Publications by authors named "Ruo-Cheng Xia"
Objectives: To establish a rapid, accurate, and sensitive multiplex PCR detection method for the simultaneous identification of the six common edible meats (beef, lamp, chicken, pork, goose, duck), and to evaluate its application value in meat adulteration identification.
Methods: Based on complete mitochondrial genomic sequences of six species in the GenBank database, DNA sequences (cattle：; sheep：; chickens：; pig：; goose：; duck：) with intra-species conservation and inter-species specificity were screened, and species-specific primers were designed to construct a multiplex PCR detection system that can simultaneously detect the meat of six common species. The species specificity, sensitivity and reproducibility of the system were studied, and the simulated mixture sample detection was performed.
Application of SNaPshot Technology in Semen-Specific cSNP Genetic Marker.
Fa Yi Xue Za Zhi
October 2023
Objectives: To explore the feasibility of genetic marker detection of semen-specific coding region single nucleotide polymorphism (cSNP) based on SNaPshot technology in semen stains and mixed body fluid identification.
Methods: Genomic DNA (gDNA) and total RNA were extracted from 16 semen stains and 11 mixtures composed of semen and venous blood, and the total RNA was reverse transcribed into complementary DNA (cDNA). The cSNP genetic markers were screened on the validated semen-specific mRNA coding genes.
Application of SifaInDel 45plex System in the Han and Mongolian Populations.
Fa Yi Xue Za Zhi
December 2022
Article Synopsis
- The study aimed to analyze the genetic variation of InDel loci using the SifaInDel 45plex system among the Han population in Jiangsu Province and the Mongolian population in Inner Mongolia, assessing its utility in forensic applications.
- Blood samples from 398 unrelated individuals were genotyped to calculate allele frequencies and examine genetic distances between the two populations and other reference groups, ultimately constructing phylogenetic trees.
- Findings revealed strong genetic diversity in the studied populations, demonstrating that the Jiangsu Han and Inner Mongolia Mongolian groups are closely related genetically, and the InDel markers are highly effective for forensic purposes.
Article Synopsis
- The study aimed to analyze genetic polymorphism and parameters of 16 X-STR loci in the Xinjiang Uygur population using a DNA identification system.
- A total of 502 unrelated individuals were examined, revealing 67 detected alleles with high cumulative discrimination power for individual and paternity identification.
- The Uygur population showed genetic closeness to the Kazakh population and a greater genetic distance from the Han population, highlighting the X-STR loci's effectiveness for genetic studies.
Objectives: To construct a STR loci multiplex amplification system and to evaluate its application value by testing the technical performance.
Methods: The published STR loci data were reviewed and analyzed to select the STR loci and sex identification loci that could be used for individual identification and genetic identification. The fluorescent labeling primers were designed to construct the multiplex amplification system.
Objectives: To evaluate the ability of the ForenSeq DNA Signature Prep kit (ForenSeq kit) in analyzing the sequence information of STRs in Zhejiang She ethnic group and its forensic application efficacy.
Methods: A total of 50 Zhejiang She ethnic group samples were sequenced with the ForenSeq kit on the MiSeq FGx platform. The data was analyzed using ForenSeq universal analysis software to obtain the motif structure and flank regions of the 58 STRs, then compared with PCR-CE typing results to test the consistency.