A direct examination of microbial specialized metabolites associated with ocean sediments from Baffin Bay and the Gulf of Maine.

Specialized metabolites produced by microorganisms found in ocean sediments display a wide range of clinically relevant bioactivities, including antimicrobial, anticancer, antiviral, and anti-inflammatory. Due to limitations in our ability to culture many benthic microorganisms under laboratory conditions, their potential to produce bioactive compounds remains underexplored. However, the advent of modern mass spectrometry technologies and data analysis methods for chemical structure prediction has aided in the discovery of such metabolites from complex mixtures.

sp. nov., isolated from Vestrebroggerbreen, a valley glacier in Svalbard, Arctic.

A Gram-stain-negative, rod-shaped bacterial strain designated as 20VBR1 was isolated from a valley glacier (Vestrebroggerbreen) snout ice sample from Ny-Ålesund, Svalbard, Arctic. The colonies were smooth, circular and light creamish on half-strength R2A agar and grew at 10-35 °C (optimum, 20 °C), at pH 6.5-8.

Microbial diversity and associated metabolic potential in the supraglacial habitat of a fast-retreating glacier: a case study of Patsio glacier, North-western Himalaya.

In the present study, we investigated the microbial community composition and their associated metabolic potentials using the 16S rRNA gene (V3-V4) and ITS (ITS1) amplicon sequencing approach in the Patsio glacier. The bacterial community composition was mainly dominated by Bacteroidota (18%-38% of total reads) and Cyanobacteria (9%-30%), along with a rare Candidate phylum Patescibacteria. Ferruginibacter (13%) and Polaromonas (8%) were the most dominant genera identified across the samples known to have potential ecological roles in colonization, driving the functioning of supraglacial habitats.

Roseitranquillus sediminis gen. nov., sp. nov. a novel genus and species of the family Rhodobacteraceae, isolated from sediment of an Arctic fjord.

A Gram-negative, aerobic, non-motile, oxidase-positive, catalase-positive, rod-shaped bacterium, designated strain MCCB 386 was isolated from sediment samples collected from Kongsfjorden, an Arctic fjord. The strain MCCB 386 showed growth at 4-37 °C (optimum 27°C) in the presence of 1-8% NaCl (w/v, optimum 3.5%) and at pH 6.

Genomic insights into the molecular mechanisms of a Pseudomonas strain significant in its survival in Kongsfjorden, an Arctic fjord.

Whole-genome sequence of Pseudomonas sp. Kongs-67 retrieved from Kongsfjorden, an Arctic fjord, has been investigated to understand the molecular machinery required for microbial association and survival in a polar fjord. The genome size of Kongs-67 was 4.

Pseudonocardia cytotoxica sp. nov., a novel actinomycete isolated from an Arctic fjord with potential to produce cytotoxic compound.

Herein we report the isolation of a novel actinomycete, strain MCCB 268, from the sediment sample collected from a high Arctic fjord Kongsfjorden. MCCB 268 showed greater than 97% 16S rRNA gene sequence similarity with those of Pseudonocardia konjuensis LM 157 (98.06%), Pseudonocardia soli NW8-21 (97.

Complete genome sequence and comparative genome analysis of Alcanivorax sp. IO_7, a marine alkane-degrading bacterium isolated from hydrothermally-influenced deep seawater of southwest Indian ridge.

Genome of Alcanivorax sp. IO_7, an alkane degrading deep-sea bacteria isolated from hydrothermally-influenced Southwest Indian Ridge was sequenced and analysed. Genomic data mining revealed gene clusters for degrading n-alkane and cycloalkanes, including biosurfactant production.

Complete genome sequence of Alteromonas pelagimontana 5.12, a marine exopolysaccharide-producing bacterium isolated from hydrothermally influenced deep-sea sediment of eastern Southwest Indian Ridge.

The whole genome of Alteromonas pelagimontana 5.12, a psychrotolerant deep-sea bacterium isolated from the sediment sample of eastern Southwest Indian Ridge, was sequenced and analysed for understanding its metabolic capacities and biosynthesis potential of natural products. The circular genome contained 4.

Bacterial community structure of a glacio-marine system in the Arctic (Ny-Ålesund, Svalbard).

The bacterial community composition of a valley glacier in Svalbard, its pro-glacial channels, and the associated downstream fjord ecosystem was investigated so as to figure out the degree to which downslope transport of microbes from the glacier systems along a hydrological continuum impose an effect on the patterns of diversity in the fjord system. A combination of culture based and high-throughput amplicon sequencing approach was followed which resulted in significant variation (R = 0.873, p = 0.

Alteromonas pelagimontana sp. nov., a marine exopolysaccharide-producing bacterium isolated from the Southwest Indian Ridge.

A novel exopolysaccharide-producing strain, designated as 5.12, was isolated from a sediment sample from the Southwest Indian Ridge, Indian Ocean. The strain was Gram-stain-negative, motile, strictly aerobic, and oxidase- and catalase-positive.

Lipid membrane modulation and pigmentation: A cryoprotection mechanism in Arctic pigmented bacteria.

The present study aims to address the effect of gradual change in temperature (15-4 °C) followed by freeze-thaw on pigmented bacterial strains - Leeuwenhoekiella aequorea, Pseudomonas pelagia, Halomonas boliviensis, Rhodococcus yunnanensis, and Algoriphagus ratkwoskyi, isolated from Kongsfjorden (an Arctic fjord) to understand their survival in present climate change scenario. The total cell count and retrievability of the isolates were not affected despite the variation in temperature. In all the isolates, the saturated fatty acids, particularly stearic and palmitic acid were predominant at higher temperature, while at 4 °C, the unsaturated fatty acids, primarily cis-10-pentadecenoic, palmitoleic, and oleic acid, were major constituents, confirming homeoviscous adaptation.

Streptomyces artemisiae MCCB 248 isolated from Arctic fjord sediments has unique PKS and NRPS biosynthetic genes and produces potential new anticancer natural products.

After screening marine actinomycetes isolated from sediment samples collected from the Arctic fjord Kongsfjorden for potential anticancer activity, an isolate identified as Streptomyces artemisiae MCCB 248 exhibited promising results against the NCI-H460 human lung cancer cell line. H460 cells treated with the ethyl acetate extract of strain MCCB 248 and stained with Hoechst 33342 showed clear signs of apoptosis, including shrinkage of the cell nucleus, DNA fragmentation and chromatin condensation. Further to this treated cells showed indications of early apoptotic cell death, including a significant proportion of Annexin V positive staining and evidence of DNA damage as observed in the TUNEL assay.

Draft Genome Sequence of sp. Strain 5.13, a Highly Stress-Resistant Bacterium Isolated from the Southwest Indian Ridge.

sp. strain 5.13, able to produce biopolymer and exopolysaccharide, was isolated from a sediment sample collected from the Southwest Indian Ridge, Indian Ocean.

Spatio-Temporal Monitoring and Ecological Significance of Retrievable Pelagic Heterotrophic Bacteria in Kongsfjorden, an Arctic Fjord.

Kongsfjorden is a glacial fjord in the Arctic that is influenced by both Atlantic and Arctic water masses. In the present report retrievable heterotrophic bacteria isolated from two distinct zones (outer and inner fjord) of Kongsfjorden was studied during summer to fall of 2012. 16S rRNA gene sequences of the retrievable heterotrophic bacteria corresponded to - (13 phylotypes), - (3 phylotypes), (4 phylotypes) and (2 phylotypes).

Diversity of retrievable heterotrophic bacteria in Kongsfjorden, an Arctic fjord.

The diversity and abundance of retrievable pelagic heterotrophic bacteria in Kongsfjorden, an Arctic fjord, was studied during the summer of 2011 (June, August, and September). Retrievable bacterial load ranged from 10 to 10CFUL in June, while it was 10-10CFUL in August and September. Based on 16S rRNA gene sequence similarities, a higher number of phylotypes was observed during August (22 phylotypes) compared to that during June (6 phylotypes) and September (12 phylotypes).