Publications by authors named "Rui-Ying Zhu"
Article Synopsis
- - The study investigates how nitric oxide (NO) enhances polysaccharide production in Nostoc flagelliforme through a process called S-nitrosylation (SNO), which modifies certain enzymes involved in this biosynthesis.
- - It was found that certain key enzymes (G6PDH, ICDH, and UGDH) have their activity correlated with NO levels, and specific enzymes (UGDH and G6PDH) are particularly affected by SNO, as shown through various laboratory techniques.
- - The research identifies specific sites on the enzymes that are modified by NO and proposes that this mechanism could lead to improved industrial production of polysaccharides from Nostoc flagelliforme.
Importance: Preclinical studies suggest that amylin has a U-shaped dose-response association with risk of Alzheimer disease (AD). The association of plasma amylin with AD in humans is unknown.
Objectives: To measure amylin concentration in plasma by using enzyme-linked immunosorbent assay and to study the association between plasma amylin, incidence of AD, and brain structure in humans.
The power of synthetic biology has enabled the expression of heterologous pathways in cells, as well as genome-scale synthesis projects. The complexity of biological networks makes rational de novo design a grand challenge. Introducing features that confer genetic flexibility is a powerful strategy for downstream engineering.
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- - Debugging genome sequences is critical for creating synthetic genomes, and a synthetic yeast chromosome (synX) was synthesized with 707,459 base pairs, showing good functionality under diverse conditions.
- - The pooled PCRTag mapping (PoPM) technique was developed to detect genetic changes that impact cell fitness, enabling researchers to identify and correct various "bugs."
- - Notable corrections addressed complex gene amplifications, a growth defect related to a recoded sequence, and issues affecting promoter function, demonstrating PoPM's effectiveness in synthetic genome debugging and phenotype-genotype mapping.
Human immunodeficiency virus, type 1 (HIV-1) replication requires the interaction of Tat protein with the human cyclinT1 (hCyclinT1) subunit of the positive transcription elongation factor (P-TEFb) complex, which then cooperatively binds to transactivation response element (TAR) RNA to transactivate HIV transcription. In this report, a non-immune human single-chain antibody (sFv) phage display library was used to isolate anti-hCyclinT1 sFvs that could disrupt hCyclinT1-Tat interactions. The N-terminal 272 residues of hCyclinT1, including the entire cyclin domains and the Tat.
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