A new pyrrolopyrazine alkaloid from the fruit of .

A new pyrrolopyrazine alkaloid, named lycipyrazine A (), alone with seven known alkaloids (-), were isolated from the fruit of The structure of new compound was determined using extensive 1D and 2D spectroscopic analyses, while its absolute configuration was determined by comparison of its experimental and calculated ECD spectra. Their -glucosidase inhibitory activity was screened using acarbose as a positive control. Compound showed strong inhibitory activity against -glucosidase (IC = 3.

Effects of D-Amino Acid Replacements on the Conformational Stability of Miniproteins.

For many proteins, proper function requires adoption of a specific tertiary structure. This study explores the effects of L-to-D amino acid substitutions on tertiary structure stability for two well-known miniproteins, a single-site variant of the chicken villin headpiece subdomain (VHP) and the human Pin1 WW domain (WW). For VHP, which features an α-helix-rich tertiary structure, substitutions led to significant destabilization, as detected by variable temperature circular dichroism (CD) measurements.

Geometrical Features of Subbasal Corneal Whorl-like Nerve Patterns in Dry Eye Disease: An In Vivo Confocal Microscopy Study.

Purpose: To investigate the geometrical feature of the whorl-like corneal nerve in dry eye disease (DED) across different severity levels and subtypes and preliminarily explore its diagnostic ability.

Design: Cross-sectional study.

Participants: The study included 29 healthy subjects (51 eyes) and 62 DED patients (95 eyes).

Homologous Peptide Foldamer Promotes FUS Aggregation and Triggers Cancer Cell Death.

Fused in sarcoma (FUS), a multifunctional deoxyribonucleic acid (DNA)/ribonucleic acid (RNA)-binding protein, has been implicated in various cancer types, including sarcoma and leukemia. Despite its association with these diseases, there has been limited exploration of FUS as a cancer therapy target, primarily because its dynamic nature makes it difficult to target specifically. In this study, we explored a kind of β-sheet peptide foldamer, named β-TAT, to influence FUS aggregation by targeting its RNA recognition motifs (RRM).

Harnessing Aromatic-Histidine Interactions through Synergistic Backbone Extension and Side Chain Modification.

Peptide engineering efforts have delivered drugs for diverse human diseases. Side chain alteration is among the most common approaches to designing new peptides for specific applications. The peptide backbone can be modified as well, but this strategy has received relatively little attention.

The Fried Frailty Phenotype in Patients Undergoing Cardiac Surgery: A Systematic Review and Meta-Analysis.

Objective: Frailty is an increasingly recognized marker of poor surgical outcomes in cardiac surgery. Frailty first was described in the seminal "Fried" paper, which constitutes the longest-standing and most well-recognized definition. This study aimed to assess the impact of the Fried and modified Fried frailty classifications on patient outcomes following cardiac surgery.

Metastatic giant cell tumour of bone: a narrative review of management options and approaches.

Giant cell tumour of bone (GCTB) is a locally aggressive bone neoplasm with a rare tendency to metastasise, most commonly to the lungs. The management of metastatic GCTB (metGCTB) is controversial due to its unpredictable behaviour. Asymptomatic patients should be monitored radiologically and undergo treatment only when disease progression occurs.

First genetic assessment of brackish water polychaete : mitochondrial sequences reveal strong genetic differentiation and population expansion in samples collected from southeast China and north Vietnam.

is a widespread benthic polychaete worm found in coastal brackish waters of the west Pacific. It has high ecological and economic value as a biomarker of water quality and as a high-quality feed in aquaculture and fisheries and is considered a delicacy in some areas of Asia. However, it has experienced a marked reduction in recent years due to overexploitation as well as changes in the environment and climate.