Cell wall canals formed upon growth of Candida maltosa in the presence of hexadecane are associated with polyphosphates.

Canals are supramolecular complexes observed in the cell wall of Candida maltosa grown in the presence of hexadecane as a sole carbon source. Such structures were not observed in glucose-grown cells. Microscopic observations of cells stained with diaminobenzidine revealed the presence of oxidative enzymes in the canals.

Modifications of the cell wall of yeasts grown on hexadecane and under starvation conditions.

Electron-microscopic examinations have demonstrated local modifications in the cell wall of the yeast Candida maltosa grown on hexadecane. In our earlier studies, these modified sites, observed in other yeasts grown on oil hydrocarbons, were conventionally called 'canals'. The biochemical and cytochemical studies of C.

Manganese tolerance in yeasts involves polyphosphate, magnesium, and vacuolar alterations.

Basidiomycetous and ascomycetous yeast species were tested for manganese tolerance. Basidiomycetous Cryptococcus humicola, Cryptococcus terricola, Cryptococcus curvatus and ascomycetous Candida maltosa, Kluyveromyces marxianus, Kuraishia capsulata, Lindnera fabianii and Sacharomyces cerevisiae were able to grow at manganese excess (2.5 mmol/L), while the growth of basidiomycetous Rhodotorula bogoriensis was completely suppressed.

[Clinical efficacy of Longidaze for the prevention of cicatricial and sclerotic complications after surgery on the upper urinary tract].

Results of surgical treatment of patients with ureteral obstruction are not always successful. Prevention of cicatricial and sclerotic complications in the postoperative period plays an important role. In this connection, search for pathogenetic methods that prevent the recurrence of strictures is one of the urgent problems of modern urology.

Microorganisms form exocellular structures, trophosomes, to facilitate biodegradation of oil in aqueous media.

Cytochemical staining and microscopy were used to study the trophic structures and cellular morphotypes that are produced during the colonization of oil-water interfaces by oil-degrading yeasts and bacteria. Among the microorganisms studied here, the yeasts (Schwanniomyces occidentalis, Torulopsis candida, Candida tropicalis, Candida lipolytica, Candida maltosa, Candida paralipolytica) and two representative bacteria (Rhodococcus sp. and Pseudomonas putida) produced exocellular structures composed of biopolymers during growth on petroleum hydrocarbons.

[Electron microscopic detection and in situ characterization of bacterial nanoforms in extreme biotopes].

The morphology, ultrastructure, and quantity of bacterial nanoforms were studied in extreme biotopes: East Siberia permafrost soil (1-3 Ma old), petroleum-containing slimes (35 years old), and biofilms from subsurface oil pipelines. The morphology and ultrastructure of microbial cells in natural biotopes in situ were investigated by high-resolution transmission electron microscopy and various methods of sample preparation: ultrathin sectioning, cell replicas, and cryofractography. It was shown that the biotopes under study contained high numbers of bacterial nanoforms (29-43% of the total number of microorganisms) that could be assigned to ultramicrobacteria due to their size (diameter of < or =0.

[New criteria of inflammatory-reparative processes in the region of splenic injury caused by blunt objects].

We propose new criteria of healing of splenic injuries. Such criteria allow a detailed follow-up of the inflammatory-reparative process. We have developed an evaluation table.

[The role of histological examination in timing of the splenic injury].

Basing on her findings at forensic-histological investigations of splenic injuries, the author describes histological inflammatory-reparative alterations in splenic tissue in the rupture regions regarding time parameters. The investigations allow prescription of splenic damage.

[Blood reinfusion as an indicator of time-limit in the development of splenic injuries].

Literature data are analysed on the causes of differencies often encountered in practice between the time of injury by histological examination and time established in the course of legal investigation. An indirect marker--blood reinfusion--can limit the time of splenic damage creation.

[Characterization of Vibrio cholerae eltor in the city of Kazan in 2001].

Information on V. cholerae eltor isolated in the focus of cholera in Kazan in 2001 at different periods of the outbreak is presented. The identity of strains isolated from patients, vibriocarriers and environmental objects, including their antibioticograms (sensitivity to cyprofloxacin and resistance to trimethoprim--sulfamethoxazole, streptomycin, furazolidone and nalidixic acid, which may be regarded as markers), is shown.

[Methanotrophic communities in the soils of Russian northern taiga and subarctic tundra].

The PCR analysis of DNA extracted from soil samples taken in Russian northern taiga and subarctic tundra showed that the DNA extracts contain genes specific to methanotrophic bacteria, i.e., the mmoX gene encoding the conserved alpha-subunit of the hydroxylase component of soluble methane monooxygenase, the pmoA gene encoding the alpha-subunit of particulate methane monooxygenase, and the mxaF gene encoding the alpha-subunit of methanol dehydrogenase.