[Effects of Effective Fractions of Panax Notoginseng on the Expression of TF and TFPI-2 in Rat En- dometrium with Whole Cycle Exogenous Estrogen Intervention].

Objective To observe the effects of notoginsenoside, Panax notoginseng flavonoids (PNF) , Panax notoginseng acid (PNA) , and their mixtures on the expressions of tissue factor (TF) and tissue factor pathway inhibitor 2 (TFPI-2) in rat endometriaum with whole cycle exogenous estrogen inter- vention. Methods Totally 160 female impuberty rats were randomly divided into the blank group (n =40) and the model group (n =120). Rats in the blank group were administered with normal saline by gastroga- vage for 3 weeks, while those in the model group were administered with estradiol valerate suspension.

[Effect of total flavones from Cuscuta chinensis on expression of Th type-1/Th type-2 cytokines, serum P and PR in abortion rats model].

Objective: To study the effect of TFCC on expression of Th type-1/Th type-2 cytokines and PR in rats abortion model, and observe the changes of serum P and PRL.

Methods: Early abortion rat model was established by Bromocriptine at 6-8 gestation day, the model rats were treated with high and low dosage of TFCC and the progesterone as the positive control. Progesterone and prolactin levels in the serum were measured by radioimmunoassay, the protein expression of PR was inspected by immunohistochemical, RT-PCR was applied to determine the expression of Thl/Th2 mRNA on deciduas.

[Effect of ingredients of Astragalus-Salvia compound on vascular endothelial cell in placenta and vascular endothelial growth factor mRNA expression in trophocyte in pregnant rats with inhibited nitric oxide synthesis].

Objective: To investigate the molecular mechanisms of effective ingredients of Astragalus-Salvia compound (ASC) in increasing placental blood supply, to provide thought for establishing an effective prevention and treatment of insufficient placental blood supply caused complications of pregnancy.

Methods: The effective ingredients of ASC was isolated and abstracted and the rat model of inhibited nitric oxide synthesis was established by using L-Arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor. The mRNA expression of vascular endothelial growth factor (VEGF) in placental trophocyte and primordial micrangial endothelial cell was determined by using in situ hybridization at 12th day of pregnancy.