Development of a Pre-Modification Strategy to Overcome Restriction-Modification Barriers and Enhance Genetic Engineering in for Nisin Biosynthesis.

Due to the barriers imposed by the restriction-modification (RM) system, Nisin-producing industrial strains of often encounter low transformation efficiency, which seriously hinders the widespread application of genetic engineering in non-model . Herein, we present a novel pre-modification strategy (PMS) coupled with optimized plasmid delivery systems designed to systematically evade RM barriers and substantially improve Nisin biosynthesis in . Through the use of engineered strains with methylation profiles specifically optimized for C20, we have effectively evaded RM barriers, thereby facilitating the efficient introduction of large Nisin biosynthetic gene clusters into .

Complementary benefits of multivariate and hierarchical models for identifying individual differences in cognitive control.

Understanding individual differences in cognitive control is a central goal in psychology and neuroscience. Reliably measuring these differences, however, has proven extremely challenging, at least when using standard measures in cognitive neuroscience such as response times or task-based fMRI activity. While prior work has pinpointed the source of the issue-the vast amount of cross-trial variability within these measures-solutions remain elusive.

Regulation pattern of carotenoid biosynthesis induced by low light stress in Dunaliella.

This research aims to explore the response mechanism of carotenoid synthesis in Dunaliella bardawil under low light stress (≤13.5 µmol·m·s). We analyzed carotenoid composition and its correlation with carotenogenic genes (CRTs) differential expression, promoter regulatory elements and transcription factors.

Role of insulin-like growth factor-2 in Alzheimer's disease induced memory impairment and underlying mechanisms.

Alzheimer's disease (AD) is the most prevalent type of dementia. Treatments for AD do not reverse the loss of brain function; rather, they decrease the rate of cognitive deterioration. Current treatments are ineffective in part because they do not address neurotrophic mechanisms, which are believed to be critical for functional recovery.

Defects Calculation and Accelerated Interfacial Charge Transfer in a Photoactive MOF-Based Heterojunction.

Photocatalytic hydrogen production is currently considered a clean and sustainable route to meet the energy and environmental issues. Among, heterojunction photocatalysts have been developed to improve their photocatalytic efficiency. Defect engineering of heterojunction photocatalysts is attractive due to it can perform as electron trap and change the band structure to optimize the interfacial separation rate of photogenerated electron-hole pairs.

Neurosurgical Intervention in Primary Intraventricular Hemorrhage : Experience from a Center in China.

Objective: Primary intraventricular hemorrhage (PIVH) is a rare type of neurologic disorder and remains a challenge for cerebrovascular surgeons. This study intended to investigate the factors associated with neurosurgical intervention and its impact on outcome after PIVH.

Methods: We retrospectively included consecutive patients with PIVH admitted to at a single tertiary academic medical center in China.

Fyn, an important molecule in the brain, is a potential therapeutic target for brain tumours.

Under normal physiological conditions, Fyn, a nonreceptor tyrosine kinase, is involved in signal transduction pathways in the nervous system and in the formation and activation of T lymphocytes. Fyn is a member of the Src family of kinases (SFKs) and plays a role in cell morphogenic transformation, motility, proliferation, and death, which in turn influences the development and progression of various cancer types. SFKs are overexpressed or hyperactive in tumours, and they are engaged in several signalling pathways that lead to tumour development.

Knockout of B2M in combination with PD-L1 overexpression protects MSC-derived new islet β cells from graft rejection in the treatment of canine diabetes mellitus.

Background: The immunogenicity of allogeneic mesenchymal stem cells (MSCs) is significantly enhanced after transplantation or differentiation, and these cells can be recognized and cleared by recipient immune cells. Graft rejection has become a major obstacle to improving the therapeutic effect of allogeneic MSCs or, after their differentiation, transplantation in the treatment of diabetes and other diseases. Solving this problem is helpful for prolonging the time that cells play a role in the recipient body and for significantly improving the clinical therapeutic effect.

[Identification and functional analysis of the transcriptional factor GeERF4B-1 in ].

, a vine plant of Loganiaceae, has both medicinal and forage values. However, it is susceptible to low temperatures during growth. Exploring low temperature response genes is of great significance for cold resistance breeding of .

On the control of recurrent neural networks using constant inputs.

This paper investigates the controllability properties of a general class of recurrent neural networks that are widely used for hypothesis generation in theoretical neuroscience, including the modeling of large-scale human brain dynamics. Our study focuses on the control synthesis of such networks using constant and piecewise constant inputs, motivated by emerging applications in non-invasive neurostimulation such as transcranial direct current stimulation (tDCS). The neural network model considered is a continuous Hopfield-type system with nonlinear activation functions and arbitrary input matrices representing interactions among multiple brain regions.

RREB1 could act as an immunological and prognostic biomarker: From comprehensive analysis to osteosarcoma validation.

Background: The Ras-responsive element binding protein 1 (RREB1) is a transcription factor involved in various biological processes. Notably, RREB1 plays a role in tumor immunity by regulating tumor-related gene expression, shaping the tumor microenvironment, and modulating immune checkpoints. Given these functions, RREB1 has emerged as a potential regulatory target in tumor immunotherapy.

Changes in immune status of circulating NK cells in patients with latent tuberculosis infection.

Introduction: The presence of patients with latent tuberculosis infection (LTBI) has fueled the tuberculosis pandemic. We aimed to investigate the immune status of NK cells in LTBI patients.

Material And Methods: Twenty-one LTBI patients, 25 active pulmonary tuberculosis (APTB) patients and 25 healthy controls (HCs) participated in our research.

Outcome of adolescents and young adult acute myeloid leukemia patients compared with middle-aged patients: A single centre retrospective experience.

Adult acute myeloid leukemia (AML) patients under the age of 60 often receive similar intensive treatments, while outcomes between the adolescent and young adult (AYA) age group (18-39) and middle-aged adults (40-60 years) were seldom reported. We aim to study the characteristics and outcomes of AYA patients in comparison to middle-aged adults. A retrospective analysis was performed on AYA patients treated at Princess Margaret Cancer Center between 2008 and 2018.

Bibliometric analysis of global research trends in magnetic resonance imaging studies of substantia nigra in Parkinson's disease (2001-2024).

Background: Parkinson's disease (PD) is a globally prevalent neurodegenerative disorder, primarily characterized by muscle rigidity, resting tremor, and bradykinesia. The incidence of PD is rapidly escalating worldwide. Numerous studies have been conducted on the application of magnetic resonance imaging (MRI) in investigating the substantia nigra (SN) in PD patients.

Automatic recognition system for concrete cracks with support vector machine based on crack features.

Cracks are a common problem in concrete surfaces. With the continuous optimization of machine vision-based inspection systems, effective crack detection and recognition is the core of the entire system. In this study, support vector machine (SVM) was used to distinguish cracks from other regions.

Associations between PM, ambient heat exposure and congenital hydronephrosis in southeastern China.

Objectives: This research aims to analyze how exposure to fine particulate matter (PM5) and ambient heat during pregnancy increases the risk of congenital hydronephrosis (CH) in newborns.

Methods: A case-control study was conducted to investigate the relationship between exposure to PM and ambient heat during pregnancy and the occurrence of CH in newborns. The study, which was conducted from 2015 to 2020, included 409 infants with CH as the case group and 409 infants without any abnormalities as the control group.

Air pollutants, genetic susceptibility and the risk of schizophrenia: large prospective study.

Background: Evidence linking air pollutants and the risk of schizophrenia remains limited and inconsistent, and no studies have investigated the joint effect of air pollutant exposure and genetic factors on schizophrenia risk.

Aims: To investigate how exposure to air pollution affects schizophrenia risk and the potential effect modification of genetic susceptibility.

Method: Our study was conducted using data on 485 288 participants from the UK Biobank.

Naoxintong capsule for treating cardiovascular and cerebrovascular diseases: from bench to bedside.

Naoxintong Capsule (NXT), a renowned traditional Chinese medicine (TCM) formulation, has been broadly applied in China for more than 30 years. Over decades, accumulating evidences have proven satisfactory efficacy and safety of NXT in treating cardiovascular and cerebrovascular diseases (CCVD). Studies have been conducted unceasingly, while this growing latest knowledge of NXT has not yet been interpreted properly and summarized comprehensively.

3D Models of Sarcomas: The Next-generation Tool for Personalized Medicine.

Sarcoma is a complex and heterogeneous cancer that has been difficult to study in vitro. While two-dimensional (2D) cell cultures and mouse models have been the dominant research tools, three-dimensional (3D) culture systems such as organoids have emerged as promising alternatives. In this review, we discuss recent developments in sarcoma organoid culture, with a focus on their potential as tools for drug screening and biobanking.

A Novel Machine Learning Model for Predicting Stroke-Associated Pneumonia After Spontaneous Intracerebral Hemorrhage.

Background: Pneumonia is one of the most common complications after spontaneous intracerebral hemorrhage (sICH), i.e., stroke-associated pneumonia (SAP).

Novel erythrocyte-shaped electrosprayed nanoparticles for co-delivery of paclitaxel and osimertinib: Preparation, characterization, and evaluation.

In this work, novel erythrocyte-shaped electrosprayed nanoparticles (EENPs) were designed and constructed by tri-axial electrospraying technique with PEG as the outer layer, PLGA as the middle drugs (paclitaxel [PTX] and osimertinib [OSI]) carrier layer and air as the inner layer. The prepared EENP were characterized and evaluated based on their spectral and morphological attributes. After the PTX/OSI ratio and process optimization, the EENP has inspiring features, including nanoscale size, erythrocyte morphology with a concave disk shape, and satisfactory drug loading (DL) and encapsulation efficiency (EE).