Publications by authors named "Rui-Cong Hao"
[Generation and Evaluation of Human Umbilical Cord Derived Mesenchymal Stem Cells with Antioxidant Capacity].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Article Synopsis
- The study aimed to prepare antioxidant-capable mesenchymal stem cells (AO-MSC) from human umbilical cords and assess their biological properties.
- The AO-MSC were isolated using a method that involved allowing tissue debris to adhere to the culture surface, while control cells were obtained through traditional collagenase digestion.
- Results demonstrated that AO-MSC displayed higher levels of antioxidant substances and good self-renewal ability, but had a weaker capacity for adipogenic and osteogenic differentiation compared to conventional MSC.
Skeletal stem cells (SSC) have gained attentions as candidates for the treatment of osteoarthritis due to their osteochondrogenic capacity. However, the immunomodulatory properties of SSC, especially under delivery operations, have been largely ignored. In the study, we found that Pdpn and Grem1 SSC subpopulations owned immunoregulatory potential, and the single-cell RNA sequencing (scRNA-seq) data suggested that the mechanical activation of microgel carriers on SSC induced the generation of PdpnGrem1Ptgs2 SSC subpopulation, which was potent at suppressing macrophage inflammation.
View Article and Find Full Text PDFRecent investigations have shown that the necroptosis of tissue cells in joints is important in the development of osteoarthritis (OA). This study aimed to investigate the potential effects of exogenous skeletal stem cells (SSCs) on the necroptosis of subchondral osteoblasts in OA. Human SSCs and subchondral osteoblasts isolated from human tibia plateaus were used for Western blotting, real-time PCR, RNA sequencing, gene editing, and necroptosis detection assays.
View Article and Find Full Text PDFArticle Synopsis
- - The study investigates the potential of articular cartilage stem cells (ACSCs) in treating osteoarthritis (OA) by isolating these cells from diseased joints and evaluating their effects on joint health in rat models.
- - ACSCs demonstrated significant stem cell-like properties and improved joint conditions in OA rats, notably reducing abnormal bone remodeling after injection into the knee joints.
- - The results indicate that ACSCs inhibit the formation of osteoclasts (bone-resorbing cells) in the osteoarthritis context, mediated by specific proteins which contribute to joint healing and improved bone structure.
Article Synopsis
- Skeletal stem/progenitor cells (SSPCs) are found in bones and are essential for bone development, maintenance, and healing, but their diversity and regenerative abilities in mice need more research.
- This study uses advanced single-cell RNA sequencing to analyze SSPC populations in mouse bones and identifies various cell types involved in bone growth and regeneration.
- A new population of SSPCs named Cd168 was discovered, which shows strong regenerative potential and is found in specific areas of postnatal mouse bones, indicating its role in healing and tissue formation.
[Establishment and Evaluation of Intestinal Injury Model of Mouse Acute Graft Versus Host Disease Based on An Organoid Technology].
Zhongguo Shi Yan Xue Ye Xue Za Zhi
February 2023
Objective: To establish an intestinal organoid model that mimic acute graft versus host disease (aGVHD) caused intestinal injuries by using aGVHD murine model serum and organoid culture system, and explore the changes of aGVHD intestine in vitro by advantage of organoid technology.
Methods: 20-22 g female C57BL/6 mice and 20-22 g female BALB/c mice were used as donors and recipients for bone marrow transplantation, respectively. Within 4-6 h after receiving a lethal dose (8.
Background: Repairing radiation-induced bone injuries remains a significant challenge in the clinic, and few effective medicines are currently available. Psoralen is a principal bioactive component of Cullen corylifolium (L.) Medik and has been reported to have antitumor, anti-inflammatory, and pro-osteogenesis activities.
View Article and Find Full Text PDFBackground: Although increasing evidence has demonstrated that human dental pulp stem cells (hDPSCs) are efficacious for the clinical treatment of skeletal disorders, the underlying mechanisms remain incompletely understood. Osteoarthritis (OA) is one of the most common degenerative disorders in joints and is characterized by gradual and irreversible cartilaginous tissue damage. Notably, immune factors were newly identified to be closely related to OA development.
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