In this study, to explore the effect of growth hormone changes on the related genes and regulatory roles of the turtle, PCR amplification, real-time fluorescence quantitative analysis, and enzyme cutting technology were used to clone and sequence the () gene, (), and () sequence of . The effects of human growth hormone on the mRNA expression of growth-axis-related genes , , and in different sexes were observed. The study of the gene in turtles using real-time fluorescence quantitative PCR showed that the gene was mainly expressed in the nervous system and the digestive system, with the highest expression found in the brain, while the gene and the gene were expressed in all tissues of .
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