Development of konjac glucomannan-based active-intelligent emulsion films loaded with different curcumin-metal chelates: Stability, antioxidant, fresh-keeping and freshness detection properties.

The aim of this study was to develop konjac glucomannan (KGM)-based active-intelligent emulsion films loaded with different curcumin-metal chelates, where six types of films were prepared and their corresponding properties were investigated. The FTIR and XRD results showed that curcumin chelated with metal ions successfully. Moreover, curcumin-Ca chelate had the best thermal stability and antioxidant activity with the DPPH and ABTS radical-scavenging activity values of 38.

Construction and antibacterial activities of walnut green husk polysaccharide based silver nanoparticles (AgNPs).

In this paper, the size-controllable nano‑silver particles (AgNPs) were synthesized from walnut green husk polysaccharide, and its cytotoxicity and antibacterial activity were evaluated. Firstly, acidic polysaccharide WGHP2 was extracted from walnut green husk, and then the silver ion in AgNO was reduced in WGHP2 aqueous solution using NaBH, so as to synthesize the nano‑silver composite. The nano‑silver composite was characterized by transmission electron microscope, Fourier infrared spectroscopy, ultraviolet-visible spectrometer, scanning electron microscope, inductively coupled plasma mass spectrometry and X-ray photoelectron spectroscopy.

Bacterial cellulose nanofibers used as nanofillers improved the fresh-keeping performance of gelatin-based edible coating for fresh-cut apples.

In this study, bacterial cellulose nanofibers (BCNs) (0%, 1%, 2%, and 3%) were used as nanofillers to prepare gelatin-based edible films, and their physical properties and fresh-keeping performance were investigated. The microstructure observation showed that the BCNs were well dispersed in the gelatin-based edible films and the surface roughness of the films increased with the increase of BCNs content. X-ray diffraction and thermogravimetric analysis showed that the crystallinity and thermal stability of the film were significantly increased with the increase of BCNs.

Promoting adsorption performance and mechanical strength in composite porous gel film.

Starch is widely used to prepare biodegradable films due to its superior biocompatibility, low immunogenicity, and renewability. In this work, a novel K/carrageenan porous-starch/casein gel film with high oil absorption was prepared using modified porous starch. Optimal gel stability and uniformity were obtained when adding 10 mg/mL k-carrageenan and 2 mg/mL K to 2 mg/mL microgels, with significantly reduced crystallinity and elasticity and increased tensile strength.

Characterization of structural, functional and antioxidant properties and amino acid composition of pepsin-derived glutelin-1 hydrolysate from walnut processing by-products.

Glutelin-1 of defatted walnut meal protein (DWPG-1) was modified by pepsin enzymatic hydrolysis to improve its functional properties and antioxidant activities. The amino acid composition, structural characteristics, physicochemical and functional properties as well as antioxidant activities of the hydrolysate were compared with those of unmodified DWPG-1. The analysis of X-ray diffraction patterns, surface microstructure and particle size distribution indicated that enzymatic hydrolysis changed the structures of DWPG-1.

Novel Angiotensin-Converting Enzyme Inhibitory Peptides Identified from Walnut Glutelin-1 Hydrolysates: Molecular Interaction, Stability, and Antihypertensive Effects.

In recent years, angiotensin-converting enzyme (ACE) inhibitory peptide has become a research hotspot because of its essential role in maintaining human blood pressure balance. In this study, two novel ACE inhibitory peptides of Val-Glu-Arg-Gly-Arg-Arg-lle-Thr-Ser-Val (Valine-Glutamate-Arginine-Glycine-Arginine-Arginine-Isoleucine-Threonine-Serine-Valine, VERGRRITSV) and Phe-Val-Ile-Glu-Pro-Asn-Ile-Thr-Pro-Ala (Phenylalanine-Valine-Isoleucine-Glutamate-Proline-Asparagine-Isoleucine-Threonine-Proline-Alanine, FVIEPNITPA) were isolated and purified from defatted walnut meal hydrolysates through a series of preparation processes including ultrafiltration, Sephadex G-15 gel chromatography, and reverse high performance liquid chromatography (RP-HPLC). Both peptides showed high ACE inhibitory activities.

Effect of Juglone against Planktonic Growth and Biofilm Formation.

() is a common pathogen causing plant diseases. Limoli proved that its strong pathogenicity is closely related to biofilm state. As a natural bacteriostatic agent with broad-spectrum bactericidal properties, juglone can be used as a substitute for synthetic bacteriostatic agents.

Rapid Detection of Dimethoate in Soybean Samples by Microfluidic Paper Chips Based on Oil-Soluble CdSe Quantum Dots.

Given the imperative of monitoring organophosphorus pesticides (OPs) residues in the ecosystem, here a novel, facile and sensitive fluorescence sensor is presented for the rapid detection of dimethoate. In this work, surface molecularly imprinted polymer (SMIP) and microfluidic technology had been introduced to enhance the selectivity and portability of the described methodology. Oil-soluble CdSe quantum dots (QDs) synthesized in a green way were used as fluorescent material for the selective detection of dimethoate on the basis of static quenching and photoinduced electron transfer mechanism.

Load phycocyanin to achieve in vivo imaging of casein-porous starch microgels induced by ultra-high-pressure homogenization.

Traditional bioactive substances are often limited in practical application due to their poor stability and low solubility. Therefore, it is imperative to develop biocompatible high loading microgel carriers. In this study, a novel type of casein-porous starch microgel was prepared under ultra-high-pressure homogenization, by using porous starch with the honeycomb three-dimensional network porous structure.

Juglone Inactivates through Cell Membrane Damage, Biofilm Blockage, and Inhibition of Gene Expression.

Due to the strong drug resistance of (), the inhibition effects of conventional disinfectants and antibiotics are not obvious. Juglone extracted from discarded walnut husk, as a kind of plant-derived antimicrobial agent, has the advantages of naturalness, high efficiency, and low residue, with a potential role in the inhibition of . This study elucidated the inhibitory effect of juglone on the growth of plankton and the formation of biofilm.

Effect of Walnut Meal Peptides on Hyperlipidemia and Hepatic Lipid Metabolism in Rats Fed a High-Fat Diet.

As a natural active substance that can effectively improve blood lipid balance in the body, hypolipidemic active peptides have attracted the attention of scholars. In this study, the effect of walnut meal peptides (WMP) on lipid metabolism was investigated in rats fed a high-fat diet (HFD). The experimental results show that feeding walnut meal peptides counteracted the high-fat diet-induced increase in body, liver and epididymal fat weight, and reduce the serum concentrations of total cholesterol, triglycerides, and LDL-cholesterol and hepatic cholesterol and triglyceride content.

Walnut green husk polysaccharides prevent obesity, chronic inflammatory responses, nonalcoholic fatty liver disease and colonic tissue damage in high-fat diet fed rats.

High-fat (HF) diets cause obesity, gut microbial dysbiosis and associated disorders and inflammatory bowel disease (IBD) due to increased intestinal permeability, which is an important reason for chronic inflammation and oxidative stress. This study was to investigate the effects and mechanism by which walnut green husk polysaccharides (WGHP) prevents obesity, oxidative stress, inflammation, liver and colon damage in HF diet induced rats. We found that WGHP alleviated HF-induced abnormal weight gain, disordered lipid metabolism, inflammation, oxidative stress, colonic tissue injury and up-regulate the expression level of colonic tight junction protein in the rats.

Boronate affinity material-based sensors for recognition and detection of glycoproteins.

Glycoproteins are closely linked to the occurrence and development of many diseases. Therefore, it is of great importance to develop highly selective, sensitive, efficient detection methods for glycoproteins. To overcome the problems with traditional detections methods, such as mass spectrometry, chromatography-mass spectrometry, and enzyme-linked immunosorbent assay, boronate affinity material (BAM)-based sensors have developed rapidly for the specific recognition and detection of glycoproteins because of the advantages of pH-controlled binding/release, reversibility of the reaction, high specificity, and high selectivity, showing their wide application prospects.

The protective effects of walnut green husk polysaccharide on liver injury, vascular endothelial dysfunction and disorder of gut microbiota in high fructose-induced mice.

This study aimed to investigate the protective effects of walnut green husk polysaccharide (WGHP) on liver injury, vascular endothelial dysfunction and disorder of gut microbiota in mice induced by high fructose (HF) diet. The chemical analysis results show that the walnut green husk polysaccharide is a low molecular weight acidic heteropolysaccharide, composed mainly of glucuronic acid, arabinose and galactose. Biochemical analysis showed that WGHP significantly improved glucose metabolism and lipid metabolism and decreased oxidative stress in HF-diet induced obesity mice.

New insights into the alleviating role of Melaleuca alternifolia oil on metabolites pathway disorder of grapes caused by Aspergillus niger, verified by corresponding key genes expression.

Grape berries are susceptible to Aspergillus niger (A. niger) infection during storage, leading to a significant reduction in its nutritional quality. However, most alternations in nutrient contents and related gene expression during fungal infection or treated with antimycotics remain unexplored.

Enhancing the antitumor activity of tea polyphenols encapsulated in biodegradable nanogels by macromolecular self-assembly.

Nanogels (NGs) with desirable stability have emerged as a promising platform for biomedical applications. Herein, a convenient approach was developed to encapsulate and protect tea polyphenols (TPs) by macromolecular self-assembly of lysozyme (Ly) and carboxymethyl cellulose (CMC) through a heating treatment. Biodegradable Ly-CMC NGs were formed on the basis of molecules driven by electrostatic interaction and hydrophobic forces.

Influence of modified atmosphere treatment on post-harvest reactive oxygen metabolism of pomegranate peels.

Modified atmosphere storage can regulate the reactive oxygen metabolism of fruits and vegetables, reduce the accumulation of hazardous free radicals, and mitigate the peroxidation degree of fruit membrane lipids. In this study, different gas matching ratios were adopted for the modified atmosphere treatment of pomegranate fruits. Up to 120 d of storage, compared with the control treatment, the HO and malonaldehyde (MDA) contents in treatment 2 decreased by 8.

Apoptosis triggered by isoquercitrin in bladder cancer cells by activating the AMPK-activated protein kinase pathway.

Cancer cells are well known to require a constant supply of protein, lipid, RNA, and DNA via altered metabolism for accelerated cell proliferation. Targeting metabolic pathways is, therefore, a promising therapeutic strategy for cancers. Isoquercitrin (ISO) is widely distributed in dietary and medicinal plants and displays selective cytotoxicity to cancer cells, primarily by inducing apoptosis and cell cycle arrest.

Phenolic composition, DNA damage protective activity and hepatoprotective effect of free phenolic extract from Sphallerocarpus gracilis seeds.

The phenolic composition of the free phenolic extract from Sphallerocarpus gracilis seeds was analyzed by HPLC-MS and predominant compounds were chlorogenic acid, di-caffeoylquinic acid glucoside and luteolin-7-O-glucoside. The free phenolic extract was evaluated for DNA damage protective activity induced by ROO and OH radicals and hepatoprotective effect in vivo and in vitro. Results revealed that the free phenolic extract exhibited significant protective activity against both ROO and OH radical-induced DNA damage and the phenolic extract exerted more potent inhibitory activity against OH radical-induced damage than against that induced by ROO radicals.