Gut Microbiota Diversity and Overweight/Obesity in Infancy: Results from a Nested Case-control Study.

Objective: Childhood obesity is a major health concern worldwide. Previous studies have explored the relationship between obesity and gut microbiota. However, the results from such studies remain contradictory.

Cholinergic activation suppresses palmitate-induced macrophage activation and improves acylation stimulating protein resistance in co-cultured adipocytes.

Acylation-stimulating protein (ASP), produced through activation of the alternative complement immune system, modulates lipid metabolism. Using a trans-well co-culture cell model, the mitigating role of α7-nicotinic acetylcholine receptor (α7nAChR)-mediated cholinergic pathway on ASP resistance was evaluated. ASP signaling in adipocytes via its receptor C5L2 and signaling intermediates Gαq, Gβ, phosphorylated protein kinase C-α, and protein kinase C-ζ were markedly suppressed in the presence of TNFα or medium from palmitate-treated RAW264.

[Therapeutic effect of hemin on gestational hypertension in rats and the mechanism].

Objective: To investigate the therapeutic effects of hemin, an inducer of heme oxygenase, in a rat model of gestational hypertension and explore the possible mechanism.

Methods: Eighteen pregnant SD rats at day 12 of gestation were randomized equally into gestational hypertension model group, hemin treatment group, and normal pregnancy (control) group. In the former two groups, the rats were subjected to daily nitro-L-arginine methyl ester (L-NAME, 80 mg/kg) gavage since gestational day 14 for 7 consecutive days to induce gestational hypertension; saline was administered in the same manner in the control rats.

Diagnostic performance of contrast-enhanced sonography and acoustic radiation force impulse imaging in solid liver lesions.

Objectives: The purpose of this study was to evaluate the diagnostic performance of contrast-enhanced sonography and acoustic radiation force impulse imaging (ARFI) in differential diagnosis of benign and malignant liver lesions.

Methods: A total of 156 patients with liver lesions were included in the study. The images from conventional sonography and contrast-enhanced sonography and the shear wave velocity values of the liver lesions were obtained.

[Multicenter randomized clinical study of high-intensity focused ultrasound and cortical hormone in the treatment of non-neoplastic epithelial disorders of vulva].

Objective: To evaluate the efficacy of high-intensity focused ultrasound (HIFU) and cortical hormone in the treatment of non-neoplastic epithelial disorders of vulva.

Methods: A total of 268 cases with pathologically diagnosed non-neoplastic epithelial disorders of vulva were randomly allocated into two groups of high-intensity focused ultrasound (HIFU) (n = 119) and cortical hormone (n = 124). And 25 cases became lost to follow-ups.

Elevated visfatin levels in obese children are related to proinflammatory factors.

Background: Obesity and related metabolic diseases are associated with a state of chronic low-grade inflammation,which is characterized by abnormal cytokine production and increased synthesis of proinflammatory proteins.Recent studies have indicated that visfatin is both an adipokine and an inflammatory cytokine.

Objective: In this study, we assessed the association between serum visfatin concentrations and markers of systemic inflammation [high-sensitivity C-reactive protein (hs-CRP), interleukin 6 (IL-6), and tumor necrosis factor a (TNF-a)] and insulin resistance in Chinese obese children.

[Investigation on the prevalence of high risk human papillomavirus and cervical cancer among adult women, in Shenzhen].

Objective: To investigate the prevalence of high risk human papillomavirus (HPV) genital infection and cervical cancer in adult women from Shenzhen.

Methods: Cluster sampling was used to investigate the prevalence of HPV infection and cervical cancer from women aged 20 - 59 years old living in Luohu, Futian, Nanshan, Longgang and Baoan districts in Shenzhen from April 2006 to April 2010. All women were detected for liquid-based cytology test (LCT) or Thinprep cytologic test (TCT) and high-risk HPV-DNA test with hybrid capture II (HC-II).

Usefulness of acoustic radiation force impulse imaging in the differential diagnosis of benign and malignant liver lesions.

Rationale And Objectives: Acoustic radiation force impulse imaging (ARFI) is a newly developed technique for the evaluation of tissue stiffness. It has been applied to evaluate liver fibrosis, but only limited data for liver lesions are available. The aim of this study was to evaluate the tissue stiffness of liver lesions by using ARFI to differentiate benign from malignant liver lesions.

Enhanced ablation of high intensity focused ultrasound with microbubbles: an experimental study on rabbit hepatic VX2 tumors.

Purpose: This study was designed to assess the enhanced effect of high intensity focused ultrasound (HIFU) ablation with microbubbles on rabbit hepatic VX2 tumors and to compare the detection sensitivity of CEUS and CECT to determine the residual viable tissue after ablation of HIFU.

Methods: Forty rabbits with hepatic VX2 tumors were randomly separated into two groups (20 animals per group) before HIFU ablation. A bolus of 0.

[Prevalence of high-risk human papillomavirus and incidence of cervical intraepithelial neoplasia in female populations in Shenzhen, Guangdong Province].

Objective: To investigate the prevalence of high-risk human papillomavirus (HPV) and incidence of cervical intraepithelial neoplasia (CIN) in female populations in Shenzhen, Guangdong Province, China.

Methods: Totally 1137 women aged 15-59 from Shahe Community, Nanshan District, Shenzhen were investigated for cervical cancer during an population-based epidemiological screening from November 2004 to December 2004. Visual inspection with acetic acid (VIA), colposcopy, liquid-based cytology test (LCT), and hybrid capture 2 (HC-) were performed to detect the high-risk HPV types in cervical secretions.

[Role of cytological test for cervices to identify human papillomavirus in the screening program on serious cervical lesion].

Objective: To evaluate the value of cervical cytology and hybrid capture II (HC- II) human papillomavarus (HPV) test for screening cervical lesion.

Methods: Conventional papanicolaou (Pap) smear by improved take-samples, liquid-based cytology test (LCT) and HC- II human papillomavarus test were performed in 425 women in Shang Mei-lin community of Futian region in Shenzhen city, from December 2007 to March 2008 and the above methods were performed in 75 women in Shenzhen Hospital of Peking University at the same time. Age stratified sampling was used.

[Role of progesterone in acylation stimulating protein-receptor C5L2 pathway in adipocytes and preadipocytes].

Objective: To evaluate the effects of progesterone on the mRNA expression of acylation stimulating protein (ASP)-receptor C5L2 in adipocytes and preadipocytes and the C5L2 protein expression on the cell surface.

Methods: Preadipocytes of the line 3T3-L1 were cultured and induced to differentiate. Progesterone of the doses 0 - 1 x 10(-6) mol/L was added into the cultured fluid of the mature 3T3-L1 adipocytes and preadipocytes overnight.

[Experimental study of portal hemodynamics after microcapsule transplantation by intraportal and transarterial approach].

Objective: To compare the changes of portal hemodynamics after microcapsule transplantation by intraportal and transarterial approach with color doppler sonography.

Methods: Thirty male dogs were divided into 2 groups randomly: Group PV (microcapsules implanted into the portal vein) and Group HA (microcapsules implanted into the hepatic artery). Both groups were divided into 3 subgroups(PV1, PV2, and PV3; HA1, HA2, and HA3) according to the number of implanted microcapsules (8,000/kg, 16,000/kg, and 32,000/kg).

[Preparation and examination of the vitro releasing of pH-dependent kuijiekang tablet for colon-specific delivery].

Objective: To prepare the pH-dependent kuijiekang tablet for colon-specific delivery used in treating the ulcerative colitis and to develop the in vitro release method of kuijiekang tablets.

Methods: The coating prescription was screened by the in vitro delivery of Puerarin and Berberine hydrochloride. The in-vitro releasing property of the preparation was examined by the method of in-vitro delivery.

[Contrast enhanced power Doppler in evaluating the angiogenesis in hepatocellular carcinoma].

Objective: To evaluate the role of contrast enhanced power Doppler in evaluating tumor angiogenetic activity.

Methods: Thirty-two patients with hepatocellular carcinoma were analysed. Flow signals of hepatocellular carcinoma were observed by power Doppler imaging after the injection of contrast agent, and then the relative perfusion rate and blood flow were assessed.

Childhood gynecomastia: a clinical analysis of 240 cases.

Two hundred forty cases of childhood gynecomastia were studied retrospectively. There were 13 cases aged 3 to 10 years and 227 cases aged 11 to 15 years. Of the 240 cases of gynecomastia, 160 presented with bilateral breast enlargement, 50, left breast enlargement, and 30, right breast enlargement.

[Determination of tanshinone II A and deoxyschizandrin in danshenwuweizi tablet by RP-HPLC].

Objective: to set up a determination of tanshinone II A and deoxyschizaadrin in danshenwuweizi tablet by RP-HPLC.

Methods: two components were separated through Kromasil C18 (250 mm x 4.6 mm, 5 microm) column with methanol-water (85:15) by volume as a mobile phase.

[Comparison of treatment response in primary hepatocellular carcinomas with contrast-enhanced ultrasonography and contrast-enhanced helical CT].

Objective: To investigate the value of contrast-enhanced ultra sonography for non-surgical treatment response in hepatocellular carcinomas.

Methods: Non-surgical therapies were performed on 56 patients (64 liver neoplasms) who were diagnosed by ultrasonography-guided biopsy before the therapy. Contrast-enhanced ultrasonography(CEUS) and contrast-enhanced helical CT were performed to assess the treatment response.

Endoscopic variceal ligation combined with Hassab's procedure in preventing the recurrence of esophageal varices.

Objective: To assess the efficacy of endoscopic variceal ligation (EVL) combined with Hassab's procedure in the prevention of variceal recurrence.

Methods: One hundred and thirty-five patients with esophageal varices were randomized to receive EVL alone, Hassab's procedure alone or a combination of EVL and Hassab's procedure for variceal eradication. Ultrasonographic venous network images were recorded by an esophageal microprobe before and after the EVL or Hassab's procedure.

[Distribution of human papillomavirus types in Shenzhen women].

Objective: To investigate the status of genital infection as well as distribution of types of human papillomavirus (HPV) in women in Shenzhen and provide population data for the future vaccine intervention on cervical cancer.

Methods: Women with age between 15 and 59 years were selected in cluster stratified sampling from Huaqiaocheng community, Nanshan district, Shenzhen and received a population-based cervical cancer screening. After consent, every woman was interviewed by using questionnaire and tested by liquid-based cytology and HPV DNA (hybrid capture 2 and gene chips typing) separately.

[Comparison of ultrasound-guided mammotome and Tru-cut biopsy needle in diagnosing breast masses].

Objective: To compare the diagnostic value of ultrasound-guided mammotome and Tru-cut biopsy needle in diagnosing breast masses.

Methods: Retrospective analysis was performed in 214 patients with breast masses obtained separately by mammotome or Tru-cut biopsy needle guided by ultrasound, and analyzed by pathological examination. The success ratios of sample choosing and the coincident ratios of pathological diagnosis were compared.