Use of Period Analysis to Timely Assess Five-Year Relative Survival for the Patients With Bone Cancer.

Background: While timely assessment of long-term survival for patients with bone cancer is essential for evaluation on early detection and prognosis level of treatment of bone cancer, those data are extremely scarce in China. We aimed to timely and accurately assess long-term survival for patients with bone cancer in Eastern China.

Methods: Patients diagnosed with bone cancer during 2004 - 2018 from four cancer registries with high-quality data from Taizhou, Eastern China were included.

Study of Grain Growth in a Ni-Based Superalloy by Experiments and Cellular Automaton Model.

The grain growth behavior in a typical Ni-based superalloy was investigated using isothermal heat treatment experiments over a holding temperature range of 1353-1473 K. The experimental results showed that the grain structure continuously coarsened as the holding time and holding temperature increased during heat treatment. A classical parabolic grain growth model was used to explore the mechanism of grain growth under experimental conditions.

[Elemental Analysis of Alloys with Picosecond Dual-Pulse LA-LIBS under Low Sample Destruction].

A study on elemental analysis of alloy samples under low sample destruction with dual-pulse laser-ablation laser induced breakdown spectroscopy (LA-LIBS) based on one picosecond Nd : YAG laser is presented. In LA-LIBS, low pulse energy 532 nm laser was used for sample ablation and high pulse energy, time–delayed 1 064 nm laser was used for re-excitation of the ablated samples to enhance atomic emissions of the laser-induced plasma and signal detection sensitivity. The influence of pulse energies of the ablation laser and excitation laser to the signal intensities was studied experimentally.

[Research Advances on Gliding-associated Proteins of Toxoplasma gondii].

The ability to invade host cells is a key to the survival and pathogenicity of Apicomplexan parasites. Toxoplasma gondii is an obligatory intracellular parasite. Its motility, invasion into, and egression from host cells are powered by a machinery called acto-myosin motor （AMM）.

[Research Advance on Toxoplasma gondii Rhoptry Protein 2 Family as Candidate Vaccine Molecules].

Toxoplasma gondii rhoptry protein 2 family (ROP2 family), secreted by the rhoptry, plays an important role in T. gondii invasion of host cells and its virulence. The ROP2 family members include ROP2, ROP4, ROP5, ROP8, ROP13, ROP16, ROP17, and ROP18.

[Quantitative Analysis of Copper Impurity in Silver Jewellery by Laser-Ablation Laser-Induced Breakdown Spectroscopy].

High spectral analysis sensitivity can be achieved with orthogonal dual-wavelength dual-pulse laser-ablation laser-induced breakdown spectroscopy under minimal sample ablation. Therefore, the contradiction between spatial resolution and analytical sensitivity existed in single-pulse laser-induced breakdown spectroscopy can be resolved fundamentally in this technique. In order to eliminate the influence of different experimental parameters to the signal intensities and final results of quantitative analysis, the correlation between copper atomic emission and silver atomic emission was studied experimentally in this technique for silver jewellery samples.

[Research progress on uridine phosphorylase in vertebrate and parasites].

Uridine phosphorylase (UPP) is a key enzyme of pyrimidine salvage pathways, catalyzing the reversible phosphorolysis of ribosides of uracil to nucleobases and ribose 1-phosphate. UPP plays an important role in the regulation of uridine homeostasis. Although UPP from a variety of organisms have many similarities in their functions, there are differences in many other aspects, such as physical and chemical properties, structure characteristics, active sites, and substrate binding sites.

Down-regulation of human enteric antimicrobial peptides by NOD2 during differentiation of the paneth cell lineage.

Ileal Crohn's disease (CD) arising from the alteration of intestinal homeostasis is characterized by two features, namely a decrease in Paneth cell-produced antimicrobial peptides that play a key role in maintaining this balance and an increase in NOD2, an intracellular sensor. Although mutations in NOD2 are highly correlated with the incidence of CD, the physiological role of NOD2 in intestinal immunity remains elusive. Here, we show that NOD2 can down-regulate the expression of human enteric antimicrobial peptides during differentiation of the Paneth cell lineage.

Protective immunity induced by peptides of AMA1, RON2 and RON4 containing T-and B-cell epitopes via an intranasal route against toxoplasmosis in mice.

Background: Toxoplasma gondii is a ubiquitous protozoan intracellular parasite, the causative agent of toxoplasmosis, and a worldwide zoonosis. Apical membrane antigen-1 (AMA1) and rhoptry neck protein (RON2, RON4) are involved in the invasion of T. gondii.

Increased expression and possible role of chitinase 3-like-1 in a colitis-associated carcinoma model.

Aim: To investigate the possible role of chitinase 3-like-1 (CHI3L1) in the progression of colitis-associated carcinoma (CAC).

Methods: Thirty-four Balb/c mice were randomly assigned to five groups, including the control, CAC control, CAC + caffeine, colitis control and colitis + caffeine. Three animals were sacrificed every two weeks for blinded macroscopic inspection, histological analysis, and total RNA extraction.

[Gene-cloning, expression and immunoreactivity detection of Toxoplasma gondii uridine phosphorylase].

Objective: To predict the physicochemical properties and antigenic epitopes of Toxoplasma gondii uridine phosphorylase (TgUPase), clone, and express TgUPase gene, and analyze its immunoreactivity.

Methods: The physical and chemical characters and specific epitopes of TgUPase protein were predicted by bioinformatics software tools. Total RNA was extracted from RH strain T.

[Cloning and expression of actin gene of Toxoplasma gondii].

Objective: To clone and express the actin gene of Toxoplasma gondii, and analyze the immunoreactivity of the recombinant protein.

Methods: Total RNA was extracted from tachyzoites of RH strain of T. gondii.

[Delivery of megawatts high energy laser pulse with large core diameter silica fiber and its application in dual-wavelength laser-ablation laser-induced breakdown spectroscopy].

To resolve the contradiction between spatial resolution and analysis sensitivity in single pulse laser-induced breakdown spectroscopy (LIBS), a study on dual-wavelength laser-ablation laser-induced breakdown spectroscopy (LA-LIBS) was carried out by using one Nd : YAG laser which was capable of two laser beam outputs with different wavelengths, where, the second harmonic output, 532 nm laser beam, was used as laser-ablation source, and the fundamental output, 1064 nm laser beam, was delivered with a large core diameter silica fiber to realize nanoseconds time-delay and then used to breakdown the ablated samples. Two laser beams were orthogonally arranged to realize element analysis with high spatial resolution and high sensitivity. Some key techniques on the coupling of 1064 nm laser beam into fiber, the collimation of laser at the fiber end and re-focusing of the laser beam were studied.

[Laser ignition assisted spark-induced breakdown spectroscopy for element analysis of aluminum alloy with enhanced sensitivity].

The analytical performance of laser ignition assisted spark-induced breakdown spectroscopy (LI-SIBS) for the analysis of trace metal in aluminum alloy was reported in the present article. In order to improve the analytical performance of spark-induced breakdown spectroscopy, a low energy laser pulse was focused on the surface of the sample to produce plasma between discharge electrodes to trigger high voltage spark discharge. Under current geometrical arrangement, optimized discharge voltage and capacitance were determined, and copper in aluminum alloy was analyzed under optimized experimental condition.

[Efficacy of albendazole orally administered at different dosages against Trichinella spiralis encapsulated larvae in mice].

Objective: To evaluate the efficacy of albendazole (ABZ) orally administered at different dosages against Trichinella spiralis encapsulated larvae in striated muscle in mice.

Methods: A total of 72 BALB/c mice were divided equally into 9 groups. Each mouse was infected orally with 50 T.

[Research progress in phosphoglycerate mutase].

Phosphoglycerate mutase (PGAM) is one of glycolytic enzymes, concerning with the transport of carbohydrates, metabolism, catalytic activity and growth development. PGAM was discovered in yeast firstly, and with its amino acid sequence and crystal structure determined, this protein was found in varies organism, such as human, Escherichia coli, Schistosoma japonicum and Toxoplasma gondii. This article reviews the physico-chemical property and research progress of PGAM of vertebrate, invertebrate and protozoa.

[Effect of oral administration of tribendimidine at different dosages against Trichinella spiralis encapsulated larvae in mice].

Objective: To observe the efficacy of oral administration of tribendimidine (TBD) at different dosages against Trichinella spiralis encapsulated larvae in murine striated muscle.

Methods: A total of 88 BALB/c mice were divided equally into 11 groups. Each mouse was infected orally with 50 T spiralis encapsulated larvae.

[Rapid measurement of trace mercury in aqueous solutions with optical-electrical dual pulse LIBS technique].

A wood slice was used as absorber to transfer liquid sample to solid sample in order to solve the problems existing in directly analyzing aqueous solutions with laser-induced breakdown spectroscopy (LIBS). An optical-electrical dual pulse LIBS (OEDP-LIBS) technique was first used to enhance atomic emission of mercury in laser-induced plasma. The calibration curves of mercury were obtained by typical single pulse LIBS and OEDP-LIBS techniques.