Functional Characterization of Double Mutations T929I/K1774N in the Voltage-Gated Sodium Channel of (Bagnall) Related to Pyrethroid Resistance.

(Bagnall), the main pest on legume vegetables, is controlled by pyrethroids in the field. Field strains of resistant to pyrethroids were collected from three areas in Hainan Province (Haikou, Ledong, and Sanya City), and two mutations, T929I and K1774N, were detected in the voltage-gated sodium channel. In this study, the sodium channel in was first subcloned and successfully expressed in oocytes.

Sonic hedgehog signaling: Alternative splicing and pathogenic role in medulloblastoma.

Alternative splicing (AS) produces the different mRNA splicing bodies, which are then translated into multiple protein isoforms and participate in various biological functions. With a deeper understanding of alternative splicing through the study of transcriptomes using high-throughput sequencing-based methods, the correlation between aberrant AS and diseases triggered a great concern, especially abnormal AS and cancer. Medulloblastoma (MB) is an intracranial tumor in children.

Detection of Putative Mutation I873S in the Sodium Channel of (Bagnall) Which May Be Associated with Pyrethroid Resistance.

Pyrethroid resistance of thrips has been reported in many countries, and knockdown resistance () has been identified as a main mechanism against pyrethroids in many insects. To characterize pyrethroid resistance in from the Hainan Province of China, we conducted a biological assay and sequenced the voltage-gated sodium channel gene domain II from field populations. It showed high resistance to the pyrethroids for 2019 and 2020, in which LC to lambda-cyhalothrin of was 1683.

Functional Characterization of Knockdown Resistance Mutation L1014S in the German Cockroach, (Linnaeus).

The effectiveness of pyrethroid insecticides is seriously threatened by knockdown resistance (), which is induced in insects by inherited single-nucleotide polymorphisms in the voltage-gated sodium channel (VGSC) gene. VGSC's L1014F substitution results in the classic mutation, which is found in many pest species. Other substitutions of the L1014 locus, such as L1014S, L1014C, L1014W, and L1014H, were also reported.

HSP90β chaperoning SMURF1-mediated LATS proteasomal degradation in the regulation of bone formation.

Heat shock protein 90 (HSP90) molecular chaperone is responsible for the stabilization and biological activity of a diverse set of client proteins. We have previously demonstrated that inhibition of HSP90 by 17-Demethoxy-17-allyaminogeldanmycin (17-AAG) not only reverses the glucocorticoid-induced bone loss but also enhances the basal level of bone mass in mice. Here, we investigate the potential mechanism underlying HSP90-associated osteoblast differentiation and bone formation.

Unique post-translational modifications diversify the sodium channels in peach aphid (Myzus persicae [Sulzer]).

Background: Myzus persicae (Sulzer), a worldwide pest, has caused remarkable damage to agriculture. Among the various control methods, chemical control (especially pyrethroids) is most commonly used. The targets of pyrethroids are voltage-gated sodium channels (Na s).

Ellipsoidal Abstract and Illustrative Representations of Molecular Surfaces.

Molecular visualization is often challenged with rendering of large molecular structures in real time. The key to LOD (level-of-detail), a classical technology, lies in designing a series of hierarchical abstractions of protein. In the paper, we improved the smoothness of transition for these abstractions by constructing a complete binary tree of a protein.

Prediction of Enzyme Function Based on Three Parallel Deep CNN and Amino Acid Mutation.

During the past decade, due to the number of proteins in PDB database being increased gradually, traditional methods cannot better understand the function of newly discovered enzymes in chemical reactions. Computational models and protein feature representation for predicting enzymatic function are more important. Most of existing methods for predicting enzymatic function have used protein geometric structure or protein sequence alone.