WEBA dataset as the Reflection of Work content effect on Workload perception in Real life Working conditions.

The effect of work content on workload, stress, and performance was not well addressed in the literature, due to the lack of comprehensive conceptualization, problem definition, and relevant dataset. The gap between laboratory-simulated studies and real-life working conditions delays the generalization, hindering the development of performance management and monitoring tools. Contributing to this topic, a data collection effort is organized, which considers unique work conditions and work content factors of a coffee shop, to conceptualize scenarios that better highlight their effect on human performance, thus creating the Work content Effect on BAristas (WEBA) dataset.

Evaluating Patient Participation in Clinical Trials for CLL and SLE in Germany-A Mixed-Methods Study on Enrollment Potential Based on Claims Data.

In recent years, the pharmaceutical industry in Germany has faced a significant decline in the number of clinical trials conducted. This study evaluates patient participation in clinical trials for oncology and chronic diseases in Germany, integrating quantitative and qualitative research. Data from the Institute for Applied Health Research Berlin (InGef (Institut für angewandte Gesundheitsforschung, Berlin, Germany)), covering about 88% of the German population, and expert interviews were used.

Dinuclear Dysprosium Compounds: The Importance of Rigid Bridges.

We report the synthesis, structures and magnetic behaviour of two isostructural dinuclear Dy complexes where the metal ions of a previously reported monomeric building block are connected by a peroxide (O ) or a pair of fluoride (2×F) bridges. The nature of the bridge determines the distance between the metal ion dipoles leading to a dipolar coupling in the peroxido bridged compound of only ca. 70 % of that in the bis-fluorido bridged dimer.

Infection length and host environment influence on Plasmodium falciparum dry season reservoir.

Persistence of malaria parasites in asymptomatic hosts is crucial in areas of seasonally-interrupted transmission, where P. falciparum bridges wet seasons months apart. During the dry season, infected erythrocytes exhibit extended circulation with reduced cytoadherence, increasing the risk of splenic clearance of infected cells and hindering parasitaemia increase.

CRKL Enhances YAP Signaling through Binding and JNK/JUN Pathway Activation in Liver Cancer.

The Hippo pathway transducers yes-associated protein (YAP) and WW-domain containing transcription regulator 1 (WWTR1/TAZ) are key regulators of liver tumorigenesis, promoting tumor formation and progression. Although the first inhibitors are in clinical trials, targeting the relevant upstream regulators of YAP/TAZ activity could prove equally beneficial. To identify regulators of YAP/TAZ activity in hepatocarcinoma (HCC) cells, we carried out a proximity labelling approach (BioID) coupled with mass spectrometry.

Data reconciliation of indoor positioning data: Improve position data accuracy in warehouse environment.

This article focuses on improving indoor positioning data through data reconciliation. Indoor positioning systems are increasingly used for resource tracking to monitor manufacturing and warehouse processes. However, measurement errors due to noise can negatively impact system performance.

Particle filtering supported probability density estimation of mobility patterns.

This paper presents a methodology that aims to enhance the accuracy of probability density estimation in mobility pattern analysis by integrating prior knowledge of system dynamics and contextual information into the particle filter algorithm. The quality of the data used for density estimation is often inadequate due to measurement noise, which significantly influences the distribution of the measurement data. Thus, it is crucial to augment the information content of the input data by incorporating additional sources of information beyond the measured position data.

Nα-acetyltransferase NAA50 mediates plant immunity independent of the Nα-acetyltransferase A complex.

In humans and plants, 40% of the proteome is cotranslationally acetylated at the N-terminus by a single Nα-acetyltransferase (Nat) termed NatA. The core NatA complex is comprised of the catalytic subunit Nα-acetyltransferase 10 (NAA10) and the ribosome-anchoring subunit NAA15. The regulatory subunit Huntingtin Yeast Partner K (HYPK) and the acetyltransferase NAA50 join this complex in humans.

Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation.

Protein glycosylation is an essential post-translational modification in all domains of life. Its impairment in humans can result in severe diseases named congenital disorders of glycosylation (CDGs). Most of the glycosyltransferases (GTs) responsible for proper glycosylation are polytopic membrane proteins that represent challenging targets in proteomics.

Cell-free DNA extraction from urine of lung cancer patients and healthy individuals: Evaluation of a simple method using sample volume up-scaling.

Background: Urine holds promise as a source for cell-free DNA (cfDNA) analysis of cancer genetics due to its nature as a self-collectable biospecimen available in large quantities. However, pre-analytical variables such as preservation of cfDNA or efficiency of up-scaling specimen volume need to be better explored to increase analysis sensitivity.

Patients And Methods: Initially effects of pH levels on cfDNA stability of urine preserved with EDTA were investigated in three healthy probands.

The human-centric Industry 5.0 collaboration architecture.

While the primary focus of Industry 4.0 revolves around extensive digitalization, Industry 5.0, on the other hand, seeks to integrate innovative technologies with human actors, signifying an approach that is more value-driven than technology-centric.

α-catenin interaction with YAP/FoxM1/TEAD-induced CEP55 supports liver cancer cell migration.

Background: Adherens junctions (AJs) facilitate cell-cell contact and contribute to cellular communication as well as signaling under physiological and pathological conditions. Aberrant expression of AJ proteins is frequently observed in human cancers; however, how these factors contribute to tumorigenesis is poorly understood. In addition, for some factors such as α-catenin contradicting data has been described.

Immunodominant surface epitopes power immune evasion in the African trypanosome.

The African trypanosome survives the immune response of its mammalian host by antigenic variation of its major surface antigen (the variant surface glycoprotein or VSG). Here we describe the antibody repertoires elicited by different VSGs. We show that the repertoires are highly restricted and are directed predominantly to distinct epitopes on the surface of the VSGs.

3D Scanner-Based Identification of Welding Defects-Clustering the Results of Point Cloud Alignment.

This paper describes a framework for detecting welding errors using 3D scanner data. The proposed approach employs density-based clustering to compare point clouds and identify deviations. The discovered clusters are then classified according to standard welding fault classes.

Analysis of the phosphoproteome of CK2/Δ C2C12 myoblasts compared to the wild-type cells.

CK2 is a Ser/Thr protein kinase composed of two catalytic (/') subunits and a non-catalytic β-subunit dimer, whose activity is often abnormally high in cancer cells. The concept that CK2 may be dispensable for cell survival has been challenged by the finding that viable CK2/' knock-out myoblast clones still express small amounts of an N-terminally deleted ' subunit generated during the CRISPR/Cas9 procedure. Here we show that, although the overall CK2 activity of these CK2/Δ' (KO) cells is less than 10% compared to wild-type (WT) cells, the number of phosphosites with the CK2 consensus is comparable to that of WT cells.

Structural similarities between the metacyclic and bloodstream form variant surface glycoproteins of the African trypanosome.

During infection of mammalian hosts, African trypanosomes thwart immunity using antigenic variation of the dense Variant Surface Glycoprotein (VSG) coat, accessing a large repertoire of several thousand genes and pseudogenes, and switching to antigenically distinct copies. The parasite is transferred to mammalian hosts by the tsetse fly. In the salivary glands of the fly, the pathogen adopts the metacyclic form and expresses a limited repertoire of VSG genes specific to that developmental stage.

Balanced activities of Hsp70 and the ubiquitin proteasome system underlie cellular protein homeostasis.

To counteract proteotoxic stress and cellular aging, protein quality control (PQC) systems rely on the refolding, degradation and sequestration of misfolded proteins. In the Hsp70 chaperone system plays a central role in protein refolding, while degradation is predominantly executed by the ubiquitin proteasome system (UPS). The sequestrases Hsp42 and Btn2 deposit misfolded proteins in cytosolic and nuclear inclusions, thereby restricting the accessibility of misfolded proteins to Hsp70 and preventing the exhaustion of limited Hsp70 resources.

Demonstration Laboratory of Industry 4.0 Retrofitting and Operator 4.0 Solutions: Education towards Industry 5.0.

One of the main challenges of Industry 4.0 is how advanced sensors and sensing technologies can be applied through the Internet of Things layers of existing manufacturing. This is the so-called Brownfield Industry 4.

Expression of TRX1 optimizes the antitumor functions of human CAR T cells and confers resistance to a pro-oxidative tumor microenvironment.

Use of chimeric antigen receptor (CAR) T cells to treat B cell lymphoma and leukemia has been remarkably successful. Unfortunately, the therapeutic efficacy of CAR T cells against solid tumors is very limited, with immunosuppression by the pro-oxidative tumor microenvironment (TME) a major contributing factor. High levels of reactive oxygen species are well-tolerated by tumor cells due to their elevated expression of antioxidant proteins; however, this is not the case for T cells, which consequently become hypo-responsive.

[The new approval procedure for clinical trials of medicinal products in the European Union-challenges for the pharmaceutical industry in Germany].

In the European Union (EU), 27 May 2014 was a significant date for clinical trials. Since that day, the EU Clinical Trials Regulation 536/2014 (EU-CTR) officially came into force. The new EU-CTR replaces EU Directive 2001/20/EC and fundamentally reforms the way clinical trials are applied for and conducted in Europe.

NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart.

To adapt to changing hemodynamic demands, regulatory mechanisms modulate actin-myosin-kinetics by calcium-dependent and -independent mechanisms. We investigate the posttranslational modification of human essential myosin light chain (ELC) and identify NIMA-related kinase 9 (NEK9) to interact with ELC. NEK9 is highly expressed in the heart and the interaction with ELC is calcium-dependent.

Goal-oriented possibilistic fuzzy C-Medoid clustering of human mobility patterns-Illustrative application for the Taxicab trips-based enrichment of public transport services.

The discovery of human mobility patterns of cities provides invaluable information for decision-makers who are responsible for redesign of community spaces, traffic, and public transportation systems and building more sustainable cities. The present article proposes a possibilistic fuzzy c-medoid clustering algorithm to study human mobility. The proposed medoid-based clustering approach groups the typical mobility patterns within walking distance to the stations of the public transportation system.

Cotranslational N-degron masking by acetylation promotes proteome stability in plants.

N-terminal protein acetylation (NTA) is a prevalent protein modification essential for viability in animals and plants. The dominant executor of NTA is the ribosome tethered N-acetyltransferase A (NatA) complex. However, the impact of NatA on protein fate is still enigmatic.

High-resolution structures of a thermophilic eukaryotic 80S ribosome reveal atomistic details of translocation.

Ribosomes are complex and highly conserved ribonucleoprotein assemblies catalyzing protein biosynthesis in every organism. Here we present high-resolution cryo-EM structures of the 80S ribosome from a thermophilic fungus in two rotational states, which due to increased 80S stability provide a number of mechanistic details of eukaryotic translation. We identify a universally conserved 'nested base-triple knot' in the 26S rRNA at the polypeptide tunnel exit with a bulged-out nucleotide that likely serves as an adaptable element for nascent chain containment and handover.

Up-regulation of ubiquitin-proteasome activity upon loss of NatA-dependent N-terminal acetylation.

N-terminal acetylation is a prominent protein modification, and inactivation of N-terminal acetyltransferases (NATs) cause protein homeostasis stress. Using multiplexed protein stability profiling with linear ubiquitin fusions as reporters for the activity of the ubiquitin proteasome system, we observed increased ubiquitin proteasome system activity in NatA, but not NatB or NatC mutants. We find several mechanisms contributing to this behavior.