A tomato alternative oxidase protein with altered regulatory properties.

We have investigated the expression and regulatory properties of the two alternative oxidase (Aox) proteins that are expressed in tomato (Lycopersicon esculentum L. Mill cv. Sweetie) after storage of green fruit at 4 degrees C.

Mitochondrial protein expression in tomato fruit during on-vine ripening and cold storage.

We have investigated the activity and abundance of a number of respiratory chain components in ripening and cold-treated tomato fruits (Lycopersicon esculentum L. Mill cvv. Moneymaker and Sweetie).

Supply of O2 regulates demand for O2 and uptake of malate by N2-fixing bacteroids from soybean nodules.

Bacteroids, prepared anaerobically from soybean root nodules by fractional centrifugation or by sucrose or Percoll density-gradient methods, were retained within a stirred, flow-through reaction chamber and used to determine rates of respiration and N2 fixation at various rates of O2 supply. Liquid reaction solutions containing malate, oxyleghaemoglobin, dissolved N2 and various levels of dissolved O2 were passed through the reaction chamber at measured rates of flow. The relative oxygenation of leghaemoglobin in the chamber was determined automatically by spectrophotometry of the effluent solution, and the concentrations of free, dissolved O2 ([O2(free)]) and the rates of O2 consumption were calculated.