Itaconic acid/cellulose-based hydrogels with fire-resistant and anti-freezing properties via vat photopolymerization 3D printing.

Hydrogel-born materials have garnered significant interest due to their inherent flame retardant properties and eco-friendly characteristics. In light of the diminishing petroleum reserves and the escalating environmental challenges, there is an urgent impetus to exploit high-value applications of naturally occurring resources and to advance research in sustainable manufacturing technologies. In this vein, we describe an innovative and sustainable methodology for the development and production of flame-retardant hydrogels.

poxtA amplification and mutations in 23S rRNA confer enhanced linezolid resistance in Enterococcus faecalis.

Objectives: This study aimed to explore the evolutionary patterns and resistance mechanisms of an Enterococcus faecalis strain harbouring poxtA under linezolid exposure.

Methods: A poxtA-carrying E. faecalis electrotransformant DJH702 with a linezolid minimum inhibitory concentration of 4 mg/L was exposed to increasing concentrations of linezolid (8-64 mg/L).

Tandem amplification of a plasmid-borne tet(A) variant gene confers tigecycline resistance in Escherichia coli.

Objectives: To elucidate the mechanism of tigecycline resistance in Escherichia coli that is mediated by the tet(A) variant gene.

Methods: E. coli strain 573 carried a plasmid-borne tet(A) variant gene, tentatively designated tet(A)TIG, that conferred decreased tigecycline susceptibility (MIC 0.

3D Printed All-Natural Hydrogels: Flame-Retardant Materials Toward Attaining Green Sustainability.

Biomass-based hydrogel is a promising flame-retardant material and has a high potential for applications in transportation, aerospace, building and electrical engineering, and electronics. However, rapid vat photopolymerization (VP) 3D printing of biomass-based hydrogels, especially that of all-natural ones, is still rare. Herein, a new class of VP 3D-printed hydrogels with strong covalent networks, fabricating using fully biomass materials and a commercial liquid crystal display (LCD) printer assembled with low-intensity visible light is presented.

Emergence of high-level tigecycline resistance due to the amplification of a tet(A) gene variant in clinical carbapenem-resistant Klebsiella pneumoniae.

Objective: To investigate the prevalence of a tet(A) gene variant and its role in developing high-level tigecycline resistance among carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical isolates.

Methods: The mechanism of high-level tigecycline resistance in CRKP mediated by a tet(A) variant was explored by induction experiments, antimicrobial susceptibility testing, whole-genome sequencing and bioinformatics analysis. The amplification and overexpression of the tet(A) variant were measured by the determination of sequencing depth, gene copy numbers, and qRT-PCR.

Emergence of lnu(C) variant conferring lincomycin resistance in Campylobacter coli of chicken origin.

Lincomycin is widely used in respiratory and gastrointestinal infection in veterinary medicine and food animal production. Campylobacter members are vital foodborne pathogens causing campylobacteriosis, and the resistance to lincosamides is seldom reported. To date, only the rRNA methyltransferase Erm(B) has been confirmed to be associated with lincosamides resistance in Campylobacter.

Identification of Novel (X3) Variants Resistant To Tigecycline in Acinetobacter Species.

The emergence of the (X) gene is a severe challenge to global public health security, as clinical tigecycline resistance shows a rapidly rising trend. In this research, we identified two tigecycline-resistant Acinetobacter sp. strains containing seven novel (X3) variants recovered from fecal samples from Chinese farms.

Characterization of a genomic Island carrying the (X4) gene in porcine co-harboring plasmid-borne and genes.

Tigecycline and carbapenems are last-resort antimicrobial agents to treat serious infections caused by multi-drug resistant bacterial pathogens. However, the co-occurrence of tigecycline and carbapenem resistance determinants challenges the clinical efficacy of these antimicrobial agents. In this study, we report the co-existence of (X4), and genes in the porcine isolate 19110F47.

Studies on the Transmission of a Tigecycline Resistance-Mediating (A) Gene Variant from Enterobacter hormaechei via a Two-Step Recombination Process.

To investigate the contribution of a (A) variant to tigecycline resistance in Enterobacter hormaechei and the recombination events that occurred during transmission of this variant. MICs were determined by broth microdilution. G17 was characterized by PCR, transfer assay, S1-PFGE, Southern blot hybridization, and WGS analysis.

Molecular Characterization of an IncFII Plasmid Co-harboring and (A) Variant in a Clinical Isolate.

Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both and (A) variant in clinical KP-1572. MIC results showed that KP-1572 was resistant to a wide range of antimicrobials.

A novel multiresistance gene cluster located on a plasmid-borne transposon in Listeria monocytogenes.

Objectives: To identify the genetic context and the transferability of the multiresistance gene lsa(E) in Listeria monocytogenes.

Methods: MICs were determined by broth microdilution. Transferability of lsa(E) was investigated by conjugation, electrotransformation and natural transformation.