[Effects of exogenerous nucleotides on the apoptosis of intestinal epithelial cells IEC-6].

Objective: To investigate the effects of exogenous nucleotides on apoptosis of a normal rat small intestinal epithelial cell line, IEC-6.

Methods: Cultured IEC-6 cells were treated by four kinds of monophosphate nucleotides and their mixture prepared according to their composition in human milk, then the cell apoptosis was determined by flow cytometry measurement, morphologic characterization, and electron-microscope observation.

Results: IEC-6 cells treated with AMP or GMP showed a apotosis peak in flow cytometry measurement, but only AMP produce typical apoptosis characteristics in electron-microscope observation.

[Sensitivity comparison of DNA damage and repair capacity in TK6 and WTK1 cells].

Objective: Explore the mechanisms of DNA damage and repair capacity in TK6 and WTK1 cells that are derived from the same parental cell line WI-L2 (tk+/+) human diploid lymphoblastoid cells but are different in their p53 gene status.

Methods: The TK6 and WTK1 cells treated with H2O2 were detected by single cell gel electrophoresis. Then a comparison was made on the comet cell rate, comet cell tail length and p53 protein expression level between TK6 and WTK1.

Effects of fat-soluble extracts from vegetable powder and beta-carotene on proliferation and apoptosis of lung cancer cell YTMLC-90.

Objective: The aim of this investigation was to study the effects of fat-soluble extracts from vegetable powder (FEFVP) and beta-carotene on the proliferation and apoptosis of cultured YTMLC-90 lung cancer cells.

Methods: The lung cancer cells were continuously exposed to a broad range of concentration of FEFVP and beta-carotene. The proliferation was evaluated in MTT test.