HIV Replication Under High-Level Cabotegravir Is Associated with the Appearance of 3'-PPT Mutations, Circular DNA Transcription and Recombination.

The HIV integrase inhibitor, dolutegravir (DTG), in the absence of eliciting integrase (int) resistance, has been reported to select mutations in the virus 3'-polypurine tract (3'-PPT) adjacent to the 3'-LTR U3. An analog of DTG, cabotegravir (CAB), has a high genetic barrier to drug resistance and is used in formulations for treatment and long-acting pre-exposure prophylaxis. We examined whether mutations observed for DTG would emerge in vitro with CAB.

The Impact of Genetic Variation on Duck Hepatitis A Virus (DHAV) Vaccine Efficacy: A Comparative Study of DHAV-1 and DHAV-3 Against Emerging Variant Strains.

Duck virus hepatitis (DVH), caused by duck hepatitis A virus (DHAV), poses significant challenges to duck farming due to high mortality rates in young ducklings. Despite the widespread use of live attenuated vaccines, the genetic diversity within DHAV strains has diminished their cross-protection efficacy. This study aimed to evaluate the cross-protective efficacy of current DHAV-1 and DHAV-3 vaccines against genetically divergent wild strains.

Oral Yeast-Cell Microcapsule-Mediated DNA Vaccines Against Induce Effective Intestinal Immunity and Modulate Gut Microbiota.

is a common opportunistic pathogen that causes gastrointestinal diseases in livestock and poultry. Our preliminary research has demonstrated that administering oral yeast-cell microcapsule (YCM)-mediated DNA vaccines can effectively stimulate mucosal immunity, thereby preventing the occurrence of gastrointestinal diseases. In this study, the α-toxin gene was first cloned and the H126G and C-terminal (C247-370) mutations were created.

A User Association and Resource Allocation Algorithm for UAV-Assisted Smart Grid.

Recently, massive intelligent applications have emerged for the smart grid (SG), such as inspection and sensing. To support these applications, there have been high requirements on wireless communication for the SG, especially in remote areas. To tackle these challenges, a UAV-assisted heterogeneous wireless network is proposed in this paper for the SG, where multiple UAVs and a macro base station collaboratively provide a wide range of communication services.

Review on Portable-Powered Lower Limb Exoskeletons.

Advancements in science and technology have driven the growing use of robots in daily life, with Portable-Powered Lower Limb Exoskeletons (PPLLEs) emerging as a key innovation. The selection of mechanisms, control strategies, and sensors directly influences the overall performance of the exoskeletons, making it a crucial consideration for research and development. This review examines the current state of PPLLE research, focusing on the aspects of mechanisms, control strategies, and sensors.

In-Motion Forward-Forward Backtracking Fine Alignment Based on Displacement Observation for SINS/GNSS.

To solve the problem of slow convergence seen in the traditional fine alignment algorithm based on linear Kalman filtering, a forward-forward backtracking fine alignment algorithm for SINS is proposed after reanalyzing the fine alignment model in this paper. First, the forward-forward backtracking fine alignment model in initial navigation frame was derived. The displacement vector of the carrier in the initial navigation frame solved by GNSS positioning was utilized as the observation of the fine alignment model.

A Multifunctional Nanozyme Hydrogel with Antibacterial, Antioxidative, and Photo-Induced Nitric Oxide-Supplying Properties for Promoting Infected Wound Healing.

To design a multifunctional nanozyme hydrogel with antibacterial, photo-responsive nitric oxide-releasing, and antioxidative properties for promoting the healing of infected wounds. We first developed ultra-small silver nanoparticles (NPs)-decorated sodium nitroprusside-doped Prussian blue (SNPB) NPs, referred to as SNPB@Ag NPs, which served as a multifunctional nanozyme. Subsequently, this nanozyme, together with geniposide (GE), was incorporated into a thermo-sensitive hydrogel, formulated from Poloxamer 407 and carboxymethyl chitosan, creating a novel antibacterial wound dressing designated as GE/SNPB@Ag hydrogel.

C118P Suppresses Gastric Cancer Growth via Promoting Autophagy-Lysosomal Degradation of RAB1A.

: Gastric cancer (GC) is the leading cause of cancer-related deaths worldwide. C118P, a microtubule inhibitor with anti-angiogenic and vascular-disrupting activities, was proven to be cytotoxic to various cancer cell lines. This study aimed to explore the anti-tumor effect of C118P against gastric cancer and identify its potential target.

Synthesis of a Bimetallic-Doped Phytate-Melamine Composite as an Efficient Additive for Epoxy Resins with High Fire Safety.

The issue of hazardous smoke and toxic gases released from epoxy resins (EP), which often causes casualties in real fires, has limited its application. Therefore, we have developed a novel flame retardant based on a bimetallic-doped phytate-melamine (BPM) structure with Zn and Fe ions incorporated into the polymer matrix using a straightforward solution-based synthetic method. The combustion performance of the composite was evaluated using a cone calorimeter test, which showed that the peak heat release, total heat release, and total smoke production were reduced by 50%, 31.

Influence of Operating Temperature on Crack Growth Characteristics and Fatigue Life Prediction of Carbon Black-Filled Hydrogenated Nitrile Butadiene Rubber.

Rubber is widely used in situations involving cyclic loads, and the influence of temperature on rubber properties is particularly pronounced under cyclic loading. In this study, mechanical property tests and crack propagation tests of carbon black-filled hydrogenated nitrile butadiene rubber were conducted at four different operating temperatures. Based on the results of the crack propagation tests, the temperature-dependent characteristics of the Paris-Erdogan parameters and strain energy density were clarified.

A Photothermal-Responsive Soft Actuator Based on Biomass Carbon Nanosheets of Synergistic Bilateral Polymers.

Currently, polymer actuators capable of photothermal response are being developed to be more sensitive and repeatable. In this work, a three-layered structured soft film actuator (NA/PET/NI-3) was designed by combining poly(N-isopropylacrylamide) (PNIPAM), poly(N-(2-aminoethyl)-acrylamide) (PANGA) and poly(ethylene glycol-co-terephthalate) (PET) film. Coconut water and PEI were used to synthesize a new kind of carbon nanosheet (PEI-CCS), which, when triggered by near-infrared light, will enable photothermal bending behavior in the micrometer-scale NA/PET/NI-n film, while PET served as the supporting and heat conducting layer.

Influence of Wild and Cultivated Environments on the Antioxidant and Medicinal Components of A. Boriss.

A. Boriss., recognized for its significant medicinal potential, is increasingly threatened by overharvesting in wild habitats.

Superellipse Equation Describing the Geometries of Tree Rings.

Our previous study using 41 tree rings of one Mill. disc indicated that the superellipse equation can accurately fit its tree-ring shape. This study further used the superellipse equation (xan+yβn=1 ) to model the geometries of 1090 tree rings of discs collected from five sites in Denmark.

Development and Validation of Kompetitive Allele-Specific Polymerase Chain Reaction Markers for Seed Protein Content in Soybean.

Seed protein content is a critical trait in soybean breeding, as it provides a primary source of high-quality protein for both human consumption and animal feed. This study aimed to enhance molecular marker-assisted selection for high-protein soybean varieties by developing Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) markers targeted at loci associated with seed protein content. Nineteen markers with high genotyping efficacy were identified through screening.

Subclinical Mastitis in Small-Holder Dairy Herds of Gansu Province, Northwest China: Prevalence, Bacterial Pathogens, Antimicrobial Susceptibility, and Risk Factor Analysis.

This cross-sectional study assessed the prevalence, bacterial distribution, antimicrobial susceptibility, and potential risk factors associated with subclinical mastitis (SCM) in small-holder dairy herds in Gansu Province, Northwest China. Forty small-holder cow farms were randomly selected from eight cities/counties in six districts of Gansu Province, and a total of = 530 lactating cows were included in this study. SCM prevalence was noted at 38.

Nitrogen-Fixing and : Two Novel Species from Plant Rhizospheres.

Two strains, M1 and H32 with nitrogen-fixing ability, were isolated from the rhizospheres of different plants. Genome sequence analysis showed that a (trogen ixation) gene cluster composed of nine genes () was conserved in the two strains. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strains M1 and H32 are members of the genus .

One-Step Multiplex Real-Time Fluorescent Quantitative Reverse Transcription PCR for Simultaneous Detection of Four Waterfowl Viruses.

Duck Tembusu virus (DTMUV), duck hepatitis virus (DHV), Muscovy duck reovirus (MDRV), and Muscovy duck parvovirus (MDPV) represent four emergent infectious diseases impacting waterfowl, which can be challenging to differentiate due to overlapping clinical signs. In response to this, we have developed a one-step multiplex real-time fluorescence quantitative reverse transcription PCR (qRT-PCR) assay, capable of simultaneously detecting DTMUV, DHV, MDRV, and MDPV. This method exhibits high specificity, avoiding cross-reactivity with other viruses such as Fowl adenoviruses (FADV), infectious bursal disease virus (IBDV), infectious bronchitis virus (IBV), infectious laryngotracheitis virus (ILTV), Haemophilus paragallinarum (Hpg), duck circovirus (DUCV), goose astrovirus (GoAstV), and mycoplasma gallisepticum (MG).

Metagenome-Assembled Genomes of Pig Fecal Samples in Nine European Countries: Insights into Antibiotic Resistance Genes and Viruses.

Gut microbiota plays a crucial role in the health and productivity of pigs. However, the spread of antibiotic resistance genes (ARGs) and viruses within the pig intestinal microbiota poses significant threats to animal and public health. This study utilized 181 pig samples from nine European countries and employed metagenomic assembly methods to investigate the dynamics and distribution of ARGs and viruses within the pig intestinal microbiota, aiming to observing their associations with potential bacterial hosts.

Design and Implementation of a CMOS-MEMS Out-of-Plane Detection Gyroscope.

A MEMS gyroscope is a critical sensor in attitude control platforms and inertial navigation systems, which has the advantages of small size, light weight, low energy consumption, high reliability and strong anti-interference capability. This paper presents the design, simulation and fabrication of a Y-axis gyroscope with out-of-plane detection developed using CMOS-MEMS technology. The structural dimensions of the gyroscope were optimized through a multi-objective genetic algorithm, and modal, harmonic response and range simulation analyses were carried out to verify the reasonableness of the design.

Application of Solution-Processed High-Entropy Metal Oxide Dielectric Layers with High Dielectric Constant and Wide Bandgap in Thin-Film Transistors.

High-k metal oxides are gradually replacing the traditional SiO dielectric layer in the new generation of electronic devices. In this paper, we report the production of five-element high entropy metal oxides (HEMOs) dielectric films by solution method and analyzed the role of each metal oxide in the system by characterizing the film properties. On this basis, we found optimal combination of (AlGaTiYZr)O with the best dielectric properties, exhibiting a low leakage current of 1.

Silicon Extraction from a Diamond Wire Saw Silicon Slurry with Flotation and the Flotation Interface Behavior.

Diamond wire saw silicon slurry (DWSSS) is a waste resource produced during the process of solar-grade silicon wafer preparation with diamond wire sawing. The DWSSS contains 6N grade high-purity silicon and offers a promising resource for high-purity silicon recycling. The current process for silicon extraction recovery from DWSSS presents the disadvantages of lower recovery and secondary pollution.

Investigation on the Coordination Bonding Nature of Actinide-Doped Endohedral Borospherenes An@B (An = U, Np, Pu, Am, Cm).

Endohedral metallo-borospherenes M@B have received considerable attention since the discovery of B in 2014. However, the coordination bonding nature of most of actinide-doped endohedral An@B still remains in dispute or unexplored. Extensive and systematic first-principles theory calculations performed herein unveil the ground states of triplet U@B (, , A), quartet U@B (, , B), quintet Np@B (, , A), sextet Np@B (, , A), septet Pu@B (, , A), octet Am@B (, , A), and octet Cm@B (, , A) at the coupled-cluster with triple excitations CCSD(T) level.

Research on the Adsorption Mechanism and Performance of Cotton Stalk-Based Biochar.

In this research, we produced two types of biochar (BC) using cotton stalks as raw material and KOH as an activator, and compared their performance and adsorption mechanisms in the removal of tetracycline (TC) and methylene blue (MB) from wastewater. The results showed that the biochar generated using both procedures formed pores that connected to the interior of the biochar and had extensive microporous and mesoporous structures. The molten salt approach produces biochar with a higher specific surface area, larger pore size, and higher pore volume than the impregnation method, with a maximum specific surface area of 3095 m/g.

Annealing Effect on Linear and Ultrafast Nonlinear Optical Properties of BiTe Thin Films.

In recent years, the fabrication of materials with large nonlinear optical coefficients and the investigation of methods to enhance nonlinear optical performance have been in the spotlight. Herein, the bismuth telluride (BiTe) thin films were prepared by radio-frequency magnetron sputtering and annealed in vacuum at various temperatures. The structural and optical properties were characterized and analyzed using X-ray diffraction, scanning electron microscopy, X-ray photoelectron spectroscopy, spectroscopic ellipsometry, and UV/VIS/NIR spectrophotometry.

Effects of Temperature and Water Vapor Content on Microstructure, Mechanical Properties and Corrosion Behavior of C/C-SiC Composites.

Carbon-fiber-reinforced carbon and silicon carbide (C/C-SiC) composites were prepared using chemical vapor infiltration (CVI) combined with reactive melt infiltration (RMI). The microstructure and flexural properties of C/C-SiC composites after oxidation in different temperature water vapor environments were studied. The results indicate that the difficulty of oxidation in water vapor can be ranked from easy to difficult in the following order: carbon fiber (CF), pyrolytic carbon (PyC), and ceramic phase.