorum-Sensing Networks in .

is recognized as a significant cause of morbidity and mortality among nosocomial pathogens. In respiratory infections, acts not only as a single player but also collaborates with the opportunistic fungal pathogen . This study introduced a QS molecule portfolio as a potential new biomarker that affects the secretion of virulence factors and biofilm formation.

Siderophore-Based Noninvasive Differentiation of Aspergillus fumigatus Colonization and Invasion in Pulmonary Aspergillosis.

Germination from conidia to hyphae and hyphal propagation of Aspergillus fumigatus are the key pathogenic steps in the development of invasive pulmonary aspergillosis (IPA). By applying observations in a clinical study of 13 patients diagnosed with probable IPA, here, we show that the transition from colonization to the A. fumigatus invasive stage is accompanied by the secretion of triacetylfusarinine C (TafC), triacetylfusarinine B (TafB), and ferricrocin (Fc) siderophores into urine, with strikingly better sensitivity performance than serum sampling.

Correction: Patil et al. Freeing of Polymycovirus Infection Renders It More Resistant to Competition with Due to Altered Iron-Acquiring Tactics. 2021, , 497.

In the original publication [...

Infection metallomics for critical care in the post-COVID era.

Infection metallomics is a mass spectrometry (MS) platform we established based on the central concept that microbial metallophores are specific, sensitive, noninvasive, and promising biomarkers of invasive infectious diseases. Here we review the in vitro, in vivo, and clinical applications of metallophores from historical and functional perspectives, and identify under-studied and emerging application areas with high diagnostic potential for the post-COVID era. MS with isotope data filtering is fundamental to infection metallomics; it has been used to study the interplay between "frenemies" in hosts and to monitor the dynamic response of the microbiome to antibiotic and antimycotic therapies.

Freeing of Polymycovirus Infection Renders It More Resistant to Competition with Due to Altered Iron-Acquiring Tactics.

A virus-free (VF) isolate has been shown to be resistant in competition with as compared to the isogenic line infected with polymycovirus 1 (AfuPmV-1), and this phenotype was apparently related to alterations in iron metabolism. Here we investigated further the mechanisms underpinning this phenotype. The extracellular siderophore profiles of five isogenic VF and virus-infected (VI) strains were sampled at 24, 31, 48, 54, and 72 h in submerged cultures and quantitatively examined by liquid chromatography and mass spectrometry.

Rhizoferrin Glycosylation in .

spp. are the most common etiological agents of mucormycosis, causing over 90% mortality in disseminated infections. The diagnosis relies on histopathology, culture, and/or polymerase chain reaction.