Possible detection of physiological state disorders with the help of medicinal leeches Hirudo verbana Carena, 1820.

In the experiment, 160 medicinal leeches of the species Hirudo verbana Carena, 1820 were studied. Medicinal leeches were fed on the blood of animals and people (conditionally healthy and diseased). Four leeches were taken from each animal/person.

The effect of blood human, domestic and small laboratory animals on the viability and behavior of the medicinal leech.

Medicinal leeches are used for therapeutic purposes in the prevention and treatment of many diseases. Because they have a large amount of biologically active substances in their body. Each of these substances has many therapeutic effects.

The duration of rest and feeding greatly affects the re-breeding of ectoparasites: Hirudo verbana, Hirudo medicinalis and Hirudo orientalis.

Ectoparasitic leeches have many biologically active substances in their body, which are able to show various therapeutic effects, which makes them very relevant in the study. Among them, the most common are medicinal leeches: Hirudo verbana, Hirudo medicinalis and Hirudo orientalis. They are listed in the Red Book as a vulnerable species, so their population is mostly supported in biolabs.

The influence of the planting time and conditions on the reproductive properties of Hirudo verbana and Hirudo medicinalis.

Factors that affect on the sexual behavior of leeches not only in nature, but also in artificial conditions remain largely unexplored. We did not find data on the behavior of medicinal leeches, which are not placed in time from the aquatic environment in the peat-soil conditions after the appearance of clitella on their bodies. Therefore, the study of this problem has become relevant.

Morphological parameters of spleen and thymus of the male rats on the basis of the hirudological influence of Hirudo verbana.

The aim of this work is to study the morphological parameters of the spleen and thymus of rats along with hirudological influence from Hirudo verbana. The work was carried out on 40 male outbred laboratory rats weighing 280-300 grams, age 6.5-7 months.

Indirect effect of substances of the hemophagous parasite Hirudo verbana on the immune system of the host rats.

A positive relationship between the hematophagous parasite-medicinal leech and the host (animals and humans) has been partially shown by many authors. But only direct influence was shown, and how they can influence indirectly has not been studied by anyone. Therefore, it became relevant to analyse the immunological parameters of blood and histological analysis of the central lymphoid organ of female rats on the 30th day after the birth of their offspring, as well as the same indicators in their offspring on the 30th and 60th days of ontogenesis under the indirect influence of saliva substances of the hematophagous parasite Hirudo verbana.

The impact of fetal load of Hirudo verbana saline extract antigens on morphometrical, hematological and immunological parameters of rats in the early stages of post-embryonic development.

The aim of the study was to determine the immunostimulatory effect of antigens from saline extract of the medicinal leech on the morphometric parameters of the body and major immunogenic organs of rats. Wild-type female rats were administered medicinal leech saline extract antigens intraperitoneally two weeks before and two weeks after mating (in total four injections). The offspring were examined, as were the mothers after feeding the offspring, at 1, 15, 30, 60 days after birth.