[Protective effects of salidroside on injury induced by hypoxia/hypoglycemia in cultured neurons].

Objective: To study the protective effects of salidroside on injury induced by hypoxia/hypoglycemia in cultured SH-SY5Y cell.

Method: Apoptosis and intracellular free calcium concentration ([Ca2+]i) were measured by flow cytometry, morphological changes and neuronal necrosis were observed with fluorescence microscope, and the lactic dehydrogenate (LDH) release was measured by LDH kits.

Result: Hypoxia/hypoglycemia cultures for 2 hours induced neuronal apoptosis and necrosis.