Improved analysis HPLC-ESI/triple method for mapping the methotrexate by mass spectrometry.

Methotrexate is a commonly utilized agent in pediatric oncology therapy. However, significant interindividual variability in its clearance can lead to delayed clearance and resultant severe toxicity. This underscores the urgent need for efficient and sensitive analytical methods to ensure patient safety.

ASFV infection induces lipid metabolic disturbances and promotes viral replication.

Introduction: African swine fever is a highly transmissible and lethal infectious disease caused by the African swine fever virus (ASFV), which has considerably impacted the global swine industry. Lipid metabolism plays a vital role in sustaining lipid and energy homeostasis within cells and influences the viral life cycle.

Methods And Results: In this study, we found that ASFV infection disrupts lipid metabolism in the host.

Suspension height tune-up with constant stiffness properties through motor-driving double-gas-chamber hydro-pneumatic strut: Experimental study and modeling.

In vehicle suspension, it is important to achieve continuous height adjustment to reduce the possibility of unstable and off-tracking caused by uneven postures. It is usually solved by air suspension, in which the dynamic properties will change under the adjusting process and these changes are not conducive to control. Considering the above, in this paper a double-gas-chamber hydro-pneumatic strut (DHPS) with the constant and/or predicted stiffness during continuous height adjustment, as masses (oil and gas) conversation are guaranteed in the whole system, which is achieved by volume variation of auxiliary gas chamber through motor-driving piston, is proposed.

Decellularized liver scaffolds for constructing drug-metabolically functional human liver models.

The creation of human liver models has long been a critical objective in academic, clinical, and pharmaceutical research, particularly for drug development, where accurate evaluation of hepatic metabolic dynamics is crucial. We have developed a bioengineered, perfused, organ-level human liver model that accurately replicates key liver functions, including metabolic activities, and protein synthesis, thus addressing some of the limitations associated with traditional liver monolayers, organoids, and matrix-embedded liver cells. Our approach utilizes liver-specific biomatrix scaffolds, prepared using an innovative protocol and fortified with matrix components that facilitate cellular interactions.

A review on triterpenoid and triterpenoid saponins from Xanthoceras sorbifolium Bung.

Xanthoceras sorbifolium Bunge, also known as Tu-Mu-Gua and Wen-Dan-Ge-Zi, has several applications. Clinical data and experimental studies have shown anti-tumor, anti-inflammatory, anti-bacterial, and anti-oxidant properties of Xanthoceras sorbifolium Bunge that inhibits prostate hyperplasia, lowers blood pressure and lipid level, and treats enuresis and urinary incontinence. It also has neuroprotective effects and can treat Alzheimer's disease and Parkinson's syndrome.

Tradeoff analysis between time cost and energy cost for fixed-time synchronization of discontinuous neural networks.

This article focuses on the tradeoff analysis between time and energy costs for fixed-time synchronization (FXTS) of discontinuous neural networks (DNNs) with time-varying delays and mismatched parameters. First, a more comprehensive lemma is systematically established to study fixed-time stability, which is less conservative than those in most current results. Besides, theoretical proof has proven that the upper bounds of the settling time (ST) in this article are more accurate compared to existing results.

Heavy metal contamination in Shanghai agricultural soil.

As heavy metals in soil could enrich in biomass and pose health risk to human, it is vital to monitor their contaminations to ensure qualified agricultural production. In this study, we collected >4000 soil samples from agricultural fields in Shanghai during 2010∼2020, and unveiled heavy metal contamination status in this metropolitan. We found that although Shanghai has a long industrialization history, the heavy metal levels in agricultural soil are within safe ranges according to national standard.

Radiomics from dual-energy CT-derived iodine maps predict lymph node metastasis in head and neck squamous cell carcinoma.

Objective: To develop and validate an iodine maps-based radiomics nomogram for preoperatively predicting cervical lymph node metastasis (LNM) in head and neck squamous cell carcinoma (HNSCC).

Materials And Methods: A total of 278 patients who pathologically confirmed as HNSCC were retrospectively recruited from two medical centers between June 2012 and July 2022. The training set (n = 152) and internal set (n = 67) were randomly selected from medical center A, and the patients from medical center B were enrolled as the external set (n = 69).

Effective TME-related signature to predict prognosis of patients with head and neck squamous cell carcinoma.

The tumor microenvironment (TME) is crucial for the development of head and neck squamous cell carcinoma (HNSCC). However, the correlation of the characteristics of the TME and the prognosis of patients with HNSCC remains less known. In this study, we calculated the immune and stromal cell scores using the "estimate" R package.

Epicardial fat in patients with metabolic syndrome: A systematic review and meta-analysis.

Purpose: Abnormally increased epicardial fat appears to be associated with an additional risk of major adverse cardiovascular events (MACEs) in the context of metabolic syndrome (MetS). However, evidence on the relationship between epicardial fat volumes (EFVs), epicardial fat thickness (EFT) and MetS remains inconsistent.

Methods: Specific searches of electronic databases from 1 January 2000 to 31 October 2022 were independently performed by two researchers.

The impact of industrial land mismatch on carbon emissions in resource-based cities under environmental regulatory constraints-evidence from China.

Achieving carbon neutrality has become a global common goal. For China, to reach peak carbon emissions and long-term carbon neutrality, the transformation and development of resource-based cities are essential. This study uses data from 114 prefecture-level resource-based cities from 2008 to 2019 as a sample and empirically tests the impact of industrial land mismatch on carbon emissions using the fixed effects model.

Synchronization in Finite-Time of Delayed Fractional-Order Fully Complex-Valued Dynamical Networks via Non-Separation Method.

The finite-time synchronization (FNTS) problem for a class of delayed fractional-order fully complex-valued dynamic networks (FFCDNs) with internal delay and non-delayed and delayed couplings is studied by directly constructing Lyapunov functions instead of decomposing the original complex-valued networks into two real-valued networks. Firstly, a mixed delay fractional-order mathematical model is established for the first time as fully complex-valued, where the outer coupling matrices of the model are not restricted to be identical, symmetric, or irreducible. Secondly, to overcome the limitation of the use range of a single controller, two delay-dependent controllers are designed based on the complex-valued quadratic norm and the norm composed of its real and imaginary parts' absolute values, respectively, to improve the synchronization control efficiency.

Skeleton Synthesis of a Plant-Derived Radioprotective Alkaloid Born to Produce a Novel Fused Heterocycle.

Alkaloids are a material treasure bestowed on humans by nature owing to their numerous biological activities. Orychophragine D, an alkaloid isolated from the seeds of was identified as bearing a novel skeleton and proved to have an excellent radioprotective effect. Different from the common alkaloid structure, the main block of orychophragine D is constructed of an oxotriazine and an oxopiperazine, which are connected in parallel by a C-N bond.

Bio-inspired magnetic-driven folded diaphragm for biomimetic robot.

Functional soft materials, exhibiting multiple types of deformation, have shown their potential/abilities to achieve complicated biomimetic behaviors (soft robots). Inspired by the locomotion of earthworm, which is conducted through the contraction and stretching between body segments, this study proposes a type of one-piece-mold folded diaphragm, consisting of the structure of body segments with radial magnetization property, to achieve large 3D and bi-directional deformation with inside-volume change capability subjected to the low homogeneous magnetically driving field (40 mT). Moreover, the appearance based on the proposed magnetic-driven folded diaphragm is able to be easily customized to desired ones and then implanted into different untethered soft robotic systems as soft drivers.

Correlation analysis between extrusion process variables and quality of purslane leaf powder rice extrudates.

Extrusion has become one of the most popular techniques in food processing, and the process parameters are closely related to product quality. Purslane (Portulaca oleracea L.) can be used in medical and food products as a vegetable and herb.

High-content imaging of human hepatic spheroids for researching the mechanism of duloxetine-induced hepatotoxicity.

Duloxetine (DLX) has been approved for the successful treatment of psychiatric diseases, including major depressive disorder, diabetic neuropathy, fibromyalgia and generalized anxiety disorder. However, since the usage of DLX carries a manufacturer warning of hepatotoxicity given its implication in numerous cases of drug-induced liver injuries (DILI), it is not recommended for patients with chronic liver diseases. In our previous study, we developed an enhanced human-simulated hepatic spheroid (EHS) imaging model system for performing drug hepatotoxicity evaluation using the human hepatoma cell line HepaRG and the support of a pulverized liver biomatrix scaffold, which demonstrated much improved hepatic-specific functions.

Purification of total flavonoids from flowers with resin column chromatography and evaluation of antioxidant activities .

Purification of total flavonoids from flowers (GBF) extracts were studied using six resins. Adsorption-desorption experiments indicated that polyamide resin is the most suitable resin. The optimal purification process of total flavonoids of GBF was as follows: a loading concentration of 5.

Hepatic Organoid-Based High-Content Imaging Boosts Evaluation of Stereoisomerism-Dependent Hepatotoxicity of Stilbenes in Herbal Medicines.

The complexity of chemical components of herbal medicines often causes great barriers to toxicity research. In our previous study, we have found the critical divergent hepatotoxic potential of a pair of stilbene isomers in a famous traditional Chinese herb, (Heshouwu in Chinese). However, the high-throughput evaluation for such stereoisomerism-dependent hepatotoxicity is a critical challenge.

Super Dominant Pathobiontic Bacteria in the Nasopharyngeal Microbiota Cause Secondary Bacterial Infection in COVID-19 Patients.

Coronavirus disease 2019 (COVID-19) is a respiratory infectious disease responsible for many infections worldwide. Differences in respiratory microbiota may correlate with disease severity. Samples were collected from 20 severe and 51 mild COVID-19 patients.

Exponential synchronization for variable-order fractional discontinuous complex dynamical networks with short memory via impulsive control.

This paper considers the exponential synchronization issue for variable-order fractional complex dynamical networks (FCDNs) with short memory and derivative couplings via the impulsive control scheme, where dynamical nodes are modeled to be discontinuous. Firstly, the mathematics model with respect to variable-order fractional systems with short memory is established under the impulsive controller, in which the impulse strength is not only determined by the impulse control gain, but also the order of the control systems. Secondly, the exponential stability criterion for variable-order fractional systems with short memory is developed.

Supercritical CO extraction of fermented soybean lipids against erastin-induced ferroptosis in rat pheochromocytoma cells.

The optimum supercritical carbon dioxide (SC-CO) extraction of fermented soybean lipids (FSE-C) was as follows: 35 °C, 30 MPa, and 2.40 ± 0.19% moisture content using response surface methodology.

Cloning and expression analysis of DnMSI1 gene in orchid species Lindl.

WD40 repeat proteins, the homologs of yeast MSI1, are conserved in plants, participating in protein complexes and playing fundamental functions in plant development. Although several MSI1-like proteins have been cloned and characterized in plants, the roles of MSI1-like proteins in the biennial ornamental plant, Lindl, are still unclear. Here, we report the cloning of the gene from Lindl with RACE technology.