Dissemination of the (X)-Variant Genes from Layer Farms to Manure-Receiving Soil and Corresponding Lettuce.

The occurrence of high-level tigecycline resistance (X) variant genes represents a new transferable resistance crisis to food safety and human health. Here, we investigated the abundance of (X)-variant genes [(X), (X1) to (X6)] in 33 samples collected from layer manures, manured/un-manured soils, and corresponding lettuce from three provinces in China. The results showed the occurrence of (X)/(X2), (X3), and (X4) in 24 samples.

A new oncolytic adenoviral vector carrying dual tumour suppressor genes shows potent anti-tumour effect.

Cancer Targeting Gene-Viro-Therapy (CTGVT) is a promising cancer therapeutical strategy that strengthens the anti-tumour effect of oncolytic virus by expressing inserted foreign anti-tumour genes. In this work, we constructed a novel adenoviral vector controlled by the tumour-specific survivin promoter on the basis of the ZD55 vector, which is an E1B55KD gene deleted vector we previously constructed. Compared with the original ZD55 vector, this new adenoviral vector (ZD55SP/E1A) showed much better ability of replication and reporter gene expression.

Augmenting the antitumor effect of TRAIL by SOCS3 with double-regulated replicating oncolytic adenovirus in hepatocellular carcinoma.

Aberrant JAK/STAT3 pathway has been reported to be related to hepatocellular carcinoma (HCC) in many cell lines. In this study, a double-regulated oncolytic adenovirus vector that can replicate and induce a cytopathic effect in alpha-fetoprotein (AFP)-positive HCC cell lines with p53 dysfunction was successfully constructed. Two therapeutic genes, suppressor of cytokine signaling 3 (SOCS3) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), were chosen and incorporated into this vector system, respectively.

Potent antitumor effect of interleukin-24 gene in the survivin promoter and retinoblastoma double-regulated oncolytic adenovirus.

Interleukin (IL)-24 is an excellent therapeutic gene for cancer therapy. In this work, IL-24 was inserted into Ad.sp-E1A(Delta24), an oncolytic adenovirus with a 24-bp deletion in the E1A gene, which was driven by the survivin promoter to form Ad.

[Sorption characteristics of veterinary antibiotics chlortetracycline on manure].

The sorption characteristics of high-use tetracycline veterinary antibiotics-chlortetracycline (CTC) on pig manure and chicken manure were investigated in laboratory according to the OECD guideline 106. The results showed that CTC was strongly and rapidly adsorbed to the two manures and Freundlich model could describe preferably the CTC nonlinear sorption isotherms. The adsorbed CTC was difficult to be desorbed by rain with desorption H of 1.