[Synthesis of asymmetric tetraarylporphyrins and its ytterbium complexes].

The synthesis of asymmetric meso-aryl-substituted porphyrins containing three 4-methoxycarbonylphenyl groups, and as a forth substituent 4-hydroxyphenyl or 4-hydroxy-3- methoxyphenyl radicals, or the isomeric 3- and 4-pyridyl substituents is described. O-alkyl derivatives of 4-hydroxyl residue are obtained. The ytterbium complexes ofthese porphyrins were synthesized and studied their luminescence spectral properties were studied.

[Synthesis of tetrasubstituted porphyrins by interaction of 5-aryldipyrromethanes with 4-substituted benzaldehydes].

Synthesis of tetraphenyl-substituted porphyrins with tret-butyl and methoxycarbonyl groups in meso-aryl radicals is described. It is shown that, during the condensation of dipyrromethanes with substituted benzaldehydes, a rearrangement occurs with the formation of a mixture of isomeric porphyrins. The character of these rearrangements depends on the position of substituents in the starting compounds.

[Synthesis and luminescent spectral characteristics of porphyrin complexes with platinum group metals].

The synthesis of natural and synthetic porphyrin complexes with Pt, Pd, Rh, and Ru is reported. Their electronic absorption spectra, phosphorescence spectra, and lifetimes at room temperature both in the presence and in the absence of oxygen were studied. It has been shown that the variation of the nature of the central metal atom and of the substituents in pyrrole and phenyl rings allows the obtaining of metalloporphyrins with various phosphorescence excitation and phosphorescing emission spectra at room temperature.

[Boradiazaindacene dyes for technology of biological microchips].

A number of boradiazaindacene dyes containing a carboxyl group separated from a fluorophore by two methylene units were synthesized. The compounds have narrow spectral bands with absorption maxima at 480-530 nm and fluorescence maxima at 500-550 nm. Succinimide esters of these compounds and the corresponding fluorescent-labeled olgionucleotides were also prepared.

[Formylation of metallocomplexes of tetra-meso-(para- and meta-methoxyphenyl)porphyrines].

The Vilsmeier formylation of metallocomplexes of isomeric meta- and para-methoxy-substituted tetraphenylporphyrines has was been studied. Formyl derivatives of meta-isomers were shown to form cyclization products not only in solutions, but also in the the solid state. The ability to undergo such transformations decreases in the trend following order: Co > Cu > Ni > Pd > Pt.

[Formylation of porphyrin platinum complexes].

The formylation reaction of platinum complexes of beta-unsubstituted porphyrins was studied. The interaction of deuteroporphyrin IX derivatives with the Vilsmeyer reagent led to the selective formylation of their macrocycles in the beta position. The resulting formyl derivatives of the porphyrins are of interest for fluorescent immunoassay.

[Synthesis of conjugates of bovine serum albumin with water-soluble ytterbium porphyrins].

The ytterbium complex of 5,10,15,20-tetrakis(4-carboxyphenyl)porphyrin was synthesized as an IR-fluorescent label and covalently bound to bovine serum albumin. The resulting conjugate fluoresces at 985 nm and is of interest for use in IR-fluorescent tumor diagnostic, immunoassay, and energy transfer studies. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol.

[Optical and photoelectrochemical properties of Langmuir films of a Zn-complex of ethioporphyrin II].

The optical and photoelectrochemical properties of Zn ethioporphyrin II (ZnEP) Langmuir films deposited on an SnO2-optically transparent electrode were investigated. Absorption and fluorescence spectra were utilized to control the film structure. The main three factors, in our opinion, act on the photovoltage, namely, film thickness, ZnEP aggregate formation and the total ZnEP concentration in the monolayer.

[Fluorescence of meso-tetrakis(4-(carboxy)phenyl)porphine covalently bound to oligonucleotides d(CG)5 and d(TA)5].

The amino-reactive derivative of tetraphenylporphine meso-tetrakis[4-(carboxy)phenyl]porphine (TCPP) was synthesized, which is characterized by a high molar absorption coefficient (epsilon 416 = 36,500 M-1.cm-1). TCPP was covalently attached to oligonucleotides d(CG)5 [d(CG)5-TCPP] and d(TA)5 [d(TA)5-TCPP].

[Biosynthesis and use of coproporphyrins and uroporphyrins and their metal complexes in immune analysis and diagnostic methods].

Methods of synthesis of coproporphyrin and uroporphyrin by using bacteria of the genus Arthrobacter are proposed. Metal complexes of coproporphyrin and uroporphyrin with Pt, Pd, and Zn were synthesized. Their structures were identified by spectrophotometry, IR spectrometry, 1H-NMR, mass spectrometry, and HPLC.

[Halogenation in a series of metallocomplexes of porphyrin].

We found that thionyl chloride can chlorinate porphyrin complexes with transient metals (Pd, Ni, or Cu) at the free beta- and meso-positions of the porphyrin macrocycle. A more prolonged or rigorous treatment also causes the chlorination of side alkyl substituents, mainly, methyl groups.

[Characteristics of Triticum vulgare in the early phases of development (ontogenesis) in space flight].

The effect of space flight factors on the growth and development of wheat grown in a Svetoblock-M unit on the orbital station Mir was investigated. The data obtained allow the conclusion that wheat plants develop flight-induced changes. When compared to control plants, experimental plants show a delayed growth in terms of biometric parameters, different distribution of biogenic elements, lower phytoncidal activity, increased bacterial and fungal contamination, and a changed pigment and lipid composition.

[Preparation and characteristics of protoplasts of Streptomyces kanamyceticus].

To prepare actively regenerating protoplasts of S. kanamyceticus, the influence of the conditions of the mycelium cultivation, the culture age, lytic conditions, composition of the regeneration medium, the procedure of the culture inoculation to the regeneration medium and other parameters were studied. The study resulted in development of optimal conditions for preparation of S.

[Development of protoplasts of Streptomycetes on media favoring the regeneration and formation of L-forms].

The protoplasts of three Streptomyces species and their regenerative ability were studied using light microscopy. When Streptomyces lividans and S. erythraeus protoplasts are cultivated on regeneration media, their regeneration is not synchronous during the first day; some protoplasts revert to yield the mycelial form and also L-forms of these cultures are produced.

[Preparation and transplantation of demineralized bone tissue].

The use of demineralized bone transplants is found to increase the effectiveness of allogenic transplantations of bone tissue due to its ability to induce active osteogenesis even when implanted intramuscularly. The method of preparing the bone transplants by demineralization in solutions of hydrochloric acid should be widely used in traumatology and orthopedy.

[Pathologicoanatomic data on metastasis of the stomach cancer to the ovaries].

The results of evaluation of the data on autopsies for stomach cancer provided by the pathology departments of the hospitals of Leningrad (1975-1979) and Petrozavodsk (1965-1979) are presented. Metastases into ovaries were found in 98 cases. i.

[Chromatographic and spectral characteristics of liposoluble fluorescent compounds accumulated during damaging and aging of plants].

The chromatographic properties (e. g. thin layer mobility, separation by molecular adsorption and gel chromatography) and the fluorescence parameters of liposoluble fluorescent compounds (LFC) accumulated in the tissues during aging of detached leaves, in autumn leaves of deciduous plants and in the leaves treated with diquat, 2,4-dichloro- and 2,4,5-trichlorophenoxyacetic acids were studied.

[Symptom dynamics in the surgical treatment of posttraumatic arthrosis deformans of the knee joint].

288 cases of posttraumatic deforming arthrosis of the knee-joint were examined. The further postoperative periodic examinations and checking of the late results of the treatment of 171 patients have proved that the symptoms of the disease are mainly due to the disorders in the knee-joint stability. The obtained data suggest the possibility of a secondary inflammatory origin of pain in deforming arthrosis and witness an important role played by the surgical prevention and correction of the joint instability in clinical remission of th disease.

[Comparative study of the effect of supercooling and freezing on fungal cells].

Observations of the reactivation rate and subsequent growth in addition to the luminescence of cells subjected to supercooling and freezing at 15 degrees C suggested that the effect of supercooling is more significant than that of freezing. In supercooled suspensions, cells are more injured than in frozen ones and the number of injured cells increases more rapidly. Unlike the supercooled cells, the live cells from frozen suspensions maintain a higher growth activity for a longer time.