Development of a novel recessive genetic male sterility system for hybrid seed production in maize and other cross-pollinating crops.

We have developed a novel hybridization platform that utilizes nuclear male sterility to produce hybrids in maize and other cross-pollinating crops. A key component of this platform is a process termed Seed Production Technology (SPT). This process incorporates a transgenic SPT maintainer line capable of propagating nontransgenic nuclear male-sterile lines for use as female parents in hybrid production.

[Increased level of prostate-specific antigen: a diagnostic marker of hyperandrogenism women].

Objective: To determine whether the increased level of serum prostate-specific antigen (PSA) could be used as a diagnostic marker of hyperandrogenism in women.

Methods: Forty-five female patients with hyperandrogenism and 50 healthy control women were detected for the levels of serum PSA, testosterone (T), sex hormone binding globulin (SHBG) and dehydroepiandrosterone sulfate (DHEA-S). The results were statistically analyzed.

[Development of radioimmunoassay for human sperm protein 17].

Objective: To develop a method of radioimmunoassay for human sperm protein 17(Sp17) determination.

Methods: Anti-recombinant human Sp17 antibody was prepared, the labeling of 125I-rhSp17 performed by chloramine T method, and radioimmunoassay of Sp17 developed.

Results: The assay range was 3.

[Plasma endothelin-1 in patients with prostate cancer and its clinical significance].

Objective: To investigate plasma endothelin-1 (ET-1) level in patients with prostate cancers and its clinical significance.

Methods: Plasma ET-1 level was measured by radioimmunoassay in 31 patients of prostate cancer (23 with non-HRPC, 8 with HRPC) and 26 patients of BPH.

Results: Compared with each other of the ET-1 level, there were no significant difference among the BPH group,non-HRPC group and HRPC group.

A chimeric ecdysone receptor facilitates methoxyfenozide-dependent restoration of male fertility in ms45 maize.

A mutation in the maize Ms45 gene results in abortion of microspore development and a male-sterile phenotype. MS45 protein has been localized to the tapetum and maximally expressed in anthers at the early vacuolate stage of microspore development. Molecular complementation analysis determined that a transformed copy of the gene fully restored fertility to ms45 maize.