Development and validation of the stability indicating RP-UHPLC method for the determination of the chemical purity and assay of bimatoprost.

A simple, rapid and accurate ultra-high performance liquid chromatographic (UHPLC) method with a UV detection for the determination of the chemical purity and assay of bimatoprost (BT-1) was developed. The chromatographic separation was achieved with the use of an Acquity BEH C8, 150 × 2.1 mm, 1.

Evidence of a weak dipole transition moment between the X(1)0g+ and (3)1u(5(3)P1) electronic energy states in Cd2.

Theoretical and experimental evidence of a weak M(z)(R) dipole transition moment between the X(1)0g+ ground and (3)1u(5(3)P1) excited states in Cd2 is presented. Two independent attempts at recording an excitation spectrum of the (3)1u <-- X(1)0g+ transition using a laser beam crossed with a supersonic free-jet expansion beam are reported. The measurements were performed in a spectral range predicted as a result of both ab initio calculations of the electronic energy-state potentials involved in the transition and a simulation of the excitation spectrum.