[Effects of Astragalin on apoptosis of undifferentiated gastric cancer cells].

Objective: To investigate the effects and related molecular mechanisms of Astragalin on undifferentiated gastric cancer cell HGC-27.

Methods: Astragalin was used to treat HGC-27 cells, the cell proliferation activity was detected by CCK-8 method, the cell morphology was observed under inverted microscope, hoechst 33342 and JC-1 staining were used to observe the changes of nucleus formation and mitochondrial membrane potential, the cell cycle and apoptosis rate were detected by flow cytometry, the reverse transcription level of the gene was analyzed by the second-generation sequencer.

Results: Astragalin inhibited the proliferation of HGC-27 significantly (＜0.

[The effects of shikonin on liver cancer cells SMMC-7721 apoptosis and its mechanism].

To investigate the effects and molecular mechanisms of shikonin on liver cancer SMMC-772 cells. SMMC-7721 cells were treated with shikonin at the concentrations of 0, 5, 20, 80 and 320 ng/ml for 0, 24, 48 and 72 h respectively. The proliferative activity of the cells was detected by CCK8 assay.