Exploring the gut microbiota and metabolome of Lateolabrax japonicus: A multi-omics approach.

The intestinal microbiota plays a crucial role in the health and development of fish, engaging in intricate interactions with the host organism. As a significant species in aquaculture, Lateolabrax japonicus serves as an exemplary model for investigating these interactions and their subsequent effects on growth and health. This study utilized a multi-omics approach, incorporating metagenomic sequencing and non-targeted metabolomics, to delineate the gut microbiota and metabolome of L.

Rapid detection of in based on CRISPR-Cas13a coupled with recombinase polymerase amplification.

The principal pathogen responsible for chronic urinary tract infections, immunocompromised hosts, and cystic fibrosis patients is , which is difficult to eradicate. Due to the extensive use of antibiotics, multidrug-resistant has evolved, complicating clinical therapy. Therefore, a rapid and efficient approach for detecting strains and their resistance genes is necessary for early clinical diagnosis and appropriate treatment.

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria.

Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), which is home to several different outer membrane proteins (Omps). One class of important Omps is porins, which mediate the inflow of nutrients and several antimicrobial drugs.

N,N-dimethyl chitosan oligosaccharide (DMCOS) promotes antifungal activity by causing mitochondrial damage.

By modifying chitosan oligosaccharide (COS) with the Eschweiler-Clarke reaction, the chitosan oligosaccharide derivative DMCOS was synthesized. FT-IR, 1D and 2D NMR spectra, MALDI-ToF MS, and elemental analysis were applied to analyze the structure of DMCOS, which revealed that the primary amines were converted into tertiary amines after methylation. DMCOS displayed less thermal stability than COS.

Microbiologically Influenced Corrosion of Q235 Carbon Steel by sp.

The biological sulfur cycle is closely related to iron corrosion in the natural environment. The effect of the sulfur-oxidising bacterium sp., named PHS-Q, on the metal corrosion behaviour rarely has been investigated.

The role of structural evolution in the complexation and flocculation of heavy metals by the microbial product poly-γ-glutamic acid.

The process and mechanism of heavy metal flocculation with extracellular polymeric substances (EPS) secreted by microorganisms, are crucial to their fate in natural environment, wastewater treatment and soil bioremediation applications. However, the structural features of EPS and the relationship between these features and the flocculation process and mechanism remain unclear. In the present study, structural features of the microbial product poly-γ-glutamic acid (γ-PGA) complexed with the heavy metal ions Pb and Cu were characterized and the evolution of these features was identified as having a key role in the flocculation process and mechanism.

Macroporous zwitterionic composite cryogel based on chitosan oligosaccharide for antifungal application.

Chitosan oligosaccharide (COS), a time-dependent antimicrobial carbohydrate, is found antifungal active with a short duration of action due to excessive solubility. We attempted to address this issue by employing a hydrogel as a COS carrier. In this research, macroporous zwitterionic composite cryogels composed of COS and poly(N-methacryl arginine) (PMarg) were fabricated, serving as long-term antifungal dressings.