Bright and Stable Cyan Fluorescent RNA Enables Multicolor RNA Imaging in Live Escherichia coli.

Fluorescent RNAs (FRs), which are RNA aptamers that bind and activate their cognate small fluorogenic dyes, have provided a particularly useful approach for imaging RNAs in live cells. Although the color palette of FRs is greatly expanded, a bright and stable cyan FR with good biocompatibility and biorthogonality with currently available FRs remains desirable but is not yet developed. Herein, the development of Myosotis is described, an RNA aptamer that emits bright cyan fluorescence upon binding a novel GFP chromophore-like fluorophore called DBT.

Rapid detection of microplastics/nanoplastics directly exposed to blood during intravenous injections via mie scattering spectra.

Microplastics/nanoplastics (M/NPs) are pervasive in the environment, leading to inevitable human exposure through various pathways and raising significant public and scientific concern. Understanding the sources and levels of M/NPs in human blood is crucial for environmental health studies. This work examined the content, type, shape, and size of M/NPs released directly into the bloodstream from medical devices via saline solution during intravenous (IV) injection.

Genome-wide identification of the grapevine β-1,3-glucanase gene (VviBG) family and expression analysis under different stresses.

Background: The β-1,3-glucanase gene is widely involved in plant development and stress defense. However, an identification and expression analysis of the grape β-1,3-glucanase gene (VviBG) family had not been conducted prior to this study.

Results: Here, 42 VviBGs were identified in grapevine, all of which contain a GH-17 domain and a variable C-terminal domain.

Distinctive patterns of bacterial community succession in the riverine micro-plastisphere in view of biofilm development and ecological niches.

Exploring plastic bacterial community succession is a crucial step in analyzing and predicting the ecological assembly processes of the plastisphere and its associated environmental impacts. However, microbial biofilm development and niche differentiation during plastic bacterial community succession have rarely scarcely considered. Here, we assessed the differences between three microplastics (MPs) and two natural polymers in terms of biofilm development and niche properties during bacterial community succession, and identified a genus of MPs-degrading bacteria with strong competitive potential in the plastisphere.

High-Throughput Single-Cell Metabolic Labeling, Sorting, and Sequencing of Active Antibiotic-Resistant Bacteria in the Environment.

Active antibiotic-resistant bacteria (ARB) play a major role in spreading antimicrobial resistance (AMR) in the environment; however, they have remained largely unexplored. Herein, we coupled bio-orthogonal noncanonical amino acid tagging with high-throughput fluorescence-activated single-cell sorting (FACS) and sequencing to characterize the phenome and genome of active ARB in complex environmental matrices. Active ARB, conferring resistance to six antibiotics throughout wastewater treatment, were distinguished and quantified.

Rhizospheric bacterial communities against microplastics (MPs): Novel ecological strategies based on the niche differentiation.

Considerable amounts of microplastics (MPs) are stocked in plant rhizospheres, yielding adverse effects on rhizospheric microorganisms and threatening plant health. However, the adaptation of the rhizospheric microbiota for MPs remains largely unknown. Here, to evaluate the adaptive strategies of rhizospheric bacterial communities against MPs, we characterized the spatial dissimilarities in MPs properties and bacterial communities from mangrove non-rhizosphere to rhizosphere to root hair sediments.

Supported Au single atoms and nanoparticles on MoS for highly selective CO-to-CHCOOH photoreduction.

Effectively controlling the selective conversion of CO photoreduction to C products presents a significant challenge. Here, we develop a heterojunction photocatalyst by controllably implanting Au nanoparticles and single atoms into unsaturated Mo atoms of edge-rich MoS, denoted as Au/Au-CMS. Photoreduction of CO results in the production of CHCOOH with a selectivity of 86.

Fate and drivers of mariculture-derived microplastics from ponds to mangrove forests.

Due to the combined influences of marine and terrestrial disturbances, the sources of microplastics (MPs) in mangrove ecosystems are complex and diverse. Previous studies have inferred the possible involvement of mariculture activities as a potential source of mangrove MPs based on the characteristics of MPs. However, the direct contributions of mariculture-derived MPs to mangrove MPs remain largely unknown.

Source and succession of microbial communities and tetramethylpyrazine during the brewing process of compound-flavor Baijiu.

Pyrazines are important flavor components and healthy active components in Baijiu, which including tetramethylpyrazine (TTMP). During the brewing process, the traceability of microbial communities and the content distribution characteristics of TTMP are important for improving the quality and style characteristics of compound-flavored Baijiu (CFB). However, the traceability analysis of microorganisms in fermented grains (FG)-used in the production of CFB-lacks quantitative and systematic evaluation.

Travertine deposition rather than tourism activity is the primary contributor to the microplastic risks in alpine karst lakes.

Microplastics (MPs) are emerging as anthropogenic vectors to form plastisphere, facilitating microbiome colonization and pathogenic dissemination, thus contributing to environmental and health crises across various ecosystems. However, a knowledge gap persists regarding MPs risks and their driving factors in certain unique and vulnerable ecosystems, such as Karst travertine lakes, some of which are renowned World Natural Heritage Sites under ever-increasing tourism pressure. We hypothesized that tourism activities serve as the most important factor of MPs pollution, whereas intrinsic features, including travertine deposition can exacerbate potential environmental risks.

Nitrifying niche in estuaries is expanded by the plastisphere.

The estuarine plastisphere, a novel ecological habitat in the Anthropocene, has garnered global concerns. Recent geochemical evidence has pointed out its potential role in influencing nitrogen biogeochemistry. However, the biogeochemical significance of the plastisphere and its mechanisms regulating nitrogen cycling remain elusive.

PFKL promotes cell viability and glycolysis and inhibits cisplatin chemosensitivity of laryngeal squamous cell carcinoma.

Laryngeal squamous cell carcinoma (LSCC) with a high incidence and mortality rate, has a serious impact worldwide. Phosphofructokinase-1 liver type (PFKL) is a major enzyme in glycolysis progress, but its role in modulating tumorigenesis and cisplatin (DDP) chemosensitivity of LSCC was still unclear. The mRNA and protein levels of PFKL were detected by qRT-PCR and immunohistochemical assay.

Stabilizing Few-Atom Platinum Clusters by Zinc Single-Atom-Glue for Efficient Anti-Markovnikov Alkene Hydrosilylation.

Few-atom metal clusters (FAMCs) exhibit superior performance in catalyzing complex molecular transformations due to their special spatial environments and electronic states, compared to single-atom catalysts (SACs). However, achieving the efficient and accurate synthesis of FAMCs while avoiding the formation of other species, such as nanoparticles and SACs, still remains challenges. Herein, we report a two-step strategy for synthesis of few-atom platinum (Pt) clusters by predeposition of zinc single-atom-glue (Zn) on MgO nanosheets (Pt-Zn/MgO), where FAMCs can be obtained over a wide range of Pt contents (0.

A powerful method for In Situ and rapid detection of trace nanoplastics in water-Mie scattering.

The pervasive presence of nanoplastics (NPs) in environmental media has raised significant concerns regarding their implications for environmental safety and human health. However, owing to their tiny size and low level in the environment, there is still a lack of effective methods for measuring the amount of NPs. Leveraging the principles of Mie scattering, a novel approach for rapid in situ quantitative detection of small NPs in low concentrations in water has been developed.

Secreted salt and hydrodynamic factors combine to affect dynamic fluctuations of microplastics on mangrove leaves.

Mangrove leaves have been acknowledged as crucial sink for coastal microplastics (MPs). Whereas, the temporal dynamics of MPs intercepted by mangrove leaves have remained poorly understood. Here, we detected MPs intercepted by submerged and non-submerged mangrove leaves over time and the potential driving factors.

New materials-based on gelatin coordinated with zirconium or aluminum for ecological retanning.

Ecological retanning agent is an effective way to solve the pollution source of leather manufacturing industry. In this study, the gelatin from chrome-containing leather shavings in the leather industry was used to realize sustainable leather post-tanning. The gelatin hydrolysate (GH) coordinated with Zr or Al to prepare eco-friendly retanning agents GH-Zr and GH-Al.

Comparison of the Correlations of Microbial Community and Volatile Compounds between Pit-Mud and Fermented Grains of Compound-Flavor Baijiu.

The microbial composition and volatile components of fermented grains (FG) and pit mud (PM) are crucial for the quality and flavor of compound-flavor baijiu (CFB). The physicochemical indices, culturable microorganisms, microbial communities, and volatile components of FG and PM were analyzed and correlated in our research. Considering FG and PM, amplicon sequencing was used to analyze the microbial community and the volatile components were detected by headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME).

Insight into the role of niche concept in deciphering the ecological drivers of MPs-associated bacterial communities in mangrove forest.

Myriad inherent and variable environmental features are controlling the assembly and succession of bacterial communities colonizing on mangrove microplastics (MPs). However, the mechanisms governing mangrove MPs-associated bacterial responses to environmental changes still remain unknown. Here, we assessed the dissimilarities of MPs-associated bacterial composition, diversity and functionality as well as quantified the niche variations of each taxon on plastispheres along river-mangrove-ocean and mangrove landward-to-seaward gradients in the Beibu Gulf, China, respectively.

Insights into the influence of physicochemical parameters on the microbial community and volatile compounds during the ultra-long fermentation of compound-flavor Baijiu.

Introduction: While the variation in physicochemical parameters, microbial communities, metabolism, composition, and the proportion of volatile components in fermented grains (FG) affect final Baijiu quality, their complex interactions during the ultra-long fermentation of compound-flavor Baijiu (CFB) are still poorly understood.

Methods: In this study, amplicon sequencing was used to analyze the microbial community, and headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to analyze the volatile components in FG during ultra-long fermentation of CFB. The relationships between the dominant microbial communities, physicochemical parameters, and volatile components were analyzed using redundancy analysis and network analysis.

Dynamic fluctuations in plant leaf interception of airborne microplastics.

Plant leaves have been demonstrated to be a crucial sink of airborne microplastics (MPs). However, because of the particular shape of MPs and their relatively weak forces with leaves, the traditional accumulation model used for the adsorption of particulate matter and persistent organic pollutants may not be appropriate for describing the interception of MPs by leaves. Here, we performed a 7-day exploration of the interception of MPs by leaves in downtown Nanning.

Quantifying health risks of plastisphere antibiotic resistome and deciphering driving mechanisms in an urbanizing watershed.

Microplastics (MPs) ubiquitous in environments promote the dissemination of antibiotic resistance genes (ARGs), threatening ecosystem safety and human health. However, quantitative assessments of the health risks of ARGs (HRA) in plastisphere and an in-depth exploration of their driving mechanisms are still lacking. Here, the microbiomes, ARGs, and community assembly processes of five types of MPs in an urbanizing watershed were systematically investigated.