PerR functions as a redox-sensing transcription factor regulating metal homeostasis in the thermoacidophilic archaeon Saccharolobus islandicus REY15A.

Thermoacidophilic archaea thrive in environments with high temperatures and low pH where cells are prone to severe oxidative stress due to elevated levels of reactive oxygen species (ROS). While the oxidative stress responses have been extensively studied in bacteria and eukaryotes, the mechanisms in archaea remain largely unexplored. Here, using a multidisciplinary approach, we reveal that SisPerR, the homolog of bacterial PerR in Saccharolobus islandicus REY15A, is responsible for ROS response of transcriptional regulation.

Chiral Cobalt Nanocluster-Driven Chemiluminescent System for the Facile Discrimination of Carnitine Enantiomers with a Hydrogel-Assisted Strategy.

Facile chiral discrimination without relying on chromatographic techniques has long been a huge challenge due to the minimal differences in the physicochemical properties of enantiomers. Polysaccharide hydrogels with natural chiral selectivity can be promising materials for constructing chiral discrimination platforms. In this study, l-cysteine-induced cobalt nanoclusters (LC-CoNCs) were prepared and utilized as chiral nanozymes for promoting the chemiluminescent (CL) signal of a luminol-HO system.

Unlocking multi-mode sensing potential: Phosphorus-doped graphitic carbon nitride quantum dots for Ag, ciprofloxacin, and riboflavin analysis in environment and food matrices.

The simultaneous detection of multiple analytes through a single fluorescence sensor is highly attractive. In this study, phosphorus-doped graphitic carbon nitride quantum dots (P-CNQDs) were developed, achieving multi-mode sensing through three distinct response mechanisms. The preparation involved using melamine as the carbon and nitrogen source and ammonium dihydrogen phosphate as the phosphorus source.

Layer Growth Inhibiting Strategy for Superior-Loading Atomic Metal Sites on Ultrathin Layered Double Hydroxides as the Efficient Chemiluminescence Probes.

Owing to the remarkable catalytic attributes, single-atom catalysts (SACs) have exhibited promising application prospects as the substitutes of natural enzymes. However, the low loading amount of atomic sites on typical SACs (no more than 5 wt %) significantly restricts their increased capability. Hereby, a layer growth inhibitor protocol was attempted to optimize anchoring isolated Co atoms efficiently on ultrathin monolayer layered double hydroxides (LDHs).

Exploring the diversity of microbes and natural products from fungus-growing termite tripartite symbiosis.

The fungus-growing termite is considered a distinct ecological niche because it involves a tripartite symbiosis between the termite host, gut microflora, and the fungus , which has led to the expansion of highly organized and complex societies among termite colonies. Tripartite symbiosis in fungus-growing termites may promote unique microbes with distinctive metabolic pathways that may serve as valuable resources for developing novel antimicrobial therapeutic options. Recent research on complex tripartite symbioses has revealed a plethora of previously unknown natural products that may have ecological roles in signaling, communication, or defense responses.

Facile synthesis of carbon nitride quantum dots as a highly selective and sensitive fluorescent sensor for the tetracycline detection.

Enhanced blue fluorescent carbon nitride quantum dots (g-CNQDs) were synthesized by a simple solvothermal "tailoring" process from bulk g-CN and analyzed by various characterization methods. The as-obtained g-CNQDs were successfully applied in the determination of tetracycline (TC) with a good linear relationship in the range of 0.23-202.

Development and comparison of UPLC-ESI-MS and RP-HPLC-VWD methods for determining microcystin-LR.

Microcystin-LR (MC-LR) generated by cyanobacteria is a kind of potent hepatotoxin, which poses a considerable threat to human health. In the research field of MC-LR removal, the quantitative analysis in a wide concentration range of samples is inevitable. In this paper, we presented the pseudo united use of an Ultra Performance Liquid Chromatography Mass Spectrometry (UPLC-MS) and High Performance Liquid Chromatography system with a Variable Wavelength Ultraviolet Detector (HPLC-VWD) approach to detect MC-LR.