Publications by authors named "Ruomei Lv"
Cation exchange chromatography (CEX) is commonly used to separate aggregates from monomers during the industrial manufacturing of recombinant proteins. However, the similar isoelectric point of aggregates and monomers makes the stepwise elution CEX an unstable process. In this study, the performance robustness of sodium chloride stepwise elution and cationic buffers (histidine and Bis-Tris) stepwise elution were compared through Monte Carlo simulation.
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- Alpha toxin (CPA) is a harmful substance produced by certain bacteria that causes diseases like yellow lamb disease in sheep and food poisoning in humans, primarily affecting blood cells through hemolysis.
- Recent research indicates that CPA-induced hemolysis relies on the activation of purinergic P2 receptors, revealing that both CPA and another non-pore-forming toxin, β-Hemolysin, use this mechanism.
- The study found that CPA activates specific pathways (PI3K/Akt and MEK1/ERK1) leading to the production of reactive oxygen species, which ultimately contribute to the damage of red blood cells.