Blimp1 suppressed CD4 T cells-induced activation of fibroblast-like synoviocytes by upregulating IL-10 via the rho pathway.

Background: B lymphocyte-induced maturation protein 1 (Blimp1) is a risk allele for rheumatoid arthritis (RA), but its functional mechanism in RA remains to be further explored.

Methods: Flow cytometry was performed to detect CD4 T cell differentiation. ELISA was used to measure inflammatory factor secretion.

Total glucosides of paeony protects THP-1 macrophages against monosodium urate-induced inflammation via MALAT1/miR-876-5p/NLRP3 signaling cascade in gouty arthritis.

Background: Monosodium urate (MSU)-mediated inflammatory response is a crucial inducing factor in gouty arthritis. Here, we explored the underlying mechanism of total glucosides of paeony (TGP) in MSU-induced inflammation of THP-1 macrophages in gouty arthritis.

Methods: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to detect cell viability.

Determination of creatinine, uric and ascorbic acid in bovine milk and orange juice by hydrophilic interaction HPLC.

Creatinine (Cr), uric (UA) and ascorbic acid (AA) are common constituents in human fluids. Their abnormal concentrations in human fluids are associated with various diseases. Thus, apart from the endogenous formation in human body, it is also important to examine their sources from food products.

Association of coffee drinking with all-cause mortality: a systematic review and meta-analysis.

Objective: We aimed to use the meta-analysis method to assess the relationship between coffee drinking and all-cause mortality.

Design: Categorical and dose-response meta-analyses were conducted using random-effects models.

Setting: We systematically searched and identified eligible literature in the PubMed and Scopus databases.