Early Use of Blood Purification in Severe Epstein-Barr Virus-Associated Hemophagocytic Syndrome.

Epstein-Barr virus-associated hemophagocytic lymphohistiocytosis (EBV-HLH) is a common type of hemophagocytic lymphohistiocytosis (HLH) that exhibits high rates of morbidity and fatalities. Multiorgan failure caused by Epstein-Barr virus (EBV)-induced hypercytokinemia is one of the main reasons for early deaths. Blood purification techniques have been successfully applied in previously treated hypercytokinemia.

Identification of HS/NO-donating artemisinin derivatives as potential antileukemic agents.

Three HS/NO-donating artemisinin derivatives were designed and synthesized. Their antiproliferative activities were evaluated against human acute myeloid leukemia (AML) cell lines of K562 and K562/ADR and human normal liver cells of LO2. Biological evaluation indicated that NO-donating compound 10c exhibited the most potent cytotoxicity against leukemia cells, similar to the bioactivity of clinical drug of homoharringtonine, but showed less toxicity than homoharringtonine against LO2 cells.

[Effect of Silencing and Overexpression of LNK Gene on STAT3 Expression in THP-1 Cells].

Objective: To investigate the effect of LNK gene silencing and overexpression on the expression of STAT3 gene in human monocytic leukemia cells (THP-1).

Methods: THP-1 cells were cultured, and the lentivirus was used as a vector to silence and overexpres the LNK gene stably. After transfection for 72 hours, the GFP expression levels were observed by inverted fluorescence microscopy.

[EPO and EPOR Expression in Patients with Acute Leukemia and Its Clinical Significance].

Objective: To investigate the expression of erythropoietin (EPO) and erythropoietin receptor (EPOR) in patients with acute leukemia (AL) and its clinical significance.

Methods: The levels of EPO and EPOR in plasma were determined by ELISA kit. mRNA expression levels of EPO and EPOR were determined by RT-RCR.

[Effect of Silencing LNK Gene on the Expression of EPO and EPOR in THP-1 Cells].

Objective: To investigate the effect of silencing LNK gene on the expression of EPO and EPOR in acute myeloid leukemia cells (THP-1).

Methods: THP-1 cells were cultured. The lentivirus was used as a vector to silence the LNK gene stably.

Severe Thalassemia Caused by Hb Zunyi [β147(HC3)Stop→Gln; : c.442T>C)] on the β-Globin Gene.

Hemoglobinopathies are caused by genetic defects on the globin genes. To date, more than 900 β-globin variants have been recorded worldwide. These gene alterations often cause either a decrease in β-globin synthesis or completely block synthesis, leading to a hemoglobinopathy.

[Expression and Clinical Significance of STAT3 Genes in Patients with Acute Myeloid Leukemia].

Objective: To investigate the expression of STAT3 gene in patients with acute myeloid leukemia and its correlation with clinical characteristics.

Methods: The real-time quantitative RT-PCR was used to detect the level of STAT3 mRNA in bone marrow samples from 38 newly diagnosed patients with acute myeloid leukemia(AML), and its relevance with clinical characteristics and prognosis were statistically analyzed. Western blot was employed to detect the STAT3 protein level in AML patients.

[Change of G6PD Activity in Children with Acute Leukemia and Its Clinical Significance].

Objective: To explore the change of G6PD activity in children with acute leukemia（AL）and its correlation with the clinical characteristics.

Methods: The G6PD activity in peripheral blood samples from 74 children disagnosed as AL (50 cases of ALL, and 24 cases of AML) was detected by Zinkham method recommended by WHO in 1967, and its relevance with clinical indicators was statistically analyzed. The peripheral blood samples of 70 healthy children were used as the controls.

[Artesunate Suppresses Cell Proliferation of THP-1 Cells by Inducing Apoptosis].

Objective: To investigate whether Artesunate(ART) can inhibit the proliferation of THP-1 cells and to explore the potential mechanism of its anti-leukemia effect.

Methods: THP-1 cells were treated with 5 concentrations of Artesunate for 24 h, 48 h or 72 h. The viability of cells was detected with CCK-8 assay, apoptosis was assessed by using flow cytometry, and the STAT3, Caspase3 and Caspase8 protein levels were measured with Western blot .

[Expression of LNK Genes in Patients with Acute Leukemia and Its Clinical Significance].

Objective: To investigate the expression of LNK gene in patients with acute leukemia (AL) and its correlation with the clinical characteristics.

Methods: Real-time quantitative RT-PCR was used to detect the level of LNK mRNA in bone marrow samples from 80 patients diagnosed as AL(42 cases of ALL, and 38 cases of AML), and its relevance with clinical indicators was statistically analyzed. Western blot was used to detect the expression of LNK protein.

[Variation and Clinical Significance of LNK Gene in Essential Thrombocytosis].

Objective: To explore the mutation and single nucleotide polymorphism(SNP) of LNK gene in the patients with essential thrombocytosis (ET), and to analyze the relationship between LNK gene variation and the occurrence of ET.

Methods: JAK2V617F mutation was identified by allele-specific PCR. The whole exon of LNK gene was amplified by PCR.

β‑thalassemia caused by compound heterozygous mutations and cured by bone marrow transplantation: A case report.

In the present study, a rare familial case of severe thalassemia with compound spontaneous mutations is reported. A 2.5‑year‑old boy, who suffered from severe anemia with yellowish skin, enlarged liver and spleen, was provided with a blood transfusion every 20 days to maintain hemoglobin levels between 90 and 100 g/l.

[Variation of LNK Gene in Chronic Myeloid Leukemia].

Objective: To compare the mutation and single nucleotide polymorphism (SNP) of LNK gene between chronic myeloid leukemia(CML) and control groups, and to explore the relationship between LNK gene variation and the occurrence of CML.

Methods: A total of 36 patients with CML were selected, 46 healthy persons were used as normal controls. DNA was extracted from bone marrow and peripheral blood, BCR/ABL1 fusion gene was detected by Q-PCR.