Lactoferrin Induces Erythropoietin Synthesis and Rescues Cognitive Functions in the Offspring of Rats Subjected to Prenatal Hypoxia.

The protective effects of recombinant human lactoferrin rhLF (branded "CAPRABEL™") on the cognitive functions of rat offspring subjected to prenatal hypoxia (7% O, 3 h, 14th day of gestation) have been analyzed. About 90% of rhLF in CAPRABEL was iron-free (apo-LF). Rat dams received several injections of 10 mg of CAPRABEL during either gestation (before and after the hypoxic attack) or lactation.

Proatherogenic modification of LDL by surface-bound myeloperoxidase.

One of the factors promoting oxidative/halogenating modification of low-density lipoproteins (LDL) is myeloperoxidase (MPO). We have shown previously that MPO binds to the LDL surfaces. The LDL-MPO complex is uncoupled in the presence of peptide EQIQDDCTGDED that corresponds to a fragment of apoB-100 (445-456).

Study of interaction of ceruloplasmin with serprocidins.

This paper describes formation of complexes of ceruloplasmin (CP) with such proteins of the serprocidin family as azurocidin (CAP37), neutrophilic elastase (NE), cathepsin G (CG), and proteinase 3 (PR3). We present evidence that serprocidins form complexes with CP at a molar ratio 1 : 1. Phenylmethylsulfonyl fluoride, a serine protease inhibitor, did not prevent the interaction of serprocidins with CP in the course of SDS-free disc electrophoresis.

[Familial Alzheimer's disease mutations in the presenilin 1 gene reduce cell-cell adhesion in transfected fibroblasts].

Experimental evidence has been obtained that mutations in the presenilin 1 (PS1) gene in familial Alzheimer's disease can lead to the disturbance of cell adhesion in model cell cultures. It was shown that, in L fibroblasts of mice with stable expression of GFP-PS1 cDNA containing G209V or E319G mutations, cell-cell interactions and the accumulation of GFP-PS1 cDNA in intercellular contacts are disturbed. Similar results were obtained in transfected human epithelial Hep2 cells.

[Degeneration of growth cones in a culture of embryonic neurons of mouse with presenilin 1 gene knockout].

A comparative study of growth cone morphology in cultured embryonic neurons derived from wild type PS 1(+/+) and knockout PS 1(-/-) mice has been performed. Growth cones from wild type PS 1(+/+) mice were well spread and usually formed radially continuous and regular lamellar extensions with numerous filopodia. In contrast, most growth cones from knockout PS 1(-/-) mice exhibited small lamellar extensions, short filopodia, and pure adhesion.

[The organization of the rehabilitation of children with the after-effects of perinatal pathology of nervous system in the conditions of day-stay hospital].

The new organizational pattern of hospital-substituting technology--the day-stay hospital for the rehabilitation of the children suffering from chronic somatic diseases and after-effects of perinatal lesion of the central nervous system. This approach gives an opportunity to implement a relevant and up-to-date neuro-somatic approach using the potential of medical specialists from the pediatric polyclinic's staff The priority is given to the non-pharmaceutical techniques of rehabilitation to permitting to come about the higher results even without expensive pharmaceuticals.

Identification of leukocyte cationic proteins that interact with ceruloplasmin.

Proteins from leukocytes were investigated for their ability to interact with ceruloplasmin (Cp), a copper-containing glycoprotein of human plasma. Extract from leukocytes was subjected to affinity chromatography on Cp-Sepharose, after which proteins were eluted from the resin with 0.5 M NaCl in Tris-HCl, pH 7.

[Expression of presenilin 1 on the cell surface in motile polarized cells].

Most cases of familial early-onset Alzheimer's disease are caused by mutations in the presenilin 1 (PS1) gene. However, the cellular functions of PS1 are not yet completely understood. We showed that endogenous PS1 and the adhesion protein CD44 are redistributed on the surface of cell projections (lamellipodia) in polarized T- lymphocytes (Jurkat cells) after the adhesion to a collagen matrix.

Identification and isolation from breast milk of ceruloplasmin-lactoferrin complex.

The presence of a complex of the copper-containing protein ceruloplasmin (Cp) with lactoferrin (Lf) in breast milk (BM) is shown for the first time. In SDS-free polyacrylamide gel electrophoresis (PAGE), electrophoretic mobility of Cp in BM is lower than that of plasma Cp, coinciding with the mobility of the complex obtained upon mixing purified Cp and Lf. Affinity chromatography of delipidated BM on Cp-Sepharose resulted in retention of Lf.

[Protein of Escherichia coli interacting specifically with human low density lipoproteins].

Escherichia coli 48 kDa protein interacting specifically with human low-density lipoproteins is described. The dissociation constant of this highly specific interaction was found to be equal to 4 mkg LDL per 1 ml or 7.3 x 10 M, which is comparable with the dissociation constant of the complex formed by LDL and human LDL receptor.

[Quantitative determination of phenol in allergens and vaccines by gas liquid chromatography].

A method for measuring phenol by gas liquid chromatography has been developed. Metrological characteristics of the method were evaluated: similarity (V no more than 1.62%), reproducibility (V no more than 1.

Expression of cDNA fragment encoding ligand-binding domain of human low density lipoprotein receptor in Escherichia coli cells.

To study structure-function relationships in low density lipoprotein receptor (LDLR), a key protein in human cholesterol metabolism, it is reasonable to operate with separate protein domains. To obtain highly purified functionally active LDLR ligand-binding domain, we have cloned the corresponding LDLR cDNA fragment in two expression plasmid vectors of Escherichia coli. We have developed methods to purify fusion and practically individual recombinant proteins and characterized the obtained products biochemically.

[The modelling of the genetic correction of familial hypercholesterolemia by creating cellular clones stably expressing the human low-density lipoprotein receptor].

The stable expression of human low-density lipoprotein receptor (rLDL) was studied in Chinese hamster ovary (CHO) cells transformed by the pMSVL recombinant plasmid. This plasmid contains full-length rLDL cDNA and hybrid promoter consisting of regulatory elements from early SV-40 promoter and glucocorticoid responsive enhancer of Moloney sarcoma virus. The expression of rLDL cDNA in transformed cells was demonstrated using RNA-cDNA blot hybridization, PCR analysis, and immunofluorescent analysis.