Arsenic-induced downregulation of BRWD3 suppresses proliferation and induces apoptosis in lung adenocarcinoma cells through the p53 and p65 pathways.

Bromodomain and WD-repeat domain-containing protein 3 (BRWD3) exhibits high expression in lung adenocarcinoma (LUAD) tissues and cells; however, its function in arsenic-induced toxicological responses remains unclear. This study aimed to investigate BRWD3 expression in response to arsenic-induced conditions and its impact on the proliferation and apoptosis of LUAD cell line SPC-A1 upon BRWD3 knockdown. The results revealed a decrease in BRWD3 expression in SPC-A1 cells treated with sodium arsenite (NaAsO), but not sodium arsenite's metabolites.

An efficient cerium dioxide incorporated nickel cobalt phosphide complex as electrocatalyst for All-pH hydrogen evolution reaction and overall water splitting.

Transition metal phosphides (TMPs) have been considered as potential electrocatalysts with adjustable valence states, metal characteristics, and phase diversity. However, it is necessary but remains a major challenge to obtain efficient and durable TMPs catalysts, which can realize efficiently for not only all-pH hydrogen evolution reaction (HER), but also oxygen evolution reaction (OER). Hence, cerium dioxide incorporated nickel cobalt phosphide growth on nickel foam (CeO/NiCoP) is fabricated by hydrothermal and phosphating reaction.

Atomically dispersed Au anchored on CeOto enhancing the antioxidant activity.

The modification of Au nanoparticles can improve the antioxidant activity of CeO, however, nano Au/CeOhas also met some problems such as low atomic utilization, the limit of reaction conditions, and high cost. Au single atom catalysts can well solve the above-mentioned problems, but there are some contradictory results about the activity of single atom Au/CeOand nano Au/CeO. Here, we synthesized rod-like Au single atom Au/CeO(0.

The transcript NR 134251.1 of lncRNA APTR with an opposite function to all transcripts inhibits proliferation and induces apoptosis by regulating proliferation and apoptosis-related genes.

Arsenic (As) exposure has been a global public health concern for hundreds of millions worldwide. LncRNA APTR (Alu-mediated p21 transcriptional regulator) plays an essential role in tumor growth and development. However, its function in arsenic-induced toxicological responses is still unknown.

A simple method for the preparation of CeOwith high antioxidant activity and wide application range.

Cerium oxide (CeO) is a well-known antioxidant with the ability to scavenge reactive oxygen species due to its unique electronic structure and chemical properties. Although many methods to enhance the antioxidant activity of CeOhave been reported, its antioxidant activity is still not high enough, and some enhancement effects are limited by the material concentration. There are also some CeOobtained with high antioxidant activity at high concentrations, which is not conducive to the application of biomedicine.

Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65.

The regulatory network between arsenic, genes and signaling pathways has been reported in arsenic carcinogenesis. Studies on circRNA represent a growing field, but the extent to circRNA potential mechanisms remains poorly understood. So this study we explore the systematic function of hsa_circ_0005050 in mediating the cell apoptosis and proliferation.

CircP50 functions through the phosphorylation- and acetylation-activated p53 pathway to mediate inorganic arsenic-induced apoptosis in A549 cells.

As a class I carcinogen, arsenic has been reported to cause diseases accompanied by circRNAs regulating proliferation and apoptosis at the molecular level, but whether circP50 (circBase ID: hsa_circ_0008012) does the same has not been demonstrated. The aim of this study is to provide the basis for anti-lung cancer mechanism research, by studying the expression of circP50 under arsenic-induced conditions, and the effect and mechanism on the proliferation and apoptosis of A549 cells based on the circP50 knockdown models. To explore whether the circP50 is responsive to arsenic exposure, the qRT-PCR was applied to discover that the relative expression of circP50 in A549 cells increased only with increasing NaAsO dose and independent of its metabolites.

A lable-free SPR biosensor based on one peptide sequence with three recognition sites for O-GlcNAc transferase detection.

Abnormal O-linked N-acetylglucosamine (O-GlcNAc) concentrations have been associated with many diseases, but the lack of accurate detection method limited O-GlcNAc to be used as a biomarker in clinical diagnosis. Then O-GlcNAc transferase (OGT) has drawn researchers' attention as it closed related to the level of O-GlcNAc and be considered to be a promising new target for diseases diagnosis. Nevertheless, the existing OGT detection methods are either need labeling or the sensitity can not meet the needs of clinic testing.

Enhancement of antioxidant activity of well-dispersed core-shell structured CeO coating Au nanorods under visible light irradiation.

Gold nanoparticle (AuNP) modification shows great advantages in improving the antioxidant activity of nanoCeO. However, the improved effect of AuNP modification becomes smaller and even results in the decrease of antioxidant ability due to severe aggregation with increasing nanomaterial concentration. Additionally, the effects of photo-properties of AuNPs on the antioxidant activity of nanoCeO have not been studied.

Surface plasmon resonance biosensor for the accurate and sensitive quantification of O-GlcNAc based on cleavage by β-D-N-acetylglucosaminidase.

Abnormal O-linked-N-acetylglucosamine (O-GlcNAc) concentrations have been associated with a variety of diseases (e.g., cancer, Alzheimer's disease, cardiovascular disease, etc.

The effect of DNA on the oxidase activity of nanoceria with different morphologies.

Many nanomaterials have been reported to have enzyme-like activities and are considered as nanozymes. As a multifunctional nanozyme, nanoceria has received much attention due to the dual oxidation states of Ce/Ce which facilitate redox reactions at the particle surface. Despite the advantages of nanozymes, their limited activity and lack of enzyme specificity are still problems to be resolved.

[Application of adjustable moving overlapping separation range map in multisegment stepwise gradient elution condition optimization of high performance liquid chromatography].

Computer-assisted optimization of high performance liquid chromatographic separation conditions can be used to obtain optimized experimental conditions in relatively short time and low cost, and has been widely applied to the separation of complex samples. Based on "moving overlapping separation range map" method, a novel method of optimizing multi-segment stepwise gradient elution conditions, "adjustable moving overlapping separation range map" method, was developed. By predicting the retention time, peak width and resolution of each solute under different mobile phase conditions, the overlapping separation range map of the solutes in the sample is plotted.

Separation and identification of compounds in Rhizoma chuanxiong by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry.

A comprehensive two-dimensional liquid chromatographic separation system based on the combination of a CN column and an ODS column is developed for the separation of components in a traditional Chinese medicine (TCM) Rhizoma chuanxiong. Two columns are coupled by a two-position, eight-port valve equipped with two storage loops and controlled by a computer. The effluent is detected by both the diode array detector and atmospheric pressure chemical ionization (APCI) mass spectrometer.

[Application of genetic algorithm in optimization of mobile phase composition in high performance liquid chromatography].

Referring to traditional optimal methods, a method for the optimization of isocratic elution mobile phase composition in high performance liquid chromatography has been developed. In this method, the genetic algorithm based on line-crossover and plane-mutation is used. The principle of genetic algorithm and the process of optimization of mobile phase composition in reversed-phase ion-pair high performance liquid chromatography using genetic algorithm are introduced in details.

[Fast optimization of stepwise gradient conditions for ternary mobile phase in reversed-phase high performance liquid chromatography].

In high performance liquid chromatography, it is necessary to apply multi-composition gradient elution for the separation of complex samples such as environmental and biological samples. Multivariate stepwise gradient elution is one of the most efficient elution modes, because it combines the high selectivity of multi-composition mobile phase and shorter analysis time of gradient elution. In practical separations, the separation selectivity of samples can be effectively adjusted by using ternary mobile phase.