A novel detection of hydrogen peroxide based on a luminescent polyoxometalate.

In this paper, we attempt to construct a simple and sensitive detection method for hydrogen peroxide based on reversible colour change and luminescence switching modulated by the electrochemical stimulation and redox reaction of H(2)O(2) with electroreduced polyoxometalate. This method successfully combines the electrochromic and luminescent properties of the polyoxometalate to develop a novel detection method for H(2)O(2) with good reversibility, which displays high sensitivity, a wide linear range and a low detection limit to H(2)O(2).

The evaluation of recombinant, chimeric, tetravalent antihuman CD22 antibodies.

Purpose: The purpose of this study was to prepare chimeric antihuman CD22 tetravalent monoclonal antibodies (MAbs) with high functional affinity, long persistence in the circulation, increased antitumor activity, and conserved effector function in vitro.

Experimental Design: We investigated the association/dissociation rates of these tetravalent antibodies using CD22(+) Daudi lymphoma cells. We then tested their ability to interact with Fc receptors on a human cell line (U937), to mediate antibody-dependent cellular cytotoxicity with human natural killer cells, to bind human C1q, to inhibit the in vitro growth of CD22 Daudi cells, and to persist in the circulation.