Prebiotics-Encapsulated Probiotic Spores Regulate Gut Microbiota and Suppress Colon Cancer.

While microbial-based therapy has been considered as an effective strategy for treating diseases such as colon cancer, its safety remains the biggest challenge. Here, probiotics and prebiotics, which possess ideal biocompatibility and are extensively used as additives in food and pharmaceutical products, are combined to construct a safe microbiota-modulating material. Through the host-guest chemistry between commercial Clostridium butyricum and chemically modified prebiotic dextran, prebiotics-encapsulated probiotic spores (spores-dex) are prepared.

Artificial Super Neutrophils for Inflammation Targeting and HClO Generation against Tumors and Infections.

Neutrophils are powerful effector leukocytes that play an important role in innate immune systems for opposing tumor progression and ameliorating pathogen infections. Inspired by their distinct functions against tumors and infections, the artificial "super neutrophils" are proposed with excellent inflammation targeting and hypochlorous acid (HClO) generation characteristics for targeting and eliminating malignant tumor cells and pathogens. The "super neutrophils" are fabricated by embedding glucose oxidase (GOx) and chloroperoxidase (CPO) into zeolitic imidazolate framework-8 (ZIF-8) for HClO generation via enzymatic cascades, and then encapsulating them with the neutrophil membrane (NM) for inflammation targeting.

A two-photon excited O-evolving nanocomposite for efficient photodynamic therapy against hypoxic tumor.

This paper reported on a two-photon excited nanocomposite FCRH to overcome tumor hypoxia for enhanced photodynamic therapy (PDT). Through modified by ruthenium (Ⅱ) complex (Ru(bpy)) and hyperbranched conjugated copolymer with poly (ethylene glycol) arms (HOP), the water-splitting mediated O generation can be triggered via two-photon irradiation from iron-doped carbon nitride (Fe-CN) for the first time. While exposured to two-photon laser, Ru(bpy) was activated to generate singlet oxygen (O) and Fe-CN was triggered to split water for oxygen supply in the mean time.

A two-photon fluorescent probe for exogenous and endogenous superoxide anion imaging in vitro and in vivo.

Herein, we report a novel quinoline derivative-based two-photon fluorescent probe 6-(dimethylamino)quinoline-2-benzothiazoline (HQ), which is capable of tracking superoxide anion in organisms with specific "turn-on" fluorescence response based on extension of π-conjugations and moderate ICT process. The probe exhibited favorable photophysical properties, a broad linear range and high photostability. It can specifically detect superoxide anion with a significant fluorescence enhancement and great linearity from 0 to 500μM in PBS buffer.

A new factor influencing pathogen detection by molecular assay in children with both mild and severe hand, foot, and mouth disease.

This study aimed to find novel information concerning pathogen detection and some probable coinfection factors in hand, foot, and mouth disease (HFMD). In this study, 1104 clinically diagnosed HFMD patients were included. Enterovirus 71 (EV71), coxsackievirus A16 (CA16), and 14 different respiratory pathogens were examined from nasopharyngeal swabs using polymerase chain reaction (PCR) or reverse transcriptase PCR (RT-PCR).

Quercetin inhibit human SW480 colon cancer growth in association with inhibition of cyclin D1 and survivin expression through Wnt/beta-catenin signaling pathway.

Aim: The Wnt signaling pathway plays a pivotal role in cellular developmental processes and human carcinogenesis. The aim of this study was to investigate the effects of quercetin on the growth of the colon carcinoma cell line and the regulation effect of quercetin on the Wnt/beta-catenin signaling pathway.

Methods: MTT assay was used to determine the reduction of cell viability of quercetin on SW480 cells and clone 26 cells.

[The effect on the differentiation and maturation of dendritic cells by lupane acetate of cortex periplocae].

Aim: To investigate the effect of lupane acetate of cortex periplocae (CPLA) on the differentiation, maturation and immune activity of human peripheral blood mononuclear cell (PBMC)-derived dendritic cells (DCs) in vitro.

Methods: PBMC isolated from human peripheral blood was cultured with GM-CSF, IL-4 for 5 d and stimulated with TNF-alpha (as positive control) or CPLA to induce DCs. The morphological characteristics of DC were observed under inverted microscope and transmisson electron microscope.

[Study on the auxochrome of the electronic spectrum of organic compounds and its effect mechanism (II)].

The interaction between the p orbit of the auxochrome, the maximum bonding orbit (pi) and the minimum antibonding orbit (pi*) of the chromophore forms three new molecular orbit, among which the antibonding orbit (pi*') has the highest energy that is higher than those of the original pi*. The maximum occupied orbit (pi') will have energy lower or higher than those of pi according to different auxochrome and chromophore, and the energy of n orbit will remain steady, because the n orbit of the original chromophore is perpendicular to the p orbit of the auxochrome and the interaction is negligeable. As a result, the absorption wavelength of transition n-->pi* will shift towards higher photon energy, but for transition pi-->pi*, the absorption wavelength will shift towards either lower or higher photon energy, depending on the species of auxochrome and chromophore.

[Study on the auxochrome of the electronic spectrum of organic compounds and its effect mechanism (I)].

This paper discusses the effect mechanism of auxochrome of electronic spectrum. The study proves that the auxochrome does not invariably make the maximum absorption wavelength of chromophore shift towards lower photon energy. For the transition of n-->pi*, the p orbit of the auxochrome interacts with the minimum antibonding empty orbit(pi*), which will make the energy of pi* increase, while the p orbit of the auxochrome is perpendicular to the n orbit of chromophore, and the energy of the n orbit will remain steady ultimately, so the transition energy of n-->pi* will increase.