Publications by authors named "Rui-Song Huang"
Background: While DNA barcoding is an important technology for the authentication of the botanical origins of Chinese medicines, the suitable markers for DNA barcoding of the genus Uncaria have not been reported yet. This study aims to determine suitable markers for DNA barcoding of the genus Uncaria (Gouteng).
Methods: Genomic DNA was extracted from the freshly dried leaves of Uncaria plants by a Bioteke's Plant Genomic DNA Extraction Kit.
Objective: To study the qualitative and quantitative determination of Zhuang medicine Jasmini Sambacis Flos and establish its quality control standard.
Methods: Macroscopic, microscopic and TLC identification were adopted to carry out the qualitative identification, the mensuration of inspection items and extractum were according to the Chinese Pharmacopoeia. The contents of quercetin and kaempferide in Jasmini Sambacis Flos were determined by HPLC.
[Research on quality standards of zhuang medicine Lonicerae dasystylae flos].
Zhongguo Zhong Yao Za Zhi
March 2013
Objective: To establish quality standard of Zhuang medicine Lonicera dasystyla, and provide scientific basis for the quality control of L. dasystyla.
Method: Characteristics of materia medica, microscopic features, TLC indentification, inspection, extractum and determination of chlorogenic acid, macranthoidin B, dipsacoside B were carried out through the experience, microscopic, physical and chemical methods, respectively.
Objective: To establish TLC scanning method for the determination of camptothecin in Camptotheca acuminata fruit, and analysis the dynamic accumulation of camptothecin in Camptotheca acuminata fruit to find out the best collection period.
Methods: Silica gel H-CMC-Na thin layer plate was adopted in the determination with chloroform-acetone (7 : 3) used as deeloper, Single-wavelength and linear scanning of TLC was used, and the detection wavelength was 360 nm.
Results: There was a good linear relationship for Comptothecin within the range of 0.
[Studies on fingerprints of ant nest of Macrotermes annadalei from different areas].
Zhongguo Zhong Yao Za Zhi
November 2005
Objective: To study comparatively the fingerprint of zhuang medicinal material-ant nest of Macrotermes annadalei from different areas in Guangxi.
Method: The fingerprint of total amino acid of 12 lots of ant nest of M. annadalei from different areas was determined by high speed amino acid analyzer.
Objective: To establish the method for quality control of Xingnao tinctures.
Method: The Mentholum, Flos Caryophylli and Borneoum Syntheticum in this medicine were identified by TLC method. The content of Mentholum was determined by GC.