Preparation of an Immunoaffinity Column Based on Bispecific Monoclonal Antibody for Aflatoxin B and Ochratoxin A Detection Combined with ic-ELISA.

A novel and efficient immunoaffinity column (IAC) based on bispecific monoclonal antibody (BsMAb) recognizing aflatoxin B (AFB) and ochratoxin A (OTA) was prepared and applied in simultaneous extraction of AFB and OTA from food samples and detection of AFB/OTA combined with ic-ELISA (indirect competitive ELISA). Two deficient cell lines, hypoxanthine guanine phosphoribosyl-transferase (HGPRT) deficient anti-AFB hybridoma cell line and thymidine kinase (TK) deficient anti-OTA hybridoma cell line, were fused to generate a hybrid-hybridoma producing BsMAb against AFB and OTA. The subtype of the BsMAb was IgG via mouse antibody isotyping kit test.