Improving Care Delivery: Location Timestamps to Enhance Process Measurement of a Clinical Workflow.

Unlabelled: Traditional quality improvement (QI) strategies to describe workflow processes rely primarily upon qualitative methods or human-driven observations. These methods may be limited in scope and accuracy when applied to time-based workflow processes. This study sought to evaluate the utility of integrating objective time measurements to augment traditional QI strategies using procedural sedation workflow in a pediatric emergency department as an archetype.

Functional Coupling between the Unfolded Protein Response and Endoplasmic Reticulum/Golgi Ca-ATPases Promotes Stress Tolerance, Cell Wall Biosynthesis, and Virulence of Aspergillus fumigatus.

Many species of pathogenic fungi deploy the unfolded protein response (UPR) to expand the folding capacity of the endoplasmic reticulum (ER) in proportion to the demand for virulence-related proteins that traffic through the secretory pathway. Although Ca plays a pivotal role in ER function, the mechanism by which transcriptional upregulation of the protein folding machinery is coordinated with Ca homeostasis is incompletely understood. In this study, we investigated the link between the UPR and genes encoding P-type Ca-ATPases in the human-pathogenic mold We demonstrate that acute ER stress increases transcription of the gene, encoding a member of the sarco/endoplasmic reticulum Ca-ATPase (SERCA) family, as well as that of , encoding a secretory pathway Ca-ATPase (SPCA) in the Golgi membrane.