Simultaneous measurement of phenols by three-way fluorescence spectroscopy: A comparison of N-PLS/RBL, U-PLS/RBL and PARAFAC.

Phenol, o-cresol, p-cresol, catechol and resorcinol are five phenolic compounds with extremely similar structure. Their fluorescence spectra are hard to be analyzed because of the serious spectral overlaps between any two of the five phenolic components in the mixture system. In this experiment, multi-dimensional partial least-squares (N-PLS), unfolded partial least-squares (U-PLS) with residual bilinearization (RBL) and parallel factor analysis (PARAFAC) are employed to analyze the three-way fluorescence spectra aiming to achieve quantitative results.

Study on Three-Dimensional Fluorescence Spectral Characteristics of Heterocyclic Pesticides in Different Environmental Conditions.

The impact analysis of different environments on the fluorescence emission spectrum of pesticides is critical in detecting the concentration of pesticides. In this paper, three kinds of pesticides, carbendazim, carbaryl and fuberidazole, were selected as the research objects. Under different environment, such as different pH values and the presence of different common anion or cation, three-dimensional fluorescence spectral emission (EEM) characteristic of pesticides were analyzed.

[Rapid Quantitative Detection of Bacterial in Water Based on Multi-Wavelength Scattering Spectra].

The structures of bacterial cells are analyzed in this paper. The scattering components of individual cell were divided into two parts including external structure and internal structure. The interpretation model of bacteria about scattering light is established.

[Laser Induced Time-Resolved Fluorescence Spectroscopy Characteristics of Fluoranthene in Ethanol with Eanosecond Laser of 355 nm].

Polycyclic Aromatic Hydrocarbons (PAHs) have been widely investigated in environmental field, for most of them are mutagenic (carcinogenic, teratogenic, mutagenic). The influence of delay time and gate width on the time-resolved fluorescence spectroscopy of fluoranthene in ethanol was studied in this paper. Furthermore, laser induced time-resolved fluorescence spectroscopy of fluoranthene with different concentration were also researched.

[Effect of Methanol-Water Mixture on Three-Dimensional Fluorescence Spectra of Carbaryl].

Extensive use of pesticides has a significant impact on the environment. Carbaryl, whose residues stay in the surface water, is one of the most widely used broad spectrum insecticides in agriculture. It is important to understand carbaryl spectral characteristics and detection methods.

[Study of Rapid Species Identification of Bacteria in Water].

Multi-wavelength ultraviolet visible (UV-Vis) transmission spectra of bacteria combined the forward scattering and absorption properties of microbes, contains substantial information on size, shape, and the other chemical, physiological character of bacterial cells, has the bacterial species specificity, which can be applied to rapid species identification of bacterial microbes. Four different kinds of bacteria including Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Klebsiella pneumonia which were commonly existed in water were researched in this paper. Their multi-wavelength UV-Vis transmission spectra were measured and analyzed.

Over-expressed copper/zinc superoxide dismutase localizes to mitochondria in neurons inhibiting the angiotensin II-mediated increase in mitochondrial superoxide.

Angiotensin II (AngII) is the main effector peptide of the renin-angiotensin system (RAS), and contributes to the pathogenesis of cardiovascular disease by exerting its effects on an array of different cell types, including central neurons. AngII intra-neuronal signaling is mediated, at least in part, by reactive oxygen species, particularly superoxide (O2 (•-)). Recently, it has been discovered that mitochondria are a major subcellular source of AngII-induced O2 (•-).

[Investigation of 1q21 amplification in patients with multiple myeloma using I-FISH and cIg-FISH].

Objective: To investigate the prevlance of 1q21 amplification in patients with multiple myeloma (MM) and its correlation with the progression and prognosis of the disease.

Methods: 1q21 amplification was detected in 48 patients with MM using cytoplasmic light chain immunofluorescence with fluorescence in situ hybridization analysis (cIg-FISH) and interphase fluorescence in situ hybridization (I-FISH) analysis combined with CD138 immunomagnetic cell sorting (MACS).

Results: 1q21 amplification (≥ 3 red signals) was detected in 26/48(54.

[Investigation of chromosome 1 aberrations in patients with multiple myeloma using cIg-FISH method and its significance].

Objective: To investigate the incidence of 1q21 amplification and 1p12 deletion, and analyze the correlation between these aberrations with disease progression, prognosis and outcome in patients with multiple myeloma (MM).

Methods: Cytoplasm light chain immunofluorescence with simultaneous interphase fluorescence in situ hybridization (cIg-FISH) was used to detecte the 1q21 amplification and 1p12 deletion in 48 patients with MM.

Results: 1q21 amplification (≥ 3 red signals) was determined in 26 of 48(54.

[mir-17-92 cluster and tumor].

MicroRNA (miRNA) is a class of RNA which has been discovered in recent years and relates with genesis and development of tumors. MiRNA affects the genesis and development of tumors and carries out the function similar to oncogene and antioncogene through regulation of signaling pathway in which target genes involved, thereby the miRNA disregulation plays an important role in oncogenesis. More studies revealed that the miR-17-19 cluster closely correlates with tumorigenesis and has bifunctional effects of oncogene and antioncogene.

Angiotensin-(1-7) increases neuronal potassium current via a nitric oxide-dependent mechanism.

Actions of angiotensin-(1-7) [Ang-(1-7)], a heptapeptide of the renin-angiotensin system, in the periphery are mediated, at least in part, by activation of nitric oxide (NO) synthase (NOS) and generation NO(·). Studies of the central nervous system have shown that NO(·) acts as a sympathoinhibitory molecule and thus may play a protective role in neurocardiovascular diseases associated with sympathoexcitation, such as hypertension and heart failure. However, the contribution of NO in the intraneuronal signaling pathway of Ang-(1-7) and the subsequent modulation of neuronal activity remains unclear.

[Investigation of the molecular changes in patients with multiple myeloma by fluorescence in situ hybridization].

Objective: To investigate the incidence and prognosis of 1q21 amplification, 13q14 deletion, TP53 gene deletion and IgH translocation in patients with multiple myeloma (MM).

Methods: Interphase fluorescence in situ hybridization (I-FISH) with four different specific probes for the regions containing 1q21, 13q14.3 (D13S319), 14q32 and TP53 gene were performed in 43 MM patients.

[Progressive transformation of lymph node germinal centers: a case report and literature review.].

Objective: To improve the understanding of progressive transformation of lymph node germinal centers (PTGC) and to explore its clinical, histopathologic and immunohistochemical features and the differential diagnosis between the related disease of germinal center hyperplasia.

Methods: The clinical manifestation, laboratory bindings, treatment and outcome of a patient with PTGC were presented.

Results: The main manifestation of the patient was painless peripheral lymphadenopathy.

The attenuation of central angiotensin II-dependent pressor response and intra-neuronal signaling by intracarotid injection of nanoformulated copper/zinc superoxide dismutase.

Adenoviral-mediated overexpression of the intracellular superoxide (O(2)(*-)) scavenging enzyme copper/zinc superoxide dismutase (CuZnSOD) in the brain attenuates central angiotensin II (AngII)-induced cardiovascular responses. However, the therapeutic potential for adenoviral vectors is weakened by toxicity and the inability of adenoviral vectors to target the brain following peripheral administration. Therefore, we developed a non-viral delivery system in which CuZnSOD protein is electrostatically bound to a synthetic poly(ethyleneimine)-poly(ethyleneglycol) (PEI-PEG) polymer to form a polyion complex (CuZnSOD nanozyme).

[microRNA-21 and microRNA-30b expression in multiple myeloma.].

Objective: To investigate the expression of miR-21 and miR-30b in multiple myeloma (MM).

Methods: Peripheral blood mononuclear cells from patients with MM were cultured at 2.5 x 10(6) cells/ml in alpha-MEM supplemented with 10% of fetal bovine serum, antibiotics, RANKL (50 ng/ml), and macrophage colony-stimulating factor (25 ng/ml) for 10 to 14 days to obtain osteoclasts with bone-resorbing activity.

Mitochondria-produced superoxide mediates angiotensin II-induced inhibition of neuronal potassium current.

Reactive oxygen species (ROS), particularly superoxide (O(2)(.-)), have been identified as key signaling intermediates in ANG II-induced neuronal activation and sympathoexcitation associated with cardiovascular diseases, such as hypertension and heart failure. Studies of the central nervous system have identified NADPH oxidase as a primary source of O(2)(.

Magnesium Lithospermate B Protects Cardiomyocytes from Ischemic Injury Via Inhibition of TAB1-p38 Apoptosis Signaling.

Danshen has been used in traditional Chinese medicine for hundreds of years to treat cardiovascular diseases. However, its precise cardioprotective components and the underlying mechanism are still unclear. In the present study, we demonstrated that in a rat model of acute myocardial infarction, the treatment with magnesium lithospermate B (MLB), the representative component of phenolic acids in Danshen, significantly reduced the infarct size and the blood lactate dehydrogenase level.

[Correlation between chromosome 13q14 deletion and 1q abnormality in multiple myeloma].

Objective: To investigate the correlation between chromosome 13q14 deletion [del(13q14)] and chromosome 1q abnormality in multiple myeloma (MM).

Methods: The bone marrow plasma cells of 48 previously untreated MM patients were purified by CD138 and magnetic cell sorting system, and interphase fluorescence in situ hybridization (I-FISH) was applied to detect the del(13q14) with D13S319 probe and the abnormalities of chromosome 1q with CEP1 SpectrumOrange probe in sorted MM cells.

Results: Among the 48 MM patients, del(13q14) was observed in 22(45.

Arterial baroreflex function does not influence telomere length in kidney of rats.

Aim: To investigate the relationship between arterial baroreflex (ABR) function and telomere length in kidney of rats.

Methods: Stroke-prone spontaneously hypertensive rats (SHR-SP) and sinoaortic denervated rats (SAD) were used as models with depressed arterial baroreflex. In the first experiments, SHR-SP rats were examined at the age of 24 weeks for both sexes and 40 weeks for female rats.

[Primary study of arsenic trioxide inhibits abdomino-metastatic tumor formation of human ovarian carcinoma in nude mice and its mechanisms].

Background And Objective: Arsenic trioxide (As2O3) has been successfully used to treat the patients with acute promyelocyutic leukemia in clinic, and for the experimental studies of liver cancer, colorectal cancer, gastric cancer, etc. The objective of this study was to explore the inhibitory effect of As2O3 on the abdomino-metastasis of human ovarian carcinoma in nude mice and its mechanisms.

Methods: Compared with cisplatin(cDDP), the growth inhibiting rates to human ovarian cancer cell line 3AO by treatment with various concentrations of As2O3 for 48 h were determined by methyl thiazolyl tetrazolium(MTT) method.