A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications.

Developing highly sensitive and selective methods for Cu detection in living systems is of great significance in clinical copper-related disease diagnosis. In this work, a near infrared (NIR) fluorescent probe, CySBH, with a salicylaldehyde benzoyl hydrazone group as a selective and sensitive receptor for Cu was designed and synthesized. The specific coordination of the salicylaldehyde benzoyl hydrazone group in CySBH with Cu can induce a distinct quench of the fluorescence intensity, allowing for real-time tracking of Cu.

A colorimetric method for screening α-glucosidase inhibitors from flavonoids using 3,3',5,5'-tetramethylbenzidine as a chromogenic probe.

A facile and novel colorimetric method for screening of α-glucosidase inhibitors (AGIs) from flavonoids using 3,3',5,5'-tetramethylbenzidine (TMB) as a chromogenic probe is proposed. This method is based on the colorimetric detection of ascorbic acid (AA) through the TMB oxidation reaction catalyzed by horseradish peroxidase (HRP) in the presence of hydrogen peroxide (HO). In the TMB/HO/HRP system, HRP catalyzes the oxidation of HO to ‧OH radical which oxidizes TMB to blue-colored oxidized TMB (oxTMB).

"Recent advances on support materials for lipase immobilization and applicability as biocatalysts in inhibitors screening methods"-A review.

With a substantial demand for new anti-obesity drugs for the treatment of obesity, screening lipase inhibitors from natural products has become a popular approach toward drug discovery. Due to the significant advantages of excellent reusability, stability and endurance in extreme pH and temperature conditions, lipase immobilization has been employed as a promising strategy to screen lipase inhibitors. Support is a key factor in the process of enzyme immobilization used to provide excellent biocompatibility, stable physical and chemical properties and abundant binding sites for enzymes.

An immobilization enzyme for screening lipase inhibitors from Tibetan medicines.

With the increasing demand for lipase inhibitors and new drugs used in the clinical treatment of obesity, it is of great significance to screen lipase inhibitors from traditional Chinese medicines (TCMs) via capillary electrophoresis. In this work, FeO@TiO nanoparticles was fabricated by solvothermal method and employed as an improved magnetic support to immobilize lipase through electrostatic interaction. By the method of transmission electron microscopy, fourier transform infrared spectroscopy and X-ray diffraction, the magnetic nanoparticles were characterized.

A fluorescent molecularly imprinted device for the on-line analysis of AFP in human serum.

MIT is a promising strategy in antibody free analysis for tumour markers. Conventional nanosized MIPs with off-line analysis are beset by tedious operation and unsatisfactory analysis performance. In this work, an on-line analytical device to directly detect AFP, which is a typical tumour marker in cancer screening, was prepared for the first time.

Magnetic boronate modified molecularly imprinted polymers on magnetite microspheres modified with porous TiO (FeO@pTiO@MIP) with enhanced adsorption capacity for glycoproteins and with wide operational pH range.

Boronate-affinity based molecularly imprinted polymers (MIPs) are beset by the unsatisfied adsorption capacity and narrow working pH ranges. A magnetic molecularly imprinted polymer containing phenylboronic acid groups was placed on the surface of FeO (magnetite) microspheres coated with porous TiO (FeO@pTiO@MIP). In contrast to its silica analog (FeO@SiO@MIP), the flowerlike FeO@pTiO offers more binding sites for templates.

Synthesis of magnetic molecularly imprinted nanoparticles with multiple recognition sites for the simultaneous and selective capture of two glycoproteins.

Many diseases ordinarily have several kinds of biomarkers (most of them belong to the glycoprotein family). Simultaneous capturing of multiple target glycoprotein biomarkers can obtain better specificity compared with single marker detection in early screening of many diseases. In this work, we attempted to prepare magnetic molecularly imprinted nanoparticles with multiple recognition sites.

Improved surface imprinting based on a simplified mass-transfer process for the selective extraction of IgG.

We wished to improve the unsatisfactory adsorption and elution efficiencies in conventional magnetic molecular imprinting technology. In this work, a reliable strategy to prepare magnetic molecularly imprinted nanoparticles (MMINs) with rapid rebinding and elution speed was introduced. To decrease the mass-transfer retention of the templates, glycoprotein immunoglobulin (Ig)G was pre-immobilized to form homogeneous and monostratified three-dimensionally imprinted cavities on the surface of magnetic carriers.

Highly dispersed magnetic molecularly imprinted nanoparticles with well-defined thin film for the selective extraction of glycoprotein.

Antibody-free analysis is a potential method for glycoprotein analysis, but the development of this method has been limited by its unfavorable selectivity in recent years. Magnetic molecular imprinting, which integrates the fast separation of magnetic materials with high selectivity towards templates in molecular imprinting, was expected to be an effective sample pretreatment in antibody-free analysis for glycoproteins. However, the aggregation of magnetic imprinted nanoparticles and thick molecularly imprinted polymer (MIP) shells on the surface of magnetic carriers caused an unfavorable adsorption capacity, and unsatisfactory rebinding and elution rates, and has limited its application in glycoprotein extraction.

Magnetic molecularly imprinted polymer for the selective extraction of quercetagetin from Calendula officinalis extract.

A new magnetic molecularly imprinted polymers (MMIPs) for quercetagetin was prepared by surface molecular imprinting method using super paramagnetic core-shell nanoparticle as the supporter. Acrylamide as the functional monomer, ethyleneglycol dimethacrylate as the crosslinker and acetonitrile as the porogen were applied in the preparation process. Fourier transform infrared spectrometer (FT-IR), X-ray diffraction (XRD) and Vibrating sample magnetometer (VSM) were applied to characterize the MMIPs, and High performance liquid chromatography (HPLC) was utilized to analyze the target analytes.