[Effects of BDNF antibody block on IL-6 expression in lung of rats subjected to brain ischemia].

Objective: To explore effect of brain-derived neurotrophic factor (BDNF) on IL-6 expression in the lung of rats with brain ischemia.

Methods: Inflammatory lung injury was induced by brain ischemia in rats that were devided into sham operation group, brain ischemia lung injury (BILI) group and brain ischemia with BDNF administration group. Lungs were harvested from rats in each group in 3 days after brain ischemia respectively.

[Expression of TNF-alpha in lung tissue of rats with lung injury induced by brain ischemia].

Objective: To investigate the expression of tumor necrosis factor-alpha (TNF-alpha) in the lung tissue of rats with lung injury induced by brain ischemia.

Methods: The rat model of lung injury induced by brain ischemia was established. At 24 h, 48 h, 72 h after brain ischemia, lung tissues were harvested from each rats, the expressions of TNF-alpha mRNA and protein and its distributions in the lung tissue were measured by the methods of RT-PCR (n=8), Western blot (n=8), and immunohistochemistry (n=5), respectively.