Synergistic hemolysis between β-lysin-producing Staphylococcus species and Rothia nasimurium in primary cultures of clinical specimens obtained from dogs.

Nonhemolytic isolates of catalase-positive, Gram-positive cocci were observed that produced strong synergistic hemolysis with colonies of Staphylococcus pseudintermedius and Staphylococcus schleiferi subsp. coagulans in primary cultures of clinical specimens from dogs. The isolates appeared similar to nonhemolytic staphylococci but were not identified with conventional biochemical tests or commercial biochemical test kits.

A molecular technique for the detection and differentiation of Demodex mites on cats.

Background: Demodex gatoi causes a pruritic dermatitis in cats. Diagnosis requires the demonstration of mites using superficial skin scrapings or faecal flotation, which can be insensitive.

Hypothesis/objectives: The goal of this study was to develop a molecular method to diagnose D.

PCR amplification and DNA sequencing of Demodex injai from otic secretions of a dog.

Background: The identification of Demodex mites from dogs is usually based on morphology and location. Mites with uncharacteristic features or from unusual locations, hosts or disease manifestations could represent new species not previously described; however, this is difficult to determine based on morphology alone.

Hypothesis/objectives: The goal of this study was to identify and confirm Demodex injai in association with otitis externa in a dog using PCR amplification and DNA sequencing.

Molecular characterization of the L1 gene of papillomaviruses in epithelial lesions of cats and comparative analysis with corresponding gene sequences of human and feline papillomaviruses.

Objective: To characterize the L1 gene of papillomaviruses detected in epithelial lesions of cats and to determine the relationship between those L1 gene nucleotide sequences and known L1 gene sequences of human and feline papillomaviruses.

Sample Population: 10 tissue samples of epithelial lesions from 8 cats.

Procedures: DNA was extracted from tissue samples.

Detection of human papillomavirus DNA in feline premalignant and invasive squamous cell carcinoma.

Squamous cell carcinoma (SCC) is the most common malignant cutaneous and oral neoplasm of cats. Papillomavirus (PV) DNA has been identified in a proportion of feline Bowenoid in situ carcinomas (BISCs), cutaneous SCCs and a single oral SCC, but its exact role in the pathogenesis remains unknown. In humans, it has been suggested that ultraviolet (UV) light and human PV (HPV) may act as cofactors in cutaneous SCC carcinogenesis.