Oil body bound oleosin-rhFGF9 fusion protein expressed in safflower (Carthamus tinctorius L.) stimulates hair growth and wound healing in mice.

Background: Fibroblast growth factor 9 (FGF9) is a heparin-binding growth factor, secreted by both mesothelial and epithelial cells, which participates in hair follicle regeneration, wound healing, and bone development. A suitable source of recombinant human FGF9 (rhFGF9) is needed for research into potential clinical applications. We present that expression of oleosin-rhFGF9 fusion protein in safflower (Carthamus tinctorius L.

[Expression of oleosin-rhFGF9 fusion protein in Carthamus tinctorius and determination of hair regeneration and wound repair potential in mice].

The expression of fibroblast growth factor 9 (FGF9) recombinant fusion protein in Carthamus tinctorius was used to identify its effect on hair regrowth and wound repair system in mice, providing a basis for C. tinctorius as a plant bioreactor, and establishing a foundation for commercial applications of FGF9 fusion protein in hair regrowth and wound repair. The identified pOTBar-oleosin-rhFGF9 plasmid was transformed into Agrobacterium tumefaciens EHA105 by freeze-thaw method, and the oleosin-rhFGF9 gene was transformed into safflower leaves by A.

Novel SA@Ca/RCSPs core-shell structure nanofibers by electrospinning for wound dressings.

Therapeutic drugs remain of great significance for the absorption of wound blood, the closure of wounds and rapid wound healing. Hence, we propose a novel composite nanofiber membrane with the above characteristics as a wound healing material. We utilize the reaction of calcium ion and alginate gel, sodium alginate (SA) and skin peptides (RCSPs) extracted from discarded skin; these two natural substances were successfully used to prepare composite nanofibers by coaxial electrospinning.