Publications by authors named "Ruoyu Shang"
Dendritic epidermal T cells (DETCs) are a unique subset of γδ T cells that reside predominantly in mouse epidermis; yet, their antitumor functions remain enigmatic. In this study, we report that DETCs mediate potent and exquisitely selective cytotoxicity against diverse tumor types while sparing healthy cells. In vitro, DETCs induced apoptosis in melanoma, hepatoma, colon carcinoma, and lymphoma lines in a dose- and time-dependent manner that required direct cell-cell contact.
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- A study compared a new blood collection technique from mice, called inferior vena cava puncture under continuous cardiac perfusion (IVCP-UCCP), to the traditional cardiac puncture (CP) method.
- The results revealed that IVCP-UCCP collected over twice the amount of usable blood compared to CP, without affecting blood cell composition or viability.
- This improved method allows for more efficient use of limited blood resources in mice, offering benefits for both scientific research and ethical considerations, and could be applied to other small animal models as well.
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- - STING is identified as a vital immune mediator that helps resolve inflammation and aids in skin repair by regulating the movement and function of macrophages during wound healing.
- - Mice lacking STING showed delayed wound healing due to persistent TNF-α+ leukocytes and impaired recruitment of macrophages, highlighting its role in controlling myeloid cell trafficking to wounds.
- - The study reveals that STING enhances macrophage migration through STAT3 activation and regulates chemokine production, suggesting potential therapeutic targets for enhancing wound healing and treating inflammatory diseases.
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- Macrophages play dual roles in skin wound healing, switching from proinflammatory (M1) to anti-inflammatory (M2) states, which is crucial for effective recovery.
- The study identifies fibroblast-derived exosomes as key players that regulate this transition in macrophage activation without direct cell contact, enhancing their responsiveness to changes in the healing environment.
- Administering these exosomes can correct dysfunctional inflammation in chronic wounds, presenting a promising therapeutic avenue for improving healing in inflammatory conditions.
Dysregulated macrophage polarization from pro-inflammatory M1 to anti-inflammatory M2 phenotypes underlies impaired cutaneous wound healing. This study reveals Vγ4 γδ T cells spatiotemporally calibrate macrophage trajectories during skin repair via sophisticated interferon-γ (IFN-γ) conditioning across multiple interconnected tissues. Locally within wound beds, infiltrating Vγ4 γδ T cells directly potentiate M1 activation and suppress M2 polarization thereby prolonging local inflammation.
View Article and Find Full Text PDFBackground: P311, a highly conserved 8 kDa intracellular protein, has recently been reported to play an important role in aggravating hypertrophic scaring by promoting the differentiation and secretion of fibroblasts. Nevertheless, how P311 regulates the differentiation and function of fibroblasts to affect granulation tissue formation remains unclear. In this work, we studied the underlying mechanisms via which P311 affects fibroblasts and promotes acute skin wound repair.
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- The transition from the proinflammatory to prohealing phase is vital for skin wound repair and depends on the balance of M1 and M2 macrophage polarization.
- P311 is crucial in this process as it enhances the function of skin cells and promotes wound closure, but its role in macrophage polarization was previously unclear.
- Deficiency of P311 leads to reduced M2 macrophage polarization, impaired healing processes like angiogenesis and granulation tissue formation, and downregulates the IL-4 receptor, highlighting the importance of P311 in effective wound healing.
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- γδ T cells are essential for the skin's immune defense against damage and infections, differing from conventional αβ T cells in their characteristics.
- In adult mice, key subsets of γδ T cells, like dendritic epidermal T cells (DETCs), Vγ4, and Vγ6, are crucial for skin wound repair through their coordination and interaction.
- The review explores the derivation, development, colonization, and activation of γδ T cells, focusing on their roles in both acute and chronic wound healing to inform research on human epidermal γδ T cells and potential skin treatment options.
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- Efficient re-epithelialization is key for skin wound healing, and the balance of epidermal stem cells (EpSCs) and dendritic epidermal T cells (DETCs) influences this process, although the regulatory mechanism of DETCs on EpSCs is unclear.
- The study utilized normal and Tcrσ knockout mice, applying various analyses (e.g., flow cytometry, immunohistochemistry) to assess how DETCs and their exosomes (Exos) impact EpSCs and wound healing.
- Results indicated that DETCs significantly boost the proportion of specific EpSC markers and enhance proliferation and wound closure through their exosomes, with these
Article Synopsis
- Mesenchymal stem cells (MSCs) are being researched as a therapeutic option for healing injured tissues, with a focus on enhancing their healing abilities.
- The study highlights the importance of neuronal protein 3.1 (P311) in improving MSCs' function, showing that its increased expression boosts healing by reducing inflammation, raising IL10 levels, and promoting significant healing factors like collagen and blood vessel formation.
- The research identifies the mTOR signaling pathway as a key mechanism through which P311 enhances vascular endothelial growth factor (VEGF) production in MSCs, supporting the potential for P311-modified MSCs in more effective skin wound healing treatments.
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) appears to have higher pathogenicity among patients with obesity. Obesity, termed as body mass index greater than 30 kg/m, has now been demonstrated to be important comorbidity for disease severity during coronavirus disease 2019 (COVID-19) pandemic and associated with adverse events. Unraveling mechanisms behind this phenomenon can assist scientists, clinicians, and policymakers in responding appropriately to the COVID-19 pandemic.
View Article and Find Full Text PDFThe epidermis is the outermost layer of skin and the first barrier against invasion. Dendritic epidermal T cells (DETCs) are a subset of γδ T cells and an important component of the epidermal immune microenvironment. DETCs are involved in skin wound healing, malignancy and autoimmune diseases.
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