Estimated lower speed boundary at which the walk ratio constancy is broken in healthy adults.

[Purpose] The ratio of step length to cadence (walk ratio) is invariant over a wide range of speeds. However, no studies have investigated details of the change in the walk ratio at slow speeds. It is necessary to explore how walking behavior changes at a low speed to understand the slow walking observed in various conditions such as aging and pathological conditions.

Transplantated mesenchymal stem cells derived from embryonic stem cells promote muscle regeneration and accelerate functional recovery of injured skeletal muscle.

We previously established that mesenchymal stem cells originating from mouse embryonic stem (ES) cells (E-MSCs) showed markedly higher potential for differentiation into skeletal muscles in vitro than common mesenchymal stem cells (MSCs). Further, the E-MSCs exhibited a low risk for teratoma formation. Here we evaluate the potential of E-MSCs for differentiation into skeletal muscles in vivo and reveal the regeneration and functional recovery of injured muscle by transplantation.

Mesenchymal stem cells originating from ES cells show high telomerase activity and therapeutic benefits.

We establish a novel method for the induction and collection of mesenchymal stem cells using a typical cell surface marker, CD105, through adipogenesis from mouse ES cells. ES cells were cultured in a medium for adipogenesis. Mesenchymal stem cells from mouse ES cells were easily identified by the expression of CD105, and were isolated and differentiated into multiple mesenchymal cell types.