Helicobacter pylori eradication to prevent gastric cancer in a high-risk region of China: a randomized controlled trial.

Context: Although chronic Helicobacter pylori infection is associated with gastric cancer, the effect of H pylori treatment on prevention of gastric cancer development in chronic carriers is unknown.

Objective: To determine whether treatment of H pylori infection reduces the incidence of gastric cancer.

Design, Setting, And Participants: Prospective, randomized, placebo-controlled, population-based primary prevention study of 1630 healthy carriers of H pylori infection from Fujian Province, China, recruited in July 1994 and followed up until January 2002.

New pregnane glycosides from Stelmatocrypton khasianum.

Four new pregnane glycosides, stelmatocryptonoside A, B, C, and D (1-4), were isolated from the stems of Stelmatocrypton khasianum. On the basis of chemical and spectral data, the structures of 1-4 were established as 3beta, 16alpha-dihydroxy-pregn-5-en-20-one-16-O-beta-D-glucopyranosyl-(1-->2)-[beta-D-glucopyranosyl-(1-->6)]-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside; 3beta, 20-dihydroxy-pregn-5-en-20-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl-(1-->2)-beta-D-digitalopyranoside; 3beta, 16alpha-dihydroxy-pregn-5-en-20-one-16-O-beta-D-glucopyranosyl-(1-->2)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside; and 3beta, 16alpha-dihydroxy-pregn-5-en-20-one-16-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside. This is the first report of pregnane glycosides with sugar chains linked at C-16 of the aglycone.

Studies on the Effect of Recombinant Human Tumor Necrosis Factor-alpha on Leukemic Cell Lines by (31)P-NMR.

31P nuclear magnetic resonance (NMR) spectroscopy has been used for monitoring cellular metabolism in human lymphoma cell line Molt-4 and human promyelocyte leukemia cell line HL-60. (31)P-NMR spectra of Molt-4 cells and HL-60 cells consist primarily of peaks of phosphomonoesters, inorganic phosphate (Pi), diphosphodiesters and ATP. A decrease in the ATP/Pi ration occurred after treatment of the Molt-4 cells with recombinant human tumor necrosis factor-alpha(rhTHF-alpha).