Heterologous expression, enzymatic properties, product analysis and molecular docking of assimilative nitrite reductase (NiR) in Bacillus velezensis GXMZU-B1 derived from mariculture.

High concentrations of nitrite stress aquatic animals, leading to significant fish and shrimp deaths as well as environmental pollution. Reducing nitrite levels in high-density aquaculture is crucial for both aquaculture safety and environmental protection. Nitrite reductase (NiR) can rapidly reduce nitrite in water, offering potential applications in aquaculture and water treatment.

Facile synthesis of high-swelling cyclodextrin polymer for sustainable water purification.

Porous materials are widely used in the adsorption field to sequester pollutants to address the global sustainable water security and water scarcity concerns. However, there are still challenges that limit their industrial application, especially the required rational design and construction of porous structures. Here, we report a high-swelling cyclodextrin polymer (His-CDP) that is facilely synthesized without additional design and templates, to achieve high affinity, non-specific and rapid adsorption of pollutants.

Adsorption properties and competitive adsorption mechanism exhibited by carbon-nanotube-modified biochar for removal of crude oil and Ni(II) pollutants from water.

Carbon-nanotube-modified biochar (CNT3-CBC) with a nanostructured surface was prepared by using cattle manure as the raw material via the impregnation method. This modified biochar was then used to adsorb petroleum and Ni(II) from aqueous solutions. Various physicochemical characterization techniques were employed, including SEM, BET analysis, FTIR, and XPS.

An ultrasensitive electrochemical sensor for the simultaneous detection of baicalin and baicalein in pharmaceuticals and serum samples.

Developing an accurate and ultrasensitive method for the simultaneous detection of baicalin and baicalein, two key flavonoid from Scutellaria baicalensis, is critical, particularly in the context of traditional Chinese medicine. This study focuses on an innovative electrochemical approach, utilizing a biochar (BC)/ZIF-67/MnCoO composite, which was fabricated via a combination of ultrasonic, calcination, and hydrothermal treatments. The resulting BC/ZIF-67/MnCoO electrode exhibits exceptional sensitivity, with linear response ranges of 0.

Adjuvant sorafenib for hepatocellular carcinoma after radiofrequency ablation versus radiofrequency ablation: analysis of its efficacy and safety.

Objectives: For the treatment of early hepatocellular carcinoma, we compared the efficacy and safety of radiofrequency ablation (RFA) alone and radiofrequency ablation combined with sorafenib (RFA+Sor).

Methods: A total of 164 patients with early HCC were included in the study. There were 87 patients who underwent RFA alone, and 77 patients who underwent RFA+Sor treatment.

Non-canonical function of PHGDH promotes HCC metastasis by interacting with METTL3.

Purpose: Phosphoglycerate dehydrogenase (PHGDH), a pivotal enzyme in serine synthesis, plays a key role in the malignant progression of tumors through both its metabolic activity and moonlight functions. This study aims to elucidate the non-canonical function of PHGDH in promoting hepatocellular carcinoma (HCC) metastasis through its interaction with methyltransferase-like 3 (METTL3), potentially uncovering a novel therapeutic target.

Methods: Western blot was used to study PHGDH expression changes under anoikis and cellular functional assays were employed to assess its role in HCC metastasis.

[Hereditary protein S deficiency in a patient with prominent mesenteric venous thrombosis: A case report].

Hereditary protein S deficiency (PSD) is an autosomal dominant disorder caused by mutations in the 1 gene which can cause venous thrombosis. Individuals with PSD usually present with recurrent deep vein thrombosis and/or pulmonary embolism, but thrombosis may occur at unusual sites, such as the mesenteric and portal veins. Here we report a case of hereditary protein S deficiency patient with predominant mesenteric venous thrombosis.

[Assessment of residual symptoms in patients with axial spondyloarthritis with low disease activity and analysis of its related factors].

Objective: To comprehensively assess the occurrence of residual symptoms in patients with axial spondyloarthritis who have successfully attained the treatment goal of low disease activity, and to conduct a thorough analysis of the related factors.

Methods: An analysis was performed on axial spondyloarthritis patients who achieved low disease activity for the first time during their visits at the Rheumatology and Immunology Department of Peking University Third Hospital, spanning from May 1, 2021, to February 29, 2024. Based on the ankylosing spondylitis disease activity score-C-reactive protein (ASDAS-CRP), the patients who achieved low disease activity were divided into a non-remission low disease activity group and a remission group.

Nondestructive Analysis of Commercial Batteries.

Electrochemical batteries play a crucial role for powering portable electronics, electric vehicles, large-scale electric grids, and future electric aircraft. However, key performance metrics such as energy density, charging speed, lifespan, and safety raise significant consumer concerns. Enhancing battery performance hinges on a deep understanding of their operational and degradation mechanisms, from material composition and electrode structure to large-scale pack integration, necessitating advanced characterization methods.

Percutaneous nephroscopy combined with ultrasound-guided negative-pressure suction for the treatment of perirenal hematoma.

Percutaneous renal puncture drainage is needed in patients with perirenal hematoma (PRH, renal around hematoma) with poor conservative treatment. Early and thorough removal of hematoma is closely related to the recovery of patients, but percutaneous renal drainage is not effective in some patients. The clinical data of 5 patients with PRH treated in the first affiliated Hospital of Chongqing Medical University from June 2020 to June 2024 were analyzed retrospectively.

Fresh-Cooked but Not Cold-Stored Millet Exhibited Remarkable Second Meal Effect Independent of Resistant Starch: A Randomized Crossover Trial.

It is well established that cold storage results in increased resistant starch and a reduced glycemic index in carbohydrate food. However, the effects of cold storage on the glycemic response of the second meal of cereals remain unclear. The aim of this study was to compare the postprandial glycemic responses between the paired glutinous and non-glutinous grains, either fresh-cooked or refrigerated, after both the first and second meals.

The Growth and Ion Absorption of Sesbania ( and Hairy Vetch () in Saline Soil Under Improvement Measures.

Soil salinization is a serious threat to the ecological environment and sustainable agricultural development in the arid regions of northwest China. Optimal soil salinization amelioration methods were eagerly explored under different soil salinity levels. Sesbania and hairy vetch are salt-tolerant plants, and green manure improved the saline environment.

Endovascular therapy for acute vertebrobasilar occlusion (VERITAS): a systematic review and individual patient data meta-analysis.

Background: Trials of endovascular therapy for basilar artery occlusion, including vertebral occlusion extending into the basilar artery, have shown inconsistent results. We aimed to pool data to estimate safety and efficacy and to explore the benefit across pre-specified subgroups through individual patient data meta-analysis.

Methods: VERITAS was a systematic review and meta-analysis that pooled patient-level data from trials that recruited patients with vertebrobasilar ischaemic stroke who were randomly assigned to treatment with either endovascular therapy or standard medical treatment alone.

A rapid, non-destructive, and accurate method for identifying citrus granulation using Raman spectroscopy and machine learning.

Citrus fruits are widely consumed for their nutritional value and taste; however, juice sac granulation during fruit storage poses a significant challenge to the citrus industry. This study used Raman spectroscopy coupled with machine learning algorithms to rapidly, non-destructively, and precisely detect citrus granulation. The investigation analyzed 969 Raman spectral data points, comprising 714 non-granulated and 255 granulated citrus samples.

Diels-Alder reaction in hydrogel synthesis: Mechanisms and functional aspects.

The Diels-Alder reaction, a classical (4+2) cycloaddition process, holds significant standing within the realms of organic synthesis and polymer chemistry, frequently employed in areas such as pharmaceutical production and material science. Recently, hydrogels constructed via Diels-Alder reactions have garnered considerable attention from researchers. This review aims to summarize the advancements in utilizing the Diels-Alder reaction for hydrogel synthesis, exploring its impact on structural design, functionalization, and application domains.

The significance of in multiple myeloma.

The treatment of multiple myeloma (MM) has significantly advanced; however, the underlying genetic mechanisms remain elusive. Clonal events and genetic alterations are recognized as pivotal in the pathogenesis of MM. It is now understood that a multitude of gene mutations, including those affecting RAS, , , and 1q21 amplification, are prevalent in this disease.

Multiomics reveals tumor microenvironment remodeling in locally advanced gastric and gastroesophageal junction cancer following neoadjuvant immunotherapy and chemotherapy.

Background: Perioperative chemotherapy is the standard of care for patients with locally advanced gastric and gastroesophageal junction cancer. Recent evidence demonstrated the addition of programmed cell death protein 1 (PD-1) inhibitors enhanced therapeutic efficacy. However, the mechanisms of response and resistance remain largely undefined.

Lead accumulation and concomitant reactive oxygen species (ROS) scavenging in Robinia pseudoacacia are dependent on nitrogen nutrition.

Heavy metal pollution combined with nitrogen (N) limitation is a major factor preventing revegetation of contaminated land. Woody N-fixing legumes are a natural choice for phytoremediation. However, the physiological responses of woody legumes to lead (Pb) with low N exposure are currently unknown.

Effect of inspiratory and expiratory muscle training on respiratory function and decannulation outcome in patients with tracheostomy after stroke: a randomized controlled trial.

Background: This study aimed to evaluate the clinical efficacy and safety of inspiratory and expiratory muscle training (IEMT) for patients who underwent tracheostomy after stroke.

Methods: The study was an investigator-initiated, single-center, two-arm, evaluator-blinded, randomized clinical trial conducted at West China Hospital of Sichuan University, China, from January 2022 to June 2022. The patients were randomly divided into the intervention group and control group.

Amelioration of myofibrillar protein emulsion gel properties by mildly oxidized sunflower oil.

Different oxidation states of oil affect the emulsion stablility and gel properties of emulsified meat products. Emulsified chicken gels were prepared using sunflower oil (SFO) with different oxidation levels (0, 20, 40, and 60 min, 120 °C). The pre-oxidation treatment of oils was investigated for emulsification and gelation of myofibrillar protein (MP).

Physicians and hospital pharmacists' knowledge, attitudes, and practices towards polypharmacy in older patients with chronic diseases.

This web-based cross-sectional study aimed to evaluate the knowledge, attitudes, and practices of physicians and hospital pharmacists towards polypharmacy in older adult patients with chronic diseases in China. This study enrolled 374 physicians and pharmacists (270 females, 92 physicians) in 20 Chinese provinces between December 2022 and March 2023. The knowledge, attitude, and practice scores were 12.